Cyclomaltodextrin glucanotransferase variants
    1.
    发明授权
    Cyclomaltodextrin glucanotransferase variants 失效
    环糊精葡聚糖转化酶变体

    公开(公告)号:US6004790A

    公开(公告)日:1999-12-21

    申请号:US947965

    申请日:1997-10-09

    摘要: The present invention relates to variants of cyclomaltodextrin glucanotransferase. More specifically the invention relates to a method of modifying the substrate binding and/or product selectivity of a precursor CGTase enzyme, and CGTase variants derived from a precursor CGTase enzyme by substitution, insertion and/or deletion of one or more amino acid residue(s), which amino acid residue(s) holds a position close to the substrate. Moreover, the invention relates to DNA constructs encoding the CGTase variants, expression vectors, host cells and methods of producing the CGTase variants of the invention.

    摘要翻译: 本发明涉及环糊精葡聚糖转移酶的变体。 更具体地,本发明涉及一种通过取代,插入和/或缺失一个或多个氨基酸残基来修饰前体CGT酶的底物结合和/或产物选择性的方法和衍生自前体CGT酶的CGTase变体(s ),其中氨基酸残基保持靠近底物的位置。 此外,本发明涉及编码CGTase变体,表达载体,宿主细胞的DNA构建体和产生本发明的CGTase变体的方法。