公开(公告)号：US06566053B1

公开(公告)日：2003-05-20

申请号：US08579445

申请日：1995-12-27

申请人： Manuel Perucho ,  Miguel Angel Peinado ,  Yurij Ionov ,  Sergei Malkhosyan ,  Michael McClelland ,  John Welsh

发明人： Manuel Perucho ,  Miguel Angel Peinado ,  Yurij Ionov ,  Sergei Malkhosyan ,  Michael McClelland ,  John Welsh

IPC分类号： C12Q168

CPC分类号： C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/156

摘要： The detection of insertions and/or deletions in reiterated nucleotide sequences in tissues provides an identification of neoplastic changes that are associated with malignancy. The mutations are preferably detected by PCR based amplification of target sequences using selected primers, followed by standard analytic procedures. The detection of these mutations is useful as a diagnostic tool for cancer development and has direct application for cancer prognosis.

摘要翻译： 在组织中重复的核苷酸序列中的插入和/或缺失的检测提供了与恶性肿瘤相关的肿瘤变化的鉴定。 优选通过使用选择的引物的靶向序列的PCR扩增，随后进行标准分析程序来检测突变。 这些突变的检测可用作癌症发展的诊断工具，并且可以直接应用于癌症预后。

公开(公告)号：US07090978B2

公开(公告)日：2006-08-15

申请号：US10394485

申请日：2003-03-20

申请人： Manuel Perucho ,  Miguel Angel Peinado ,  Yurij Ionov ,  Sergei Malkhosyan ,  Michael McClelland ,  John Welsh

发明人： Manuel Perucho ,  Miguel Angel Peinado ,  Yurij Ionov ,  Sergei Malkhosyan ,  Michael McClelland ,  John Welsh

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/156

摘要： The detection of insertions and/or deletions in reiterated nucleotide sequences in tissues provides an identification of neoplastic changes that are associated with malignancy. The mutations are preferably detected by PCR based amplification of target sequences using selected primers, followed by standard analytic procedures. The detection of these mutations is useful as a diagnostic tool for cancer development and has direct application for cancer prognosis.

公开(公告)号：US4725550A

公开(公告)日：1988-02-16

申请号：US572694

申请日：1984-01-19

申请人： Manuel Perucho ,  Hirofumi Nakano ,  Fumiichiro Yamamoto ,  Craig Neville

发明人： Manuel Perucho ,  Hirofumi Nakano ,  Fumiichiro Yamamoto ,  Craig Neville

IPC分类号： C12N15/09 ,  A61K31/70 ,  A61P43/00 ,  C07K14/00 ,  C07K14/82 ,  C12P21/00 ,  C12N1/00 ,  C07H15/12 ,  C12N15/00

CPC分类号： C07K14/82

摘要： A c-Kirsten ras oncogene has been isolated from a human lung tumor cell line. This c-Kirsten ras has a mutation in codon 61 of the second coding exon and is capable of transforming NIH/3T3 mouse fibroblast cells to tumorigenic cells.

摘要翻译： 已经从人肺肿瘤细胞系中分离出c-Kirsten_r癌基因。 该c-Kirsten _r在第二编码外显子的密码子61中具有突变，并且能够将NIH / 3T3小鼠成纤维细胞转化成致瘤细胞。