公开(公告)号：US20220186180A1

公开(公告)日：2022-06-16

申请号：US17559314

申请日：2021-12-22

申请人： MEMORIAL SLOAN-KETTERING CANCER CENTER

发明人： Lorenz Studer ,  Stuart M. Chambers ,  Yuchen Qi ,  Yvonne Marissa Mica

IPC分类号： C12N5/0793 ,  C12N5/079 ,  G01N33/50 ,  C12N5/071

摘要： The present invention relates to the field of stem cell biology, in particular the linage specific differentiation of pluripotent or multipotent stem cells, which can include, but is not limited to, human embryonic stem cells (hESC), human induced pluripotent stem cells (hiPSC), somatic stem cells, cancer stem cells, or any other cell capable of lineage specific differentiation. Specifically described are methods to direct the lineage specific differentiation of hESC and/or hiPSC to nociceptors (i.e. nociceptor cells) using novel culture conditions. The nociceptors made using the methods of the present invention are further contemplated for various uses including, but limited to, use in in vitro drug discovery assays, pain research, and as a therapeutic to reverse disease of, or damage to, the peripheral nervous system (PNS). Further, compositions and methods are provided for producing melanocytes from human pluripotent stem cells for use in disease modeling.

公开(公告)号：US20230323294A1

公开(公告)日：2023-10-12

申请号：US18325690

申请日：2023-05-30

申请人： MEMORIAL SLOAN-KETTERING CANCER CENTER

发明人： Yuchen Qi ,  Lorenz Studer

IPC分类号： C12N5/0793 ,  A61P25/28 ,  A61K35/30

CPC分类号： C12N5/0619 ,  A61P25/28 ,  A61K35/30 ,  C12N2501/42 ,  C12N2506/03 ,  C12N2501/415 ,  C12N2501/15 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2501/155

摘要： The presently disclosed subject matter provides for in vitro methods of inducing differentiation of human stem cells into cortical neurons, and cortical neurons generated by such methods. The presently disclosed subject matter also provides for uses of such cortical neurons for treating neurodegenerative CNS disorders.

公开(公告)号：US20180346875A1

公开(公告)日：2018-12-06

申请号：US16047393

申请日：2018-07-27

申请人： MEMORIAL SLOAN-KETTERING CANCER CENTER

发明人： Yuchen Qi ,  Lorenz Studer

IPC分类号： C12N5/0793 ,  A61K35/30 ,  A61P25/28

CPC分类号： C12N5/0619 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/415 ,  C12N2501/42

摘要： The presently disclosed subject matter provides for in vitro methods of inducing differentiation of human stem cells into cortical neurons, and cortical neurons generated by such methods. The presently disclosed subject matter also provides for uses of such cortical neurons for treating neurodegenerative CNS disorders.

公开(公告)号：US20180298326A1

公开(公告)日：2018-10-18

申请号：US16014765

申请日：2018-06-21

申请人： MEMORIAL SLOAN-KETTERING CANCER CENTER

发明人： Lorenz Studer ,  Stuart M. Chambers ,  Yuchen Qi ,  Yvonne Marissa Mica

IPC分类号： C12N5/0793 ,  C12N5/079 ,  G01N33/50 ,  C12N5/071

摘要： The present invention relates to the field of stem cell biology, in particular the lineage specific differentiation of pluripotent or multipotent stem cells, which can include, but is not limited to, human embryonic stem cells (hESC), human induced pluripotent stem cells (hiPSC), somatic stem cells, cancer stem cells, or any other cell capable of lineage specific differentiation. Specifically described are methods to direct the lineage specific differentiation of hESC and/or hiPSC to nociceptors (i.e. nociceptor cells) using novel culture conditions. The nociceptors made using the methods of the present invention are further contemplated for various uses including, but limited to, use in in vitro drug discovery assays, pain research, and as a therapeutic to reverse disease of, or damage to, the peripheral nervous system (PNS). Further, compositions and methods are provided for producing melanocytes from human pluripotent stem cells for use in disease modeling.