公开(公告)号：US20080045702A1

公开(公告)日：2008-02-21

申请号：US11874560

申请日：2007-10-18

申请人： MICHAEL LANAHAN ,  Shib Basu ,  Christopher Batie ,  Wen Chen ,  Joyce Craig ,  Mark Kinkema

发明人： MICHAEL LANAHAN ,  Shib Basu ,  Christopher Batie ,  Wen Chen ,  Joyce Craig ,  Mark Kinkema

IPC分类号： C07C27/26 ,  C07H1/06 ,  C07H21/04

CPC分类号： C12N9/2428 ,  C12N9/2408 ,  C12N9/2411 ,  C12N9/2414 ,  C12N9/2422 ,  C12N9/2451 ,  C12N9/2457 ,  C12N9/246 ,  C12N15/8245 ,  C12P7/06 ,  C12P19/14 ,  C12Y302/01001 ,  C12Y302/01003 ,  C12Y302/0102 ,  C12Y302/01041 ,  C12Y302/01068 ,  Y02E50/16 ,  Y02E50/17

摘要： The invention provides polynucleotides, preferably synthetic polynucleotides, which encode processing enzymes that are optimized for expression in plants. The polynucleotides encode mesophilic, thermophilic, or hyperthermophilic processing enzymes, which are activated under suitable activating conditions to act upon the desired substrate. Also provided are “self-processing” transgenic plants, and plant parts, e.g., grain, which express one or more of these enzymes and have an altered composition that facilitates plant and grain processing. Methods for making and using these plants, e.g., to produce food products having improved taste and to produce fermentable substrates for the production of ethanol and fermented beverages are also provided.

摘要翻译： 本发明提供了多核苷酸，优选合成的多核苷酸，其编码为在植物中表达而优化的加工酶。 多核苷酸编码嗜温，嗜热或超嗜热加工酶，其在合适的活化条件下活化以作用于所需的底物。 还提供了“自加工”转基因植物和植物部分，例如表达这些酶中的一种或多种并且具有改变的组合物的植物和谷粒加工的植物部分。 还提供了制备和使用这些植物的方法，例如生产具有改善味道的食品并生产用于生产乙醇和发酵饮料的可发酵底物。