公开(公告)号：US5116748A

公开(公告)日：1992-05-26

申请号：US413431

申请日：1989-09-26

申请人： Mamoru Takahashi ,  Shinobu Nagasawa ,  Shigeru Ikuta ,  Kazuo Matsuura

发明人： Mamoru Takahashi ,  Shinobu Nagasawa ,  Shigeru Ikuta ,  Kazuo Matsuura

IPC分类号： C12N1/20 ,  C12N9/06 ,  C12R1/01

CPC分类号： C12N9/0016 ,  C12R1/01

摘要： A process for the production of L-alanine dehydrogenase which comprises culturing an L-alanine dehydrogenase producing microorganism belonging to the genus Sporolactobacillus in a nutrient medium and isolating thus-produced L-alanine dehydrogenase from the cultured mass. The microorganism is for example Sporolactobacillus sp. 78-3 FERM BP-2517 and mutants and variants thereof having the ability to produce L-alanine dehydrogenase in recoverable amounts. This strain is a thermophile which cannot grow at 40.degree. C. but can grow at 45.degree. C. and 52.degree. C. The strain can assimilate glucose and produce lactic acid and acetic acid.

公开(公告)号：US5173416A

公开(公告)日：1992-12-22

申请号：US596994

申请日：1990-10-12

申请人： Mamoru Takahashi ,  Kazuo Matsuura ,  Shinobu Nagasawa

发明人： Mamoru Takahashi ,  Kazuo Matsuura ,  Shinobu Nagasawa

IPC分类号： C12N1/20 ,  C12N9/04 ,  C12R1/05

CPC分类号： C12N9/0006 ,  Y10S435/822 ,  Y10S435/829

摘要： A new class of L-carnitine dehydrogenase is disclosed, which is stable in solution and has a residual activity of greater than 70% after one week in a pH 9.0 buffer solution at 5.degree. C., a molecular weight of 51,000.+-.6,000 daltons, a pH optimum of 9.0, a optimum temperature of 50.degree. C. and an isoelectric point of pH 5.3.+-.0.6. Also disclosed is a process for producing the new enzyme from a microorganism of the Alcaligenes genus, preferably the newly-discovered species Alcaligenes sp. No. 981 FERM BP-2570.

公开(公告)号：US5156966A

公开(公告)日：1992-10-20

申请号：US726200

申请日：1991-07-05

申请人： Mamoru Takahashi ,  Kazuo Matsuura ,  Shinobu Nagasawa

发明人： Mamoru Takahashi ,  Kazuo Matsuura ,  Shinobu Nagasawa

IPC分类号： C12N1/20 ,  C12N9/04 ,  C12R1/05

CPC分类号： C12N9/0006 ,  Y10S435/822 ,  Y10S435/829

摘要： An isolated L-carnitine dehydrogenase is disclosed, which is stable in solution and has a residual activity of greater than 70% after one week in a pH 9.0 buffer solution at 5.degree. C. Also disclosed is a process for producing the new enzyme from a microorganism of the Alcaligenes genus, preferably the newly-discovered species Alcaligenes sp. No. 981 FERM BP-2570.

摘要翻译： 公开了一种分离的L-肉碱脱氢酶，其在溶液中是稳定的，并且在5℃的pH 9.0缓冲溶液中在一周后具有大于70％的残留活性。还公开了一种从 产碱杆菌属的微生物，优选新发现的产碱杆菌属 981 FERM BP-2570。