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公开(公告)号：US07544857B2
公开(公告)日：2009-06-09
申请号：US11589656
申请日：2006-10-30
Applicant: Dilbag Multani , Xiaomu Niu , Dwight T. Tomes , Haiyin Wang , Deping Xu
Inventor： Dilbag Multani , Xiaomu Niu , Dwight T. Tomes , Haiyin Wang , Deping Xu
CPC classification number: C07K14/415 , C12N15/8222 , C12N15/8223
Abstract: The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.
Abstract translation: 本发明涉及基因表达的调节，特别涉及调节植物中基因表达的方法和组合物。 提供了包含可操作地连接到Brachytic2（Br2）启动子的多核苷酸分子或其功能片段或变体的表达盒。 另外提供了使用这些表达盒以及用这些表达盒转化的植物细胞，植物组织，植物和种子的方法。 所述方法和组合物用于调节植物中感兴趣的多核苷酸分子的表达。
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2.发明申请METHODS FOR LARGE SCALE FUNCTIONAL EVALUATION OF NUCLEOTIDE SEQUENCES IN PLANTS 审中-公开植物核酸序列大规模功能评估方法公开(公告)号：US20120329674A1
公开(公告)日：2012-12-27
申请号：US13312032
申请日：2011-12-06
Applicant: Marc C. Albertsen , Susan J. Martino-Catt , Xiaomu Niu , Igor Cunha Oliveira , Dwight Tomes , Haiyin Wang , Deping Xu
Inventor： Marc C. Albertsen , Susan J. Martino-Catt , Xiaomu Niu , Igor Cunha Oliveira , Dwight Tomes , Haiyin Wang , Deping Xu
IPC: C40B30/06
CPC classification number: C12N15/1082
Abstract: The present invention provides for rapid and large scale evaluation of expression of, or function of, nucleotide sequences in plants. The invention comprises three specific components which provide for fast and large scale evaluation of nucleotide sequences. The first component includes delivery in either a single event a library of different engineered vectors or a single engineered vector for a single target nucleotide sequence comprising sequences the function of which is desired to be known in plant cells. Surprisingly, applicants have discovered that, the introduction of multiple vectors to plant cells predominantly results in individual transgenic plants which contain only a single transformation event. The second feature of the invention involves a highly transformable, fast cycling and/or miniature size plant and the final step involves mass scale analysis of T0 plants for various phenotypes and plasmid rescue to identify the nucleotide sequence present in a particular phenotype.
Abstract translation: 本发明提供对植物中核苷酸序列的表达或功能的快速和大规模评估。 本发明包括提供快速和大规模评估核苷酸序列的三个特定组分。 第一组分包括在单一事件中递送不同工程化载体的文库或单个靶核苷酸序列的单一工程载体，其包含其功能期望在植物细胞中已知的序列。 令人惊讶的是，申请人已经发现，将多个载体引入植物细胞主要导致单个转基因植物，其仅包含单个转化事件。 本发明的第二个特征涉及高度可变形，快速循环和/或微型尺寸的植物，并且最终步骤涉及用于各种表型和质粒拯救的T0植物的大规模分析，以鉴定特定表型中存在的核苷酸序列。
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公开(公告)号：US20120071348A1
公开(公告)日：2012-03-22
申请号：US13073871
申请日：2011-03-28
Applicant: MARC C. ALBERTSEN , SUSAN J. MARTINO-CATT , XIAOMU NIU , IGOR CUNHA OLIVEIRA , DWIGHT TOMES , HAIYIN WANG , DEPING XU
Inventor： MARC C. ALBERTSEN , SUSAN J. MARTINO-CATT , XIAOMU NIU , IGOR CUNHA OLIVEIRA , DWIGHT TOMES , HAIYIN WANG , DEPING XU
IPC: C40B30/06
CPC classification number: C12N15/1082
Abstract: The present invention provides for rapid and large scale evaluation of expression of, or function of, nucleotide sequences in plants. The invention comprises three specific components which provide for fast and large scale evaluation of nucleotide sequences. The first component includes delivery in either a single event a library of different engineered vectors or a single engineered vector for a single target nucleotide sequence comprising sequences the function of which is desired to be known in plant cells. Surprisingly, applicants have discovered that, the introduction of multiple vectors to plant cells predominantly results in individual transgenic plants which contain only a single transformation event. The second feature of the invention involves a highly transformable, fast cycling and/or miniature size plant and the final step involves mass scale analysis of T0 plants for various phenotypes and plasmid rescue to identify the nucleotide sequence present in a particular phenotype.
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公开(公告)号：US07253000B2
公开(公告)日：2007-08-07
申请号：US11114672
申请日：2005-04-26
Applicant: Sobhana Sivasankar , Timothy J. Helentjaris , Deping Xu
Inventor： Sobhana Sivasankar , Timothy J. Helentjaris , Deping Xu
CPC classification number: C07K14/415 , C12N15/8217 , C12N15/8237 , C12N15/8238 , C12N15/8273
Abstract: The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions comprise novel nucleic acid sequences encoding a transcription factor involved in modulating gene expression in response to abiotic stress such as cold or drought. Methods for expressing the nucleic acid sequence in a plant and improving cold and/or drought tolerance of plants are also provided.
Abstract translation: 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物包含编码参与调节基因表达以响应非生物胁迫如冷或干旱的转录因子的新型核酸序列。 还提供了在植物中表达核酸序列并改善植物的耐冷和/或耐旱性的方法。
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公开(公告)号：US20070044180A1
公开(公告)日：2007-02-22
申请号：US11589656
申请日：2006-10-30
Applicant: Dilbag Multani , Xiaomu Niu , Dwight Tomes , Haiyin Wang , Deping Xu
Inventor： Dilbag Multani , Xiaomu Niu , Dwight Tomes , Haiyin Wang , Deping Xu
CPC classification number: C07K14/415 , C12N15/8222 , C12N15/8223
Abstract: The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.
Abstract translation: 本发明涉及基因表达的调节，特别涉及调节植物中基因表达的方法和组合物。 提供了包含可操作地连接到Brachytic2（Br2）启动子的多核苷酸分子或其功能片段或变体的表达盒。 另外提供了使用这些表达盒以及用这些表达盒转化的植物细胞，植物组织，植物和种子的方法。 所述方法和组合物用于调节植物中感兴趣的多核苷酸分子的表达。
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Patent Agency Ranking
公开(公告)号：US07148401B2
公开(公告)日：2006-12-12
申请号：US10931077
申请日：2004-08-31
Applicant: Dilbag Multani , Xiaomu Niu , Dwight T. Tomes , Haiyin Wang , Deping Xu
Inventor： Dilbag Multani , Xiaomu Niu , Dwight T. Tomes , Haiyin Wang , Deping Xu
CPC classification number: C07K14/415 , C12N15/8222 , C12N15/8223
Abstract: The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.
Abstract translation: 本发明涉及基因表达的调节，特别涉及调节植物中基因表达的方法和组合物。 提供了包含可操作地连接到Brachytic2（Br2）启动子的多核苷酸分子或其功能片段或变体的表达盒。 另外提供了使用这些表达盒以及用这些表达盒转化的植物细胞，植物组织，植物和种子的方法。 所述方法和组合物用于调节植物中感兴趣的多核苷酸分子的表达。
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公开(公告)号：US20060026716A1
公开(公告)日：2006-02-02
申请号：US11114672
申请日：2005-04-26
Applicant: Sobhana Sivasankar , Timothy Helentjaris , Deping Xu
Inventor： Sobhana Sivasankar , Timothy Helentjaris , Deping Xu
CPC classification number: C07K14/415 , C12N15/8217 , C12N15/8237 , C12N15/8238 , C12N15/8273
Abstract: The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions comprise novel nucleic acid sequences encoding a transcription factor involved in modulating gene expression in response to abiotic stress such as cold or drought. Methods for expressing the nucleic acid sequence in a plant and improving cold and/or drought tolerance of plants are also provided.
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8.发明申请DIRECT AND CONTINUOUS ROOT ALONE OR ROOT/SHOOT PRODUCTION FROM TRANSGENIC EVENTS DERIVED FROM GREEN REGENERATIVE TISSUES AND ITS APPLICATIONS 审中-公开从绿色再生组织衍生的转基因事件及其应用中直接和连续的根源或根/生产公开(公告)号：US20110165561A1
公开(公告)日：2011-07-07
申请号：US12977715
申请日：2010-12-23
Applicant: Myeong-Je Cho , David C. Cerf , Deping Xu , Zuo-Yu Zhao
Inventor： Myeong-Je Cho , David C. Cerf , Deping Xu , Zuo-Yu Zhao
CPC classification number: C12N15/821 , A01G22/00 , C12N15/8201 , C12N15/8227 , C12N15/8286 , G01N33/5097 , G01N2333/415 , G01N2333/43552 , Y02A40/162
Abstract: The present invention provides assays and methods for efficiently testing a polynucleotide of interest for a phenotype in a root. In some embodiments, the assays and methods include regenerating green tissue that is transgenic for at least one polynucleotide of interest into one or more transgenic plantlets that have at least one transgenic root. Further provided are methods of making a root assay by contacting green tissue with a first rooting medium to produce a plantlet and a plurality of roots. Additionally provided are methods of assaying for insecticidal activity on a live root. Accordingly provided herein is a substantially contamination-free, root bioassay. Further provided are methods of identifying a promoter having activity in a root.
Abstract translation: 本发明提供了用于有效测试根中表型的目的多核苷酸的测定和方法。 在一些实施方案中，测定和方法包括将至少一种感兴趣的多核苷酸转基因的绿色组织再生成具有至少一个转基因根的一个或多个转基因植物。 还提供了通过使绿色组织与第一生根培养基接触以产生小植物和多个根来进行根分析的方法。 另外提供了测定活根的杀虫活性的方法。 因此，本文提供了基本上无污染的根生物测定。 还提供了鉴定在根中具有活性的启动子的方法。
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9.发明授权公开(公告)号：US07928287B2
公开(公告)日：2011-04-19
申请号：US10367417
申请日：2003-02-13
Applicant: Dwight T. Tomes , Deping Xu , Haiyin Wang , Xiaomu Niu , Igor C. Oliveira
Inventor： Dwight T. Tomes , Deping Xu , Haiyin Wang , Xiaomu Niu , Igor C. Oliveira
CPC classification number: C12N15/1082
Abstract: The present invention provides for rapid and large scale evaluation of expression of, or function of, nucleotide sequences in plants. The invention comprises three specific components which provide for fast and large scale evaluation of nucleotide sequences. The first component includes delivery in either a single event a library of different engineered vectors or a single engineered vector for a single target nucleotide sequence comprising sequences the function of which is desired to be known in plant cells. Surprisingly, applicants have discovered that, the introduction of multiple vectors to plant cells predominantly results in individual transgenic plants which contain only a single transformation event. The second feature of the invention involves a highly transformable, fast cycling and/or miniature size plant and the final step involves mass scale analysis of T0 plants for various phenotypes and plasmid rescue to identify the nucleotide sequence present in a particular phenotype.
Abstract translation: 本发明提供对植物中核苷酸序列的表达或功能的快速和大规模评估。 本发明包括提供快速和大规模评估核苷酸序列的三个特定组分。 第一组分包括在单一事件中递送不同工程化载体的文库或单个靶核苷酸序列的单一工程载体，其包含其功能期望在植物细胞中已知的序列。 令人惊讶的是，申请人已经发现，将多个载体引入植物细胞主要导致单个转基因植物，其仅包含单个转化事件。 本发明的第二个特征涉及高度可变形，快速循环和/或微型尺寸的植物，并且最终步骤涉及用于各种表型和质粒拯救的T0植物的大规模分析，以鉴定特定表型中存在的核苷酸序列。
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10.发明授权公开(公告)号：US08389807B2
公开(公告)日：2013-03-05
申请号：US12649673
申请日：2009-12-30
Applicant: Zuo-Yu Zhao , Xinli E. Wu , Myoeng-Je Cho , Deping Xu
Inventor： Zuo-Yu Zhao , Xinli E. Wu , Myoeng-Je Cho , Deping Xu
CPC classification number: C12N15/8209 , A01H1/04 , C12N15/8201 , C12N15/8205
Abstract: A method for increasing efficiency of germplasm screening for transformability may include providing a plurality of lines of plant target tissue to be transformed, characterizing each of the lines to provide characterization data, the characterization data comprises DNA or nucleic acid delivery technique response data and tissue culture response data, eliminating one or more of the plurality of lines based on the characterization data without performing transformation of the plurality of lines, such that a subset of the plurality of lines remains, and performing transformation experiments on the subset of the plurality of lines. The method may also include selecting a DNA or nucleic acid delivery technique protocol and a tissue culture protocol prior to the characterization.
Abstract translation: 提高种质筛选效率的方法可包括提供要转化的多条植物靶组织，表征各条线以提供特征数据，表征数据包括DNA或核酸递送技术应答数据和组织培养 响应数据，基于所述表征数据消除所述多行中的一个或多个，而不执行所述多条线的变换，使得保留所述多条线的子集，并对所述多条线的所述子集执行变换实验。 该方法还可以包括在表征之前选择DNA或核酸递送技术方案和组织培养方案。
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