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1.发明申请Single nucleotide polymorphism analysis using surface invasive cleavage reactions 审中-公开使用表面侵入性切割反应的单核苷酸多态性分析公开(公告)号：US20050003355A1
公开(公告)日：2005-01-06
申请号：US10420247
申请日：2003-04-22
申请人： Manchun Lu , Jeff Hall , Michael Shortreed , Liman Wang , Travis Berggren , Priscilla Stevens , David Kelso , Victor Lyamichev , Bruce Neri , Lloyd Smith
发明人： Manchun Lu , Jeff Hall , Michael Shortreed , Liman Wang , Travis Berggren , Priscilla Stevens , David Kelso , Victor Lyamichev , Bruce Neri , Lloyd Smith
IPC分类号： C12Q1/68
CPC分类号： B82Y30/00 , B82Y15/00 , C12Q1/6827 , C12Q1/6837 , C12Q2565/501 , C12Q2561/109 , C12Q2565/101 , C12Q2521/319 , C12Q2565/543 , C12Q2537/143
摘要： Disclosed are a method and a corresponding composition of matter for detecting single nucleotide polymorphisms. The composition of matter includes a metal substrate, a probe oligonucleotide immobilized on the substrate; and an upstream oligonucleotide either immobilized on the substrate or in a solution in contact with the substrate. The probe oligonucleotide and the upstream oligonucleotide participate cooperatively in an invasive cleavage reaction when the substrate is contacted with a cleavage agent and a target nucleic acid. Under proper reactions conditions, the probe oligonucleotide, the upstream oligonucleotide, the target nucleotide, and the cleavage agent cooperative result in the formation of a cleavage product at or near the location of a single nucleotide polymorphism of interest.
摘要翻译： 公开了用于检测单核苷酸多态性的方法和相应的物质组成。 物质组成包括金属底物，固定在基底上的探针寡核苷酸; 以及固定在基底上或与基底接触的溶液中的上游寡核苷酸。 当底物与切割剂和靶核酸接触时，探针寡核苷酸和上游寡核苷酸协同参与侵入性切割反应。 在适当的反应条件下，探针寡核苷酸，上游寡核苷酸，靶核苷酸和切割剂协同导致在感兴趣的单核苷酸多态性位置处或附近形成切割产物。
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公开(公告)号：US07485443B2
公开(公告)日：2009-02-03
申请号：US10197189
申请日：2002-07-17
申请人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd Smith
发明人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd Smith
IPC分类号： C12P19/34
CPC分类号： C12Q1/683 , C12Q2565/549 , C12Q2561/109
摘要： The present invention relates to systems, compositions, and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for attaching nucleic acids to solid supports and modifying nucleic acids. For example, in some embodiments, the 5′ nuclease activity of a cleavage agent is used to cleave a cleavage structure formed on the solid support, the occurrence of the cleavage event indicating the presence of specific nucleic acid sequences.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列变异的系统，组合物和方法。 本发明涉及将核酸连接到固体支持物和修饰核酸的方法。 例如，在一些实施方案中，切割剂的5'核酸酶活性用于切割在固体支持物上形成的切割结构，裂解事件的发生表明存在特定的核酸序列。
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公开(公告)号：US07303869B2
公开(公告)日：2007-12-04
申请号：US10269222
申请日：2002-10-11
申请人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd M. Smith
发明人： Priscilla Wilkins Stevens , David Kelso , Victor Lyamichev , Jeff Hall , Bruce P. Neri , Robert W. Kwiatkowski, Jr. , Lloyd M. Smith
CPC分类号： C12Q1/683 , C12Q2561/109 , C12Q2565/101
摘要： The present invention relates to systems, compositions, and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for attaching nucleic acids to solid supports and modifying nucleic acids. For example, in some embodiments, the 5′ nuclease activity of a cleavage agent is used to cleave a cleavage structure formed on the solid support, the occurrence of the cleavage event indicating the presence of specific nucleic acid sequences.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列变异的系统，组合物和方法。 本发明涉及将核酸连接到固体支持物和修饰核酸的方法。 例如，在一些实施方案中，切割剂的5'核酸酶活性用于切割在固体支持物上形成的切割结构，裂解事件的发生表明存在特定的核酸序列。
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公开(公告)号：US07850824B2
公开(公告)日：2010-12-14
申请号：US12572561
申请日：2009-10-02
申请人： Gary Baker , David Kelso
发明人： Gary Baker , David Kelso
IPC分类号： D21G3/00
摘要： An apparatus is described for cleaning papermaking felt by applying a low concentration of a cleaning solution through the oscillating needle nozzles. The detergent is applied intermittently while paper is being manufactured. Each cleaning period lasts for at least the length of time required for the nozzles to cover the entire surface of the felt, and preferably twice that period of time. The application of cleaning solution is then discontinued for a period of time. This cycle is repeated continuously as the paper is being manufactured. The apparatus includes a first cleaning chemical reservoir, a second cleaning chemical reservoir, a high pressure pump coupled to the first and second reservoirs, and a control unit having programming for selectively injecting the chemicals.
摘要翻译： 描述了通过将振荡针喷嘴施加低浓度的清洁溶液来清洁造纸毛毯的装置。 洗涤剂在制造纸时间断施加。 每个清洁周期持续至少喷嘴覆盖毡的整个表面所需的时间长度，并且优选为该时间段的两倍。 然后将清洁溶液的应用停止一段时间。 随着纸张的制造，连续重复此循环。 该装置包括第一清洗化学品储存器，第二清洁化学品储存器，耦合到第一和第二储存器的高压泵，以及具有用于选择性地注入化学品的编程的控制单元。
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公开(公告)号：US07918968B1
公开(公告)日：2011-04-05
申请号：US12951109
申请日：2010-11-22
申请人： Gary Baker , David Kelso
发明人： Gary Baker , David Kelso
IPC分类号： D21G3/00
摘要： An apparatus is described for cleaning papermaking felt by applying a low concentration of a cleaning solution through the oscillating needle nozzles. The detergent is applied intermittently while paper is being manufactured. Each cleaning period lasts for at least the length of time required for the nozzles to cover the entire surface of the felt, and preferably twice that period of time. The application of cleaning solution is then discontinued for a period of time. This cycle is repeated continuously as the paper is being manufactured. The apparatus includes a first cleaning chemical reservoir, a second cleaning chemical reservoir, a high pressure pump coupled to the first and second reservoirs, and a control unit having programming for selectively injecting the chemicals.
摘要翻译： 描述了通过将振荡针喷嘴施加低浓度的清洁溶液来清洁造纸毛毯的装置。 洗涤剂在制造纸时间断施加。 每个清洁周期持续至少喷嘴覆盖毡的整个表面所需的时间长度，并且优选为该时间段的两倍。 然后将清洁溶液的应用停止一段时间。 随着纸张的制造，连续重复此循环。 该装置包括第一清洗化学品储存器，第二清洁化学品储存器，耦合到第一和第二储存器的高压泵，以及具有用于选择性地注入化学品的编程的控制单元。
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6.发明授权公开(公告)号：US07597782B2
公开(公告)日：2009-10-06
申请号：US11548454
申请日：2006-10-11
申请人： Gary Baker , David Kelso
发明人： Gary Baker , David Kelso
IPC分类号： D21F1/32
摘要： A method of cleaning papermaking felt by applying a low concentration of a cleaning solution through the oscillating needle nozzles. The detergent is applied intermittently while paper is being manufactured. Each cleaning period lasts for at least the length of time required for the nozzles to cover the entire surface of the felt, and preferably twice that period of time. The application of cleaning solution is then discontinued for a period of time, generally equal to the time the cleaning detergent is applied to the felt. This cycle is repeated continuously as the paper is being manufactured. The chemistry of the cleaning solution can be changed, for example, from acidic to basic, alternately, to enhance cleaning performance.
摘要翻译： 一种通过将振动针喷嘴施加低浓度的清洁溶液来清洁造纸毡的方法。 洗涤剂在制造纸时间断施加。 每个清洁周期持续至少喷嘴覆盖毡的整个表面所需的时间长度，并且优选为该时间段的两倍。 然后将清洁溶液的使用停止一段时间，通常等于清洁洗涤剂施加到毡的时间。 随着纸张的制造，连续重复此循环。 清洗溶液的化学性能可以例如从酸性到碱性改变，以提高清洁性能。
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公开(公告)号：US20080087397A1
公开(公告)日：2008-04-17
申请号：US11548454
申请日：2006-10-11
申请人： Gary Baker , David Kelso
发明人： Gary Baker , David Kelso
摘要： A method of cleaning papermaking felt by applying a low concentration of a cleaning solution through the oscillating needle nozzles. The detergent is applied intermittently while paper is being manufactured. Each cleaning period lasts for at least the length of time required for the nozzles to cover the entire surface of the felt, and preferably twice that period of time. The application of cleaning solution is then discontinued for a period of time, generally equal to the time the cleaning detergent is applied to the felt. This cycle is repeated continuously as the paper is being manufactured. The chemistry of the cleaning solution can be changed, for example, from acidic to basic, alternately, to enhance cleaning performance.
摘要翻译： 一种通过将振动针喷嘴施加低浓度的清洁溶液来清洁造纸毡的方法。 洗涤剂在制造纸时间断施加。 每个清洁周期持续至少喷嘴覆盖毡的整个表面所需的时间长度，并且优选为该时间段的两倍。 然后将清洁溶液的使用停止一段时间，通常等于清洁洗涤剂施加到毡的时间。 随着纸张的制造，连续重复此循环。 清洗溶液的化学性能可以例如从酸性到碱性改变，以提高清洁性能。
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公开(公告)号：US07172804B2
公开(公告)日：2007-02-06
申请号：US10196909
申请日：2002-07-17
申请人： David Kelso
发明人： David Kelso
CPC分类号： B01J19/0046 , B01L3/5085 , Y10T428/24802 , Y10T428/24893
摘要： The present invention relates to film-immobilized capture particles, and substrates coated with film-immobilized capture particles. In particular, the present invention provides microarrays for detecting the presence or absence of a target in a sample employing film-immobilized capture particles. In preferred embodiments, the present provides very high density arrays that allow simultaneous analysis on a number of samples.
摘要翻译： 本发明涉及膜固定的捕获颗粒和涂覆有固定膜的捕获颗粒的底物。 特别地，本发明提供用于使用膜固定的捕获颗粒检测样品中靶的存在或不存在的微阵列。 在优选的实施方案中，本发明提供了非常高密度的阵列，其允许对多个样品进行同时分析。
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公开(公告)号：US20110061828A1
公开(公告)日：2011-03-17
申请号：US12951109
申请日：2010-11-22
申请人： Gary Baker , David Kelso
发明人： Gary Baker , David Kelso
IPC分类号： D21F1/32
摘要： An apparatus is described for cleaning papermaking felt by applying a low concentration of a cleaning solution through the oscillating needle nozzles. The detergent is applied intermittently while paper is being manufactured. Each cleaning period lasts for at least the length of time required for the nozzles to cover the entire surface of the felt, and preferably twice that period of time. The application of cleaning solution is then discontinued for a period of time. This cycle is repeated continuously as the paper is being manufactured. The apparatus includes a first cleaning chemical reservoir, a second cleaning chemical reservoir, a high pressure pump coupled to the first and second reservoirs, and a control unit having programming for selectively injecting the chemicals.
摘要翻译： 描述了通过将振荡针喷嘴施加低浓度的清洁溶液来清洁造纸毛毯的装置。 洗涤剂在制造纸时间断施加。 每个清洁周期持续至少喷嘴覆盖毡的整个表面所需的时间长度，并且优选为该时间段的两倍。 然后将清洁溶液的应用停止一段时间。 随着纸张的制造，连续重复此循环。 该装置包括第一清洗化学品储存器，第二清洁化学品储存器，耦合到第一和第二储存器的高压泵，以及具有用于选择性地注入化学品的编程的控制单元。
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公开(公告)号：US20100018662A1
公开(公告)日：2010-01-28
申请号：US12572561
申请日：2009-10-02
申请人： Gary Baker , David Kelso
发明人： Gary Baker , David Kelso
IPC分类号： D21F1/32
摘要： An apparatus is described for cleaning papermaking felt by applying a low concentration of a cleaning solution through the oscillating needle nozzles. The detergent is applied intermittently while paper is being manufactured. Each cleaning period lasts for at least the length of time required for the nozzles to cover the entire surface of the felt, and preferably twice that period of time. The application of cleaning solution is then discontinued for a period of time. This cycle is repeated continuously as the paper is being manufactured. The apparatus includes a first cleaning chemical reservoir, a second cleaning chemical reservoir, a high pressure pump coupled to the first and second reservoirs, and a control unit having programming for selectively injecting the chemicals.
摘要翻译： 描述了通过将振荡针喷嘴施加低浓度的清洁溶液来清洁造纸毛毯的装置。 洗涤剂在制造纸时间断施加。 每个清洁周期持续至少喷嘴覆盖毡的整个表面所需的时间长度，并且优选为该时间段的两倍。 然后将清洁溶液的应用停止一段时间。 随着纸张的制造，连续重复此循环。 该装置包括第一清洗化学品储存器，第二清洁化学品储存器，耦合到第一和第二储存器的高压泵，以及具有用于选择性地注入化学品的编程的控制单元。
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