公开(公告)号：US20130210146A1

公开(公告)日：2013-08-15

申请号：US13817091

申请日：2011-08-24

申请人： Long-Cheng Li

发明人： Long-Cheng Li

IPC分类号： C12N5/071

CPC分类号： C12N5/0696 ,  C12N15/111 ,  C12N2310/13 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606

摘要： The present disclosure provides methods for inducing somatic cells to form induced pluripotent stem (iPS) cells. The method includes introducing a small activating RNA (saRNA) molecule into the somatic cell, where the saRNA molecule increases transcription of a transcription factor that induces the formation of induced pluripotent stem cells. The present disclosure also provides compositions and kits comprising a saRNA molecule that increases transcription of a transcription factor that induces the formation of induced pluripotent stem cells. The present disclosure provides iPS cells comprising at least one exogenous saRNA molecule, where the saRNA molecule increases transcription of a transcription factor that induces the formation of induced pluripotent stem cells.

摘要翻译： 本公开提供诱导体细胞形成诱导多能干细胞（iPS）细胞的方法。 该方法包括将小活化RNA（saRNA）分子引入体细胞，其中saRNA分子增加诱导诱导的多能干细胞形成的转录因子的转录。 本公开还提供了包含增加诱导诱导的多能干细胞形成的转录因子的转录的saRNA分子的组合物和试剂盒。 本公开提供包含至少一种外源性saRNA分子的iPS细胞，其中所述saRNA分子增加诱导诱导的多能干细胞形成的转录因子的转录。

公开(公告)号：US09045752B2

公开(公告)日：2015-06-02

申请号：US13583905

申请日：2011-03-08

申请人： Long-Cheng Li

发明人： Long-Cheng Li

IPC分类号： A61K31/70 ,  C07H21/04 ,  C07H21/02 ,  C12N15/113 ,  A61K31/7105 ,  C12N15/67 ,  C12Q1/68

CPC分类号： C12N15/113 ,  A61K31/7105 ,  C12N15/67 ,  C12N2310/11 ,  C12N2310/113

摘要： The present disclosure provides compositions, pharmaceutical preparations, kits and methods for increasing expression of a NKX3-1 gene product in a cell by contacting the cell with a small activating RNA (saRNA) molecule comprising a ribonucleic strand that is complementary to a promoter region sequence of the NKX3-1 gene. The present disclosure also provides compositions, pharmaceutical preparations, kits and methods for increasing expression of a KLF4 gene product in a cell by contacting the cell with a small activating RNA (saRNA) molecule comprising a ribonucleic strand that is complementary to a promoter region sequence of the KLF4 gene.

摘要翻译： 本公开提供了通过使细胞与包含与启动子区序列互补的核糖核苷酸的小激活RNA（saRNA）分子来增加NKX3-1基因产物在细胞中的表达的组合物，药物制剂，试剂盒和方法 的NKX3-1基因。 本公开还提供了通过使细胞与包含核苷酸链的小激活RNA（saRNA）分子接触来增加细胞中KLF4基因产物的表达的组合物，药物制剂，试剂盒和方法，所述核糖核酸链与SEQ ID NO： KLF4基因。

公开(公告)号：US20130096184A1

公开(公告)日：2013-04-18

申请号：US13583905

申请日：2011-03-08

申请人： Long-Cheng Li

发明人： Long-Cheng Li

IPC分类号： C12N15/113

CPC分类号： C12N15/113 ,  A61K31/7105 ,  C12N15/67 ,  C12N2310/11 ,  C12N2310/113

摘要： The present disclosure provides compositions, pharmaceutical preparations, kits and methods for increasing expression of a NKX3-1 gene product in a cell by contacting the cell with a small activating RNA (saRNA) molecule comprising a ribonucleic strand that is complementary to a promoter region sequence of the NKX3-1 gene. The present disclosure also provides compositions, pharmaceutical preparations, kits and methods for increasing expression of a KLF4 gene product in a cell by contacting the cell with a small activating RNA (saRNA) molecule comprising a ribonucleic strand that is complementary to a promoter region sequence of the KLF4 gene.

摘要翻译： 本公开提供了通过使细胞与包含与启动子区序列互补的核糖核苷酸的小激活RNA（saRNA）分子来增加NKX3-1基因产物在细胞中的表达的组合物，药物制剂，试剂盒和方法 的NKX3-1基因。 本公开还提供了通过使细胞与包含核苷酸链的小激活RNA（saRNA）分子接触来增加细胞中KLF4基因产物的表达的组合物，药物制剂，试剂盒和方法，所述核糖核酸链与SEQ ID NO： KLF4基因。

公开(公告)号：US09045751B2

公开(公告)日：2015-06-02

申请号：US13096817

申请日：2011-04-28

申请人： Robert F. Place ,  Long-Cheng Li

发明人： Robert F. Place ,  Long-Cheng Li

IPC分类号： C07H21/02 ,  C07H21/04 ,  A61K48/00 ,  C12N15/113

CPC分类号： C12N15/113 ,  C12N15/67 ,  C12N2310/11 ,  C12N2310/533 ,  C12N2310/321 ,  C12N2310/3521

摘要： Methods, compositions and kits are provided for increasing the expression of a gene product in a cell by contacting the cell with a modified small activating RNA (saRNA) molecule, which provides for an increase in gene expression that is improved over the increase in expression provided by traditional saRNAs. These methods and compositions find use in any application in which an increase in gene expression in a cell is desired.

摘要翻译： 提供方法，组合物和试剂盒用于通过使细胞与修饰的小活化RNA（saRNA）分子接触来增加细胞中基因产物的表达，其提供增加的基因表达，所述基因表达相对于所提供的表达增加而改善 通过传统的saRNA。 这些方法和组合物可用于需要细胞中基因表达增加的任何应用中。

公开(公告)号：US20150126578A1

公开(公告)日：2015-05-07

申请号：US13096817

申请日：2011-04-28

申请人： Robert F. Place ,  Long-Cheng Li

发明人： Robert F. Place ,  Long-Cheng Li

IPC分类号： C12N15/113

CPC分类号： C12N15/113 ,  C12N15/67 ,  C12N2310/11 ,  C12N2310/533 ,  C12N2310/321 ,  C12N2310/3521

摘要： Methods, compositions and kits are provided for increasing the expression of a gene product in a cell by contacting the cell with a modified small activating RNA (saRNA) molecule, which provides for an increase in gene expression that is improved over the increase in expression provided by traditional saRNAs. These methods and compositions find use in any application in which an increase in gene expression in a cell is desired.

摘要翻译： 提供方法，组合物和试剂盒用于通过使细胞与修饰的小活化RNA（saRNA）分子接触来增加细胞中基因产物的表达，其提供增加的基因表达，所述基因表达相对于所提供的表达增加而改善 通过传统的saRNA。 这些方法和组合物可用于需要细胞中基因表达增加的任何应用中。

公开(公告)号：US20110054012A1

公开(公告)日：2011-03-03

申请号：US12810749

申请日：2008-12-23

申请人： Robert F. Place ,  Long-Cheng Li ,  Rajvir Dahiya

发明人： Robert F. Place ,  Long-Cheng Li ,  Rajvir Dahiya

IPC分类号： A61K31/7105 ,  C12N5/071 ,  C07H21/02 ,  A61P35/00 ,  C12N15/63 ,  A61K31/713

CPC分类号： C12N15/85 ,  A61K48/0066 ,  C12N15/113 ,  C12N15/63 ,  C12N2310/141 ,  C12N2330/10 ,  C12N2830/00 ,  C12N2830/85

摘要： The present invention provides compositions, pharmaceutical preparations, kits and methods for increasing expression of a gene product in a cell by contacting the cell with a microRNA (miRNA) molecule comprising a ribonucleic strand that is complementary to a non-coding nucleic acid sequence of the gene.

摘要翻译： 本发明提供组合物，药物制剂，试剂盒和方法，用于通过使细胞与包含核糖核酸链的微小RNA（miRNA）分子增加基因产物在细胞中的表达，所述微RNA（miRNA）分子互补于非编码核酸序列 基因。