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公开(公告)号：US20120270214A1
公开(公告)日：2012-10-25
申请号：US13397503
申请日：2012-02-15
CPC分类号： C12Q1/6827 , C12Q1/6809 , C12Q1/6841 , C12Q1/6886 , C12Q2600/112 , C12Q2600/156 , C12Q2600/16 , C12Q2521/107 , C12Q2521/327 , C12Q2525/107 , C12Q2525/125 , C12Q2525/161 , C12Q2525/307 , C12Q2531/125 , C12Q2537/143 , C12Q2543/10
摘要： The present invention relates to the detection of RNA in a sample of cells. More particularly, the present invention relates to the localized detection of RNA in situ. The method relies on the conversion of RNA to complementary DNA prior to the targeting of the cDNA with a padlock probe(s). The hybridization of the padlock probe(s) relies on the nucleotide sequence of the cDNA which is derived from the corresponding nucleotide sequence of the target RNA. Rolling circle amplification of the subsequently circularized padlock probe produces a rolling circle product which may be detected. Advantageously, this allows the RNA to be detected in situ.
摘要翻译： 本发明涉及细胞样品中RNA的检测。 更具体地，本发明涉及原位RNA的局部检测。 该方法依赖于用挂锁探针靶向cDNA之前RNA到互补DNA的转化。 挂锁探针的杂交依赖于衍生自靶RNA的相应核苷酸序列的cDNA的核苷酸序列。 随后环形化的挂锁探针的滚环放大产生可被检测的滚动圆形产品。 有利地，这允许原位检测RNA。
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公开(公告)号：US08551708B2
公开(公告)日：2013-10-08
申请号：US13397503
申请日：2012-02-15
CPC分类号： C12Q1/6827 , C12Q1/6809 , C12Q1/6841 , C12Q1/6886 , C12Q2600/112 , C12Q2600/156 , C12Q2600/16 , C12Q2521/107 , C12Q2521/327 , C12Q2525/107 , C12Q2525/125 , C12Q2525/161 , C12Q2525/307 , C12Q2531/125 , C12Q2537/143 , C12Q2543/10
摘要： The present invention relates to the detection of RNA in a sample of cells. More particularly, the present invention relates to the localized detection of RNA in situ. The method relies on the conversion of RNA to complementary DNA prior to the targeting of the cDNA with a padlock probe(s). The hybridization of the padlock probe(s) relies on the nucleotide sequence of the cDNA which is derived from the corresponding nucleotide sequence of the target RNA. Rolling circle amplification of the subsequently circularized padlock probe produces a rolling circle product which may be detected. Advantageously, this allows the RNA to be detected in situ.
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公开(公告)号：US08551710B2
公开(公告)日：2013-10-08
申请号：US13542378
申请日：2012-07-05
CPC分类号： C12Q1/6827 , C12Q1/6809 , C12Q1/6841 , C12Q1/6886 , C12Q2600/112 , C12Q2600/156 , C12Q2600/16 , C12Q2521/107 , C12Q2521/327 , C12Q2525/107 , C12Q2525/125 , C12Q2525/161 , C12Q2525/307 , C12Q2531/125 , C12Q2537/143 , C12Q2543/10
摘要： The present invention relates to the detection of RNA in a sample of cells. More particularly, the present invention relates to the localized detection of RNA in situ. The method relies on the conversion of RNA to complementary DNA prior to the targeting of the cDNA with a padlock probe(s). The hybridization of the padlock probe(s) relies on the nucleotide sequence of the cDNA which is derived from the corresponding nucleotide sequence of the target RNA. Rolling circle amplification of the subsequently circularized padlock probe produces a rolling circle product which may be detected. Advantageously, this allows the RNA to be detected in situ.
摘要翻译： 本发明涉及细胞样品中RNA的检测。 更具体地，本发明涉及原位RNA的局部检测。 该方法依赖于用挂锁探针靶向cDNA之前RNA到互补DNA的转化。 挂锁探针的杂交依赖于衍生自靶RNA的相应核苷酸序列的cDNA的核苷酸序列。 随后环形化的挂锁探针的滚环放大产生可被检测的滚动圆形产品。 有利地，这允许原位检测RNA。
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公开(公告)号：US20130004953A1
公开(公告)日：2013-01-03
申请号：US13542378
申请日：2012-07-05
CPC分类号： C12Q1/6827 , C12Q1/6809 , C12Q1/6841 , C12Q1/6886 , C12Q2600/112 , C12Q2600/156 , C12Q2600/16 , C12Q2521/107 , C12Q2521/327 , C12Q2525/107 , C12Q2525/125 , C12Q2525/161 , C12Q2525/307 , C12Q2531/125 , C12Q2537/143 , C12Q2543/10
摘要： The present invention relates to the detection of RNA in a sample of cells. More particularly, the present invention relates to the localized detection of RNA in situ. The method relies on the conversion of RNA to complementary DNA prior to the targeting of the cDNA with a padlock probe(s). The hybridization of the padlock probe(s) relies on the nucleotide sequence of the cDNA which is derived from the corresponding nucleotide sequence of the target RNA. Rolling circle amplification of the subsequently circularized padlock probe produces a rolling circle product which may be detected. Advantageously, this allows the RNA to be detected in situ.
摘要翻译： 本发明涉及细胞样品中RNA的检测。 更具体地，本发明涉及原位RNA的局部检测。 该方法依赖于用挂锁探针靶向cDNA之前RNA到互补DNA的转化。 挂锁探针的杂交依赖于衍生自靶RNA的相应核苷酸序列的cDNA的核苷酸序列。 随后环形化的挂锁探针的滚环放大产生可被检测的滚动圆形产品。 有利地，这允许原位检测RNA。
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