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1.发明授权Process for the production of L-alanine dehydrogenase from 78-3 FERM BP-2517 失效从78-3 FERM BP-2517生产L-苯胺脱氢酶的方法
公开(公告)号：US5116748A
公开(公告)日：1992-05-26
申请号：US413431
申请日：1989-09-26
CPC分类号： C12N9/0016 , C12R1/01
摘要： A process for the production of L-alanine dehydrogenase which comprises culturing an L-alanine dehydrogenase producing microorganism belonging to the genus Sporolactobacillus in a nutrient medium and isolating thus-produced L-alanine dehydrogenase from the cultured mass. The microorganism is for example Sporolactobacillus sp. 78-3 FERM BP-2517 and mutants and variants thereof having the ability to produce L-alanine dehydrogenase in recoverable amounts. This strain is a thermophile which cannot grow at 40.degree. C. but can grow at 45.degree. C. and 52.degree. C. The strain can assimilate glucose and produce lactic acid and acetic acid.
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公开(公告)号：US5173416A
公开(公告)日：1992-12-22
申请号：US596994
申请日：1990-10-12
CPC分类号： C12N9/0006 , Y10S435/822 , Y10S435/829
摘要： A new class of L-carnitine dehydrogenase is disclosed, which is stable in solution and has a residual activity of greater than 70% after one week in a pH 9.0 buffer solution at 5.degree. C., a molecular weight of 51,000.+-.6,000 daltons, a pH optimum of 9.0, a optimum temperature of 50.degree. C. and an isoelectric point of pH 5.3.+-.0.6. Also disclosed is a process for producing the new enzyme from a microorganism of the Alcaligenes genus, preferably the newly-discovered species Alcaligenes sp. No. 981 FERM BP-2570.
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公开(公告)号：US5156966A
公开(公告)日：1992-10-20
申请号：US726200
申请日：1991-07-05
CPC分类号： C12N9/0006 , Y10S435/822 , Y10S435/829
摘要： An isolated L-carnitine dehydrogenase is disclosed, which is stable in solution and has a residual activity of greater than 70% after one week in a pH 9.0 buffer solution at 5.degree. C. Also disclosed is a process for producing the new enzyme from a microorganism of the Alcaligenes genus, preferably the newly-discovered species Alcaligenes sp. No. 981 FERM BP-2570.
摘要翻译： 公开了一种分离的L-肉碱脱氢酶，其在溶液中是稳定的，并且在5℃的pH 9.0缓冲溶液中在一周后具有大于70％的残留活性。还公开了一种从 产碱杆菌属的微生物，优选新发现的产碱杆菌属 981 FERM BP-2570。
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4.发明授权Nucleoside oxidase and process for making same, and process and kit for using same 失效核苷氧化酶及其制备方法，及其使用方法和试剂盒
公开(公告)号：US4385112A
公开(公告)日：1983-05-24
申请号：US292154
申请日：1981-08-12
申请人： Hideo Misaki , Shigeru Ikuta , Kazuo Matsuura
发明人： Hideo Misaki , Shigeru Ikuta , Kazuo Matsuura
IPC分类号： C07H19/04 , C07H21/00 , C12N9/04 , C12P19/28 , C12P19/38 , C12Q1/26 , C12Q1/28 , C12N9/06 , C12P7/40 , C12P13/00 , C12Q1/34 , C12Q1/42 , C12Q1/68 , C12R1/40
CPC分类号： C07H21/00 , C07H19/04 , C12N9/0006 , C12P19/28 , C12P19/38 , C12Q1/26 , C12Q1/28 , C12Y101/03028 , Y10S435/81 , Y10S435/877
摘要： A microorganism strain B-0781 belonging to the genus Pseudomonas isolated from a soil sample from an onion field in Japan, produces a novel enzyme nucleoside oxidase having substrate specificity on various nucleosides and enzyme action to catalyze enzymatic reactions involving various nucleosides. The novel nucleoside oxidase is produced by culturing a nucleoside-oxidase-producing microorganism belonging to the genus Pseudomonas in a nutrient medium containing assimilable carbon and nitrogen and inorganic salt, and isolating the thus-formed nucleoside oxidase from the cultured cells. Various nucleoside-5'-carboxylic acids can be produced, by incubating the novel nucleoside oxidase with a nucleoside having a 5'-hydroxymethyl group, in an aqueous medium under aerobic conditions, and isolating the thus-formed nucleoside-5'-carboxylic acid from the incubation medium. Assay methods for nucleosides in liquid samples are provided, which comprise incubating the sample with nucleoside oxidase, thereby consuming oxygen and generating hydrogen peroxide and nucleoside-5'-carboxylic acid by acting on nucleoside, and quantitatively determining consumed oxygen or liberated hydrogen peroxide. A kit for nucleoside assay is provided, which contains the recited ingredients.
摘要翻译： 属于从日本洋葱田土壤样品中分离出的假单胞菌属的微生物菌株B-0781产生了对各种核苷具有底物特异性和酶作用的新的酶核苷氧化酶，以催化涉及各种核苷的酶反应。 通过在含有可同化碳，氮和无机盐的营养培养基中培养属于假单胞菌属的核苷 - 氧化酶产生微生物，并从培养细胞中分离由此形成的核苷氧化酶，从而产生新的核苷氧化酶。 通过在有氧条件下在含水介质中孵育新的核苷氧化酶与具有5'-羟甲基的核苷，可以制备各种核苷-5'-羧酸，并分离由此形成的核苷-5'-羧酸 从培养基中。 提供液体样品中核苷的测定方法，其包括将样品与核苷氧化酶孵育，由此消耗氧并通过作用于核苷产生过氧化氢和核苷-5'-羧酸，并定量测定消耗的氧气或释放的过氧化氢。 提供了用于核苷测定的试剂盒，其包含所述的成分。
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公开(公告)号：US4135980A
公开(公告)日：1979-01-23
申请号：US789990
申请日：1977-04-22
申请人： Shigeru Ikuta , Yoshifumi Horiuchi , Hideo Misaki , Kazuo Matsuura , Shigeyuki Imamura , Naoki Muto
发明人： Shigeru Ikuta , Yoshifumi Horiuchi , Hideo Misaki , Kazuo Matsuura , Shigeyuki Imamura , Naoki Muto
CPC分类号： C12N9/0006 , C12N9/16 , C12Q1/32 , C12Q1/44 , C12Q1/46 , C12R1/06 , C12R1/465 , Y10S435/83
摘要： A novel enzyme choline oxidase is produced by culturing a microorganism belonging to the genus Arthrobacter, and particularly the species Arthrobacter globiformis B-0577 FERM-P No. 3518, NRRL B-11097, and isolating the choline oxidase thus produced from the culture medium. Choline oxidase is useful for the determination of choline or betaine aldehyde in a sample by mixing the new product with the sample and then measuring the generated hydrogen peroxide, betaine or consumed oxygen.
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公开(公告)号：US4346173A
公开(公告)日：1982-08-24
申请号：US201123
申请日：1980-10-27
申请人： Shigeru Ikuta , Shigeyuki Imamura , Hidehiko Ishikawa , Kazuo Matsuura , Masaki Takada , Hideo Misaki
发明人： Shigeru Ikuta , Shigeyuki Imamura , Hidehiko Ishikawa , Kazuo Matsuura , Masaki Takada , Hideo Misaki
IPC分类号： C12N9/02
CPC分类号： C12Y103/03006 , C12N9/001 , C12R1/06 , C12R1/645 , C12R1/66 , C12R1/865 , Y10S435/83 , Y10S435/911 , Y10S435/913 , Y10S435/942
摘要： A process for the production of acyl-Coenzyme A oxidase, comprises culturing an acyl-Coenzyme A-oxidase-producing microorganism belonging to genus Macrophomina, genus Cladosporium, genus Aspergillus, genus Monascus, genus Saccharomyces or genus Arthrobacter in a nutrient medium, and isolating the thus-formed acyl-CoA oxidase therefrom. The preferred species of microorganism are Macrophomina phaseoli ATCC 14383, Cladosporium resinae IFO 6367, Aspergillus candidus M-4815 FERM-P No. 5226, Monascus sp. M-4800 FERM-P No. 5225, Saccharomyces cerevisiae Y 0036 FERM-P No. 5174, and Arthrobacter sp. B-720 FERM-P No. 5224, respectively.
摘要翻译： 一种制备酰基辅酶A氧化酶的方法，包括在营养培养基中培养属于巨噬细胞，枝孢霉属，曲霉属，红曲霉属，酿酒酵母属或节杆菌属的酰基辅酶A氧化酶微生物， 由此形成的酰基辅酶A氧化酶。 微生物的优选种类是Macrophomina phaseoli ATCC 14383，Cladosporium resinae IFO 6367，Aspergillus candidus M-4815 FERM-P No.5226，Monascus sp。 M-4800 FERM-P No.5225，酿酒酵母Y 0036 FERM-P No.5174和节杆菌属（Arthrobacter sp。 B-720 FERM-P No. 5224。
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公开(公告)号：US4420562A
公开(公告)日：1983-12-13
申请号：US371458
申请日：1982-04-23
申请人： Shigeru Ikuta , Kazuo Matsuura , Hideo Misaki
发明人： Shigeru Ikuta , Kazuo Matsuura , Hideo Misaki
CPC分类号： C12R1/07 , C12N9/78 , Y10S435/815 , Y10S435/832
摘要： Isolation method of creatinase is disclosed. The method comprises culturing a microorganism belonging to Bacillus, for example, B-0618 strain (deposition No. FERM-P 4049 at Fermentation Research Institute, Agency of Industrial Science and Technology, Japan) to obtain cells from the cultured product, obtaining sarcosine oxidase and creatinase-containing solution, fractionally eluting sarcosine oxidase and creatinase by anion exchange chromatography to obtain a creatinase fraction and then collecting creatinase.
摘要翻译： 公开了肌酸酐酶的分离方法。 该方法包括培养属于芽孢杆菌的微生物，例如B-0618菌株（日本工业科学技术学院发酵研究所的淀粉编号FERM-P4049），从培养物中获得细胞，得到肌氨酸氧化酶 和含肌酸酐溶液，通过阴离子交换色谱法分级洗脱肌氨酸氧化酶和肌酸酐酶，得到肌酸酐酶级分，然后收集肌酸酐酶。
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公开(公告)号：US4216292A
公开(公告)日：1980-08-05
申请号：US946402
申请日：1978-09-27
CPC分类号： C12N9/0032 , C12Q1/26 , C12R1/07 , Y10S435/832
摘要： An enzyme sarcosine oxidase is produced by culturing a microorganism belonging to the genus Bacillus, and particularly the species Bacillus sp. B-0618, FERM-P No. 4049, NRRL No. B-11380, and isolating the sarcosine oxidase thus produced from the culture medium. Sarcosine oxidase is useful for the determination of creatinine in the presence of creatinase and creatininase in a sample by mixing the enzymes with the sample and then measuring the generated hydrogen peroxide, formaldehyde or glycine.
摘要翻译： 酶肌氨酸氧化酶是通过培养属于芽孢杆菌属的微生物，特别是芽孢杆菌属种。 B-0618，FERM-P No.4049，NRRL No.B-11380，并从培养基中分离由此产生的肌氨酸氧化酶。 肌氨酸氧化酶可用于通过将酶与样品混合，然后测量产生的过氧化氢，甲醛或甘氨酸来测定样品中肌酸酐酶和肌酸酐酶的肌酐。
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公开(公告)号：US5126246A
公开(公告)日：1992-06-30
申请号：US435003
申请日：1989-11-09
CPC分类号： C12Q1/48 , C12Q1/44 , Y10S435/81 , Y10S435/832 , Y10S436/815 , Y10S530/825 , Y10S530/83
摘要： Reagent for analysis of triglycerides contained in blood serum is provided, which comprises lipases and monoglyceride lipases capable of acting on monoglycerides having substrate specificity and capable of catalyzing the following enzymatic reaction: monoglyceride+H.sub.2 O.fwdarw.glycerol+fatty acids. The glycerol or fatty acids are measured to learn an amount of the triglycerides or fatty acid by any known analytical method.
摘要翻译： 提供了用于分析血清中含有的甘油三酯的试剂，其包含能够作用于具有底物特异性并能够催化以下酶促反应的单酸甘油酯的脂肪酶和甘油单酯脂肪酶：单酸甘油酯+ H 2 O->甘油+脂肪酸。 测量甘油或脂肪酸，以通过任何已知的分析方法学习一定量的甘油三酯或脂肪酸。
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10.发明授权Novel NAD synthetase, assay method using said novel NAD synthetase and a process for production thereof 失效新型NAD合成酶，使用所述新型NAD合成酶的测定方法及其生产方法
公开(公告)号：US4921786A
公开(公告)日：1990-05-01
申请号：US94813
申请日：1987-09-10
摘要： A novel NAD synthetase is produced by culturing a broth of Bacillus stearothermophilus H-804 FERM BP-1408. This new enzyme selectively catalyzes the reaction ##STR1## without catalyzing the reaction ##STR2## The enzyme uses ammonia or ammonium ion as a substrate, but does not use either glutamine or asparagine. Also disclosed is an assay method using the enzyme, for any one of ATP, deamide-NAD, ammonia or ammonium ion in a specimen to be assayed.
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