公开(公告)号：US06861578B1

公开(公告)日：2005-03-01

申请号：US09445480

申请日：1998-06-18

申请人： Maarten Anthonie Jongsma ,  Borut Strukelj ,  Brigita Lenarcic ,  Kristina Gruden ,  Vito Turk ,  Hendrik J. Bosch ,  Willem Johannes Stiekema

发明人： Maarten Anthonie Jongsma ,  Borut Strukelj ,  Brigita Lenarcic ,  Kristina Gruden ,  Vito Turk ,  Hendrik J. Bosch ,  Willem Johannes Stiekema

IPC分类号： A01H5/00 ,  A01N37/18 ,  A01N63/00 ,  A01N63/02 ,  C07K14/81 ,  C12N5/10 ,  C12N15/09 ,  C12N15/15 ,  C12N15/82

CPC分类号： C12N15/8286 ,  A01N63/00 ,  A01N63/02 ,  C07K14/8139 ,  C07K14/8142 ,  C12N15/8285 ,  Y02A40/162 ,  Y02A40/164

摘要： A method of protecting a plant or part of a plant from insect or nematode infestation is disclosed herein. The method includes the steps of: inserting into the genome of cells or tissue from a plant a sequence coding for equistatin operably linked to a promoter sequence active in the plant to cause expression of the protein at levels which provide an insect or nematode controlling amount of the protein; and regenerating resistant whole plants from the cells or tissue. Also disclosed is a transgenic plant or its sexual progeny which is resistant to attack by one or more insects or nematodes having digestive cysteine proteases. Also disclosed is an expression vehicle that includes a promotor effective to promote expression of a downstream coding sequence in plant cells, the expression vehicle being effective to express in plant cells insect controlling amounts of equistatin.

摘要翻译： 本文公开了保护植物或部分植物免受昆虫或线虫侵袭的方法。 该方法包括以下步骤：将来自植物的细胞或组织的基因组插入编码与植物中活性的启动子序列可操作地连接的等位基因的序列，以引起蛋白质的表达，其水平提供昆虫或线虫控制量 蛋白质; 并从细胞或组织中再生抗性整个植物。 还公开了一种转基因植物或其性后代，其具有抗一种或多种具有消化性半胱氨酸蛋白酶的昆虫或线虫的攻击。 还公开了一种表达载体，其包含有效促进植物细胞中下游编码序列表达的启动子，所述表达载体有效在植物细胞中表达昆虫控制量的equistatin。

公开(公告)号：US20100144893A1

公开(公告)日：2010-06-10

申请号：US12272329

申请日：2008-11-17

申请人： Asaph Aharoni ,  Maarten Anthonie Jongsma ,  Henricus Adrianus Verhoeven ,  Hendrik Jan Bouwmeester

发明人： Asaph Aharoni ,  Maarten Anthonie Jongsma ,  Henricus Adrianus Verhoeven ,  Hendrik Jan Bouwmeester

IPC分类号： A01N31/02 ,  C12N15/82 ,  A01H1/06 ,  A01P3/00

CPC分类号： C12N15/8282 ,  C12N9/1085 ,  C12N15/8243 ,  C12N15/8286 ,  Y02A40/162

摘要： The invention relates to the field of genetic engineering of flavor, fragrance and biocontrol agent development. More specifically it relates to a process for production of natural flavors, fragrances or bio-control agents by the control of one or more genes implicated in that process. The invention provides an isolated or recombinant nucleic acid or functional fragment thereof encoding a proteinaceous molecule essentially capable of flavor, fragrance and/or bio-control agent synthesis when provided with a suitable substrate under appropriate reaction conditions. The invention further provides a nucleic acid or functional fragment thereof encoding a proteinaceous molecule essentially capable of synthesizing at least a monoterpene alcohol linalool when contacted with geranyl diphosphate (GPP) and/or at least a sesquiterpene alcohol nerolidol when contacted with farnesyl diphosphate (FPP) under appropriate reaction conditions.

摘要翻译： 本发明涉及风味，香精和生物防治剂开发的遗传工程领域。 更具体地说，本发明涉及通过控制与该方法相关的一种或多种基因来生产天然香料，香料或生物控制剂的方法。 本发明提供了在合适的反应条件下提供合适的底物时，编码蛋白质分子的分离或重组的核酸或功能片段，其基本上能够进行风味，香料和/或生物控制剂的合成。 本发明进一步提供编码蛋白质分子的核酸或功能片段，当与法呢基二磷酸酯（FPP）接触时，本发明的蛋白质分子在与二香草素二磷酸酯（GPP）和/或至少一种倍半萜烯醇橙花瓶酯接触时基本能够合成至少一种单萜醇里哪醇 在适当的反应条件下。

公开(公告)号：US08338663B2

公开(公告)日：2012-12-25

申请号：US12272329

申请日：2008-11-17

申请人： Asaph Aharoni ,  Maarten Anthonie Jongsma ,  Henricus Adrianus Verhoeven ,  Hendrik Jan Bouwmeester

发明人： Asaph Aharoni ,  Maarten Anthonie Jongsma ,  Henricus Adrianus Verhoeven ,  Hendrik Jan Bouwmeester

IPC分类号： A01H5/00 ,  C12N15/29 ,  C12N15/52 ,  C12N15/82

CPC分类号： C12N15/8282 ,  C12N9/1085 ,  C12N15/8243 ,  C12N15/8286 ,  Y02A40/162

摘要： The invention relates to the field of genetic engineering of flavor, fragrance and biocontrol agent development. More specifically it relates to a process for production of natural flavors, fragrances or bio-control agents by the control of one or more genes implicated in that process. The invention provides an isolated or recombinant nucleic acid or functional fragment thereof encoding a proteinaceous molecule essentially capable of flavor, fragrance and/or bio-control agent synthesis when provided with a suitable substrate under appropriate reaction conditions. The invention further provides a nucleic acid or functional fragment thereof encoding a proteinaceous molecule essentially capable of synthesizing at least a monoterpene alcohol linalool when contacted with geranyl diphosphate (GPP) and/or at least a sesquiterpene alcohol nerolidol when contacted with farnesyl diphosphate (FPP) under appropriate reaction conditions.

摘要翻译： 本发明涉及风味，香精和生物防治剂开发的遗传工程领域。 更具体地说，本发明涉及通过控制与该方法相关的一种或多种基因来生产天然香料，香料或生物控制剂的方法。 本发明提供了在合适的反应条件下提供合适的底物时，编码蛋白质分子的分离或重组的核酸或功能片段，其基本上能够进行风味，香料和/或生物控制剂的合成。 本发明进一步提供编码蛋白质分子的核酸或功能片段，当与法呢基二磷酸酯（FPP）接触时，本发明的蛋白质分子在与二香草素二磷酸酯（GPP）和/或至少一种倍半萜烯醇橙花瓶酯接触时基本能够合成至少一种单萜醇里哪醇 在适当的反应条件下。

公开(公告)号：US07453024B2

公开(公告)日：2008-11-18

申请号：US10635223

申请日：2003-08-05

申请人： Asaph Aharoni ,  Maarten Anthonie Jongsma ,  Henricus Adrianus Verhoeven ,  Hendrik Jan Bouwmeester

发明人： Asaph Aharoni ,  Maarten Anthonie Jongsma ,  Henricus Adrianus Verhoeven ,  Hendrik Jan Bouwmeester

IPC分类号： C12N15/29 ,  C12N15/52 ,  C12N15/82 ,  A01H5/00

CPC分类号： C12N15/8282 ,  C12N9/1085 ,  C12N15/8243 ,  C12N15/8286 ,  Y02A40/162

摘要： The invention relates to the field of genetic engineering of flavor, fragrance and bio-control agent development. More specifically it relates to a process for production of natural flavors, fragrances or bio-control agents by the control of one or more genes implicated in that process. The invention provides an isolated or recombinant nucleic acid or functional fragment thereof encoding a proteinaceous molecule essentially capable of flavor, fragrance and/or bio-control agent synthesis when provided with a suitable substrate under appropriate reaction conditions. The invention further provides a nucleic acid or functional fragment thereof encoding a proteinaceous molecule essentially capable of synthesizing at least a monoterpene alcohol linalool when contacted with geranyl diphosphate (GPP) and/or at least a sesquiterpene alcohol nerolidol when contacted with farnesyl diphosphate (FPP) under appropriate reaction conditions.

摘要翻译： 本发明涉及风味，香精和生物防治剂开发基因工程领域。 更具体地说，本发明涉及通过控制与该方法相关的一种或多种基因来生产天然香料，香料或生物控制剂的方法。 本发明提供了在合适的反应条件下提供合适的底物时，编码蛋白质分子的分离或重组的核酸或功能片段，其基本上能够进行风味，香料和/或生物控制剂的合成。 本发明进一步提供编码蛋白质分子的核酸或功能片段，当与法呢基二磷酸酯（FPP）接触时，本发明的蛋白质分子在与二香草素二磷酸酯（GPP）和/或至少一种倍半萜烯醇橙花瓶酯接触时基本能够合成至少一种单萜醇里哪醇 在适当的反应条件下。

公开(公告)号：US20090031455A1

公开(公告)日：2009-01-29

申请号：US11577082

申请日：2005-10-14

申请人： Asaph Aharoni ,  Maarten Anthonie Jongsma ,  Hendrik Jan Bouwmeester

发明人： Asaph Aharoni ,  Maarten Anthonie Jongsma ,  Hendrik Jan Bouwmeester

IPC分类号： A01H5/00 ,  C12N1/21 ,  C12N7/01 ,  C12N1/15 ,  C12P7/04 ,  C12N5/10 ,  C12N5/04

CPC分类号： C12N15/8286 ,  C12N9/0071 ,  C12P7/02 ,  C12P7/22 ,  C12P7/24 ,  C12P7/40 ,  C12P7/62 ,  Y02A40/162

摘要： The present invention provides a novel cytochrome P450 enzyme, as well as vectors and recombinant host cells comprising the gene encoding the enzyme. Provided are recombinant plant with enhanced disease and/or pest resistance, modified favour and/or fragrance and methods for hydroxy lifting terpene substrates.

摘要翻译： 本发明提供了新的细胞色素P450酶，以及包含编码该酶的基因的载体和重组宿主细胞。 提供具有增强的疾病和/或害虫抗性，改良的有利和/或香料的重组植物和用于羟基提升萜烯底物的方法。