公开(公告)号：US20120071348A1

公开(公告)日：2012-03-22

申请号：US13073871

申请日：2011-03-28

申请人： MARC C. ALBERTSEN ,  SUSAN J. MARTINO-CATT ,  XIAOMU NIU ,  IGOR CUNHA OLIVEIRA ,  DWIGHT TOMES ,  HAIYIN WANG ,  DEPING XU

发明人： MARC C. ALBERTSEN ,  SUSAN J. MARTINO-CATT ,  XIAOMU NIU ,  IGOR CUNHA OLIVEIRA ,  DWIGHT TOMES ,  HAIYIN WANG ,  DEPING XU

IPC分类号： C40B30/06

CPC分类号： C12N15/1082

摘要： The present invention provides for rapid and large scale evaluation of expression of, or function of, nucleotide sequences in plants. The invention comprises three specific components which provide for fast and large scale evaluation of nucleotide sequences. The first component includes delivery in either a single event a library of different engineered vectors or a single engineered vector for a single target nucleotide sequence comprising sequences the function of which is desired to be known in plant cells. Surprisingly, applicants have discovered that, the introduction of multiple vectors to plant cells predominantly results in individual transgenic plants which contain only a single transformation event. The second feature of the invention involves a highly transformable, fast cycling and/or miniature size plant and the final step involves mass scale analysis of T0 plants for various phenotypes and plasmid rescue to identify the nucleotide sequence present in a particular phenotype.

公开(公告)号：US20090064373A1

公开(公告)日：2009-03-05

申请号：US12200979

申请日：2008-08-29

申请人： GRAZIANA TARAMINO ,  SCOTT V. TINGEY ,  STEPHEN M. ALLEN ,  DWIGHT TOMES ,  STANLEY D. LUCK ,  HAJIME SAKAI ,  XIAOMU NIU

发明人： GRAZIANA TARAMINO ,  SCOTT V. TINGEY ,  STEPHEN M. ALLEN ,  DWIGHT TOMES ,  STANLEY D. LUCK ,  HAJIME SAKAI ,  XIAOMU NIU

IPC分类号： C12N15/82 ,  A01H5/06 ,  C12N5/14 ,  C12N15/29 ,  A01H5/00

CPC分类号： C12N15/8273 ,  C12N9/1229 ,  C12N15/8262

摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering root structure of plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a polypeptide useful for altering plant root architecture.

摘要翻译： 分离的多核苷酸和多肽以及特别可用于改变植物的根结构，包含这些重组DNA构建体的组合物（例如植物或种子）以及利用这些重组DNA构建体的方法的重组DNA构建体。 重组DNA构建体包含可操作地连接到在植物中起作用的启动子的多核苷酸，其中所述多核苷酸编码可用于改变植物根结构的多肽。

公开(公告)号：US20090158467A1

公开(公告)日：2009-06-18

申请号：US12276961

申请日：2008-11-24

申请人： DWIGHT TOMES ,  SILVIO SALVI ,  MICHELE MORGANTE ,  GIORGIO SPONZA ,  EDWARD BRUGGEMANN ,  XIAOMU NIU ,  BAILIN LI ,  ROBERTO TUBEROSA

发明人： DWIGHT TOMES ,  SILVIO SALVI ,  MICHELE MORGANTE ,  GIORGIO SPONZA ,  EDWARD BRUGGEMANN ,  XIAOMU NIU ,  BAILIN LI ,  ROBERTO TUBEROSA

IPC分类号： C12N15/82 ,  A01H5/00 ,  C12N15/11 ,  C12N15/00 ,  C12N5/04 ,  C07K14/00 ,  C12Q1/68

CPC分类号： C12N15/827 ,  C07K14/415

摘要： The present invention provides compositions and methods for modulating flowering time in plants. Maize RAP2.7 nucleotide sequences are disclosed which upon overexpression cause later flowering and when inhibited cause earlier flowering. Also disclosed is a DNA sequence which acts as a regulator/enhancer of RAP2.7, termed VGT1. This sequence does not code for any known protein, but acts as either a RNAi element or a regulatory DNA or RNA element that either directly regulates expression of flowering genes such as Rap2.7 or specifically targets expression of other genes which control flowering genes such as Rap2.7. This element this can be used as a sequence-based marker to identify inbred and hybrids which have altered maturity. Methods for expressing these nucleotide sequences in a plant for modifying maturity and flowering in plants are provided as well as expression constructs, vectors, transformed cells and plants.

摘要翻译： 本发明提供调节植物开花时间的组合物和方法。 公开了玉米RAP2.7核苷酸序列，其在过表达导致后期开花并且当被抑制时引起早期开花。 还公开了作为RAP2.7调节/增强子的DNA序列，称为VGT1。 该序列不编码任何已知的蛋白质，而是作为直接调节开花基因如Rap2.7的表达的RNAi元件或调节DNA或RNA元件，或者特异性靶向控制开花基因的其他基因的表达，例如 Rap2.7。 这个元素可以用作基于序列的标记来鉴定已经改变成熟度的近交系和杂交种。 提供用于在植物中表达这些核苷酸序列以修饰植物中的成熟和开花的方法以及表达构建体，载体，转化的细胞和植物。