公开(公告)号：US20050003355A1

公开(公告)日：2005-01-06

申请号：US10420247

申请日：2003-04-22

申请人： Manchun Lu ,  Jeff Hall ,  Michael Shortreed ,  Liman Wang ,  Travis Berggren ,  Priscilla Stevens ,  David Kelso ,  Victor Lyamichev ,  Bruce Neri ,  Lloyd Smith

发明人： Manchun Lu ,  Jeff Hall ,  Michael Shortreed ,  Liman Wang ,  Travis Berggren ,  Priscilla Stevens ,  David Kelso ,  Victor Lyamichev ,  Bruce Neri ,  Lloyd Smith

IPC分类号： C12Q1/68

CPC分类号： B82Y30/00 ,  B82Y15/00 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q2565/501 ,  C12Q2561/109 ,  C12Q2565/101 ,  C12Q2521/319 ,  C12Q2565/543 ,  C12Q2537/143

摘要： Disclosed are a method and a corresponding composition of matter for detecting single nucleotide polymorphisms. The composition of matter includes a metal substrate, a probe oligonucleotide immobilized on the substrate; and an upstream oligonucleotide either immobilized on the substrate or in a solution in contact with the substrate. The probe oligonucleotide and the upstream oligonucleotide participate cooperatively in an invasive cleavage reaction when the substrate is contacted with a cleavage agent and a target nucleic acid. Under proper reactions conditions, the probe oligonucleotide, the upstream oligonucleotide, the target nucleotide, and the cleavage agent cooperative result in the formation of a cleavage product at or near the location of a single nucleotide polymorphism of interest.

摘要翻译： 公开了用于检测单核苷酸多态性的方法和相应的物质组成。 物质组成包括金属底物，固定在基底上的探针寡核苷酸; 以及固定在基底上或与基底接触的溶液中的上游寡核苷酸。 当底物与切割剂和靶核酸接触时，探针寡核苷酸和上游寡核苷酸协同参与侵入性切割反应。 在适当的反应条件下，探针寡核苷酸，上游寡核苷酸，靶核苷酸和切割剂协同导致在感兴趣的单核苷酸多态性位置处或附近形成切割产物。