公开(公告)号：US20100016536A1

公开(公告)日：2010-01-21

申请号：US12487243

申请日：2009-06-18

申请人： Mark A. Kelm ,  Christopher J. Johnson ,  Rebecca J. Robbins

发明人： Mark A. Kelm ,  Christopher J. Johnson ,  Rebecca J. Robbins

IPC分类号： B01D15/08 ,  C07H1/06 ,  C07K1/16 ,  C07C231/24 ,  C07C41/34

CPC分类号： B01D15/32 ,  B01J20/286 ,  B01J20/288 ,  B01J2220/54

摘要： An improved process for separating and isolating individual polar protic monomer(s) and/or oligomer(s) on the basis of degree of polymerization. A liquid sample containing polar protic monomer(s) and/or oligomer(s) is introduced into a liquid chromatography (LC) column packed with a polar bonded stationary chromatographic phase. The individual polar protic monomer(s) and/or oligomer(s) are separated via a binary mobile phase elution. One or more individual fractions containing the monomer(s) and/or oligomer(s) are eluted. The polar protic monomer(s) and/or oligomer(s) may be proanthocyanidins, hydrolyzable tannins, oligosaccharides, oligonucleotides, peptides, acrylamides, polysorbates, polyketides, poloxarners, polyethylene glycols, polyoxyethylene alcohols or polyvinyl alcohols. The binary mobile phase comprises an A phase consisting essentially of a polar aprotic solvent and a B phase consisting essentially of a polar protic solvent.A process for separating and isolating xanthine(s) (e.g., caffeine and theobromine) from polar protic monomer(s) and/or oligomer(s). A liquid sample containing xanthine(s) and polar protic monomer(s) and/or oligomer(s) is introduced into an LC column packed with a polar bonded stationary chromatographic phase. The xanthines are separated via an isocratic mobile phase elution, and one or more individual fractions containing the xanthines are eluted.

摘要翻译： 基于聚合度分离和分离各极性质子单体和/或低聚物的改进方法。 将含有极性质子单体和/或低聚物的液体样品引入填充有极性键合静止色谱相的液相色谱（LC）柱中。 单独的极性质子单体和/或低聚物通过二元流动相洗脱分离。 洗脱含有单体和/或低聚物的一种或多种单独级分。 极性质子单体和/或低聚物可以是原花青素，可水解单宁，寡糖，寡核苷酸，肽，丙烯酰胺，聚山梨醇酯，聚酮化合物，聚硅氧烷，聚乙二醇，聚氧乙烯醇或聚乙烯醇。 二元流动相包含基本上由极性非质子溶剂和基本上由极性质子溶剂组成的B相组成的A相。 从极性质子单体和/或低聚物分离和分离黄嘌呤（例如咖啡因和可可碱）的方法。 将含有黄嘌呤和极性质子单体和/或低聚物的液体样品引入填充有极性键合的静止色谱相的LC柱中。 黄嘌呤通过等度流动相洗脱分离，并且洗脱含有黄嘌呤的一个或多个单独级分。

公开(公告)号：US07566401B2

公开(公告)日：2009-07-28

申请号：US11242210

申请日：2005-10-03

申请人： Mark A. Kelm ,  Christopher J. Johnson ,  Rebecca J. Robbins

发明人： Mark A. Kelm ,  Christopher J. Johnson ,  Rebecca J. Robbins

IPC分类号： B01D15/08

CPC分类号： B01D15/32 ,  B01J20/286 ,  B01J20/288 ,  B01J2220/54

摘要： An improved process for separating and isolating individual polar protic monomer(s) and/or oligomer(s) on the basis of degree of polymerization. A liquid sample containing polar protic monomer(s) and/or oligomer(s) is introduced into a liquid chromatography (LC) column packed with a polar bonded stationary chromatographic phase. The individual polar protic monomer(s) and/or oligomer(s) are separated via a binary mobile phase elution. One or more individual fractions containing the monomer(s) and/or oligomer(s) are eluted. The polar protic monomer(s) and/or oligomer(s) may be proanthocyanidins, hydrolyzable tannins, oligosaccharides, oligonucleotides, peptides, acrylamides, polysorbates, polyketides, poloxamers, polyethylene glycols, polyoxyethylene alcohols or polyvinyl alcohols. The binary mobile phase comprises an A phase consisting essentially of a polar aprotic solvent and a B phase consisting essentially of a polar protic solvent.A process for separating and isolating xanthine(s) (e.g., caffeine and theobromine) from polar protic monomer(s) and/or oligomer(s). A liquid sample containing xanthine(s) and polar protic monomer(s) and/or oligomer(s) is introduced into an LC column packed with a polar bonded stationary chromatographic phase. The xanthines are separated via an isocratic mobile phase elution, and one or more individual fractions containing the xanthines are eluted.

摘要翻译： 基于聚合度分离和分离各极性质子单体和/或低聚物的改进方法。 将含有极性质子单体和/或低聚物的液体样品引入填充有极性键合静止色谱相的液相色谱（LC）柱中。 单独的极性质子单体和/或低聚物通过二元流动相洗脱分离。 洗脱含有单体和/或低聚物的一种或多种单独级分。 极性质子单体和/或低聚物可以是原花青素，可水解单宁，寡糖，寡核苷酸，肽，丙烯酰胺，聚山梨酯，聚酮化合物，泊洛沙姆，聚乙二醇，聚氧乙烯醇或聚乙烯醇。 二元流动相包含基本上由极性非质子溶剂和基本上由极性质子溶剂组成的B相组成的A相。 从极性质子单体和/或低聚物分离和分离黄嘌呤（例如咖啡因和可可碱）的方法。 将含有黄嘌呤和极性质子单体和/或低聚物的液体样品引入填充有极性键合的静止色谱相的LC柱中。 黄嘌呤通过等度流动相洗脱分离，并且洗脱含有黄嘌呤的一个或多个单独级分。

公开(公告)号：US07485735B2

公开(公告)日：2009-02-03

申请号：US11242473

申请日：2005-10-03

申请人： Rebecca J. Robbins ,  John P. Munafo ,  Mark A. Kelm

发明人： Rebecca J. Robbins ,  John P. Munafo ,  Mark A. Kelm

IPC分类号： C07D311/62

CPC分类号： C07D311/62 ,  C07H15/00

摘要： A process is described for selectively extracting cocoa procyanidins from an aqueous mixture of cocoa polyphenols by using a particular sequence of solvents to extract selected procyanidin monomers and/or oligomers. The solvents are n-butyl acetate, ethyl acetate, methyl acetate, diethyl ether, or mixtures of methyl acetate and diethyl ether. Preferably, the aqueous mixture of cocoa polyphenols is first extracted with n-butyl acetate. The mixtures of methyl acetate and diethyl ether are between 25:75 and 75:25 (v/v).

摘要翻译： 描述了通过使用特定的溶剂序列来提取选择的原花青素单体和/或低聚物来从可可多元酚的水性混合物中选择性提取可可原花青素的方法。 溶剂是乙酸正丁酯，乙酸乙酯，乙酸甲酯，乙醚或乙酸甲酯和乙醚的混合物。 优选地，可可多酚的含水混合物首先用乙酸正丁酯萃取。 乙酸甲酯和乙醚的混合物在25:75和75:25（v / v）之间。

公开(公告)号：US07786315B2

公开(公告)日：2010-08-31

申请号：US12342321

申请日：2008-12-23

申请人： Rebecca J. Robbins ,  Mark A. Kelm ,  John P. Munafo

发明人： Rebecca J. Robbins ,  Mark A. Kelm ,  John P. Munafo

IPC分类号： C07D311/62

CPC分类号： C07D311/62 ,  C07H15/00

摘要： A process is described for selectively extracting cocoa procyanidins from an aqueous mixture of cocoa polyphenols by using a particular sequence of solvents to extract selected procyanidin monomers and/or oligomers. The solvents are n-butyl acetate, ethyl acetate, methyl acetate, diethyl ether, or mixtures of methyl acetate and diethyl ether. Preferably, the aqueous mixture of cocoa polyphenols is first extracted with n-butyl acetate. The mixtures of methyl acetate and diethyl ether are between 25:75 and 75:25 (v/v).

摘要翻译： 描述了通过使用特定的溶剂序列来提取选择的原花青素单体和/或低聚物来从可可多元酚的水性混合物中选择性提取可可原花青素的方法。 溶剂是乙酸正丁酯，乙酸乙酯，乙酸甲酯，乙醚或乙酸甲酯和乙醚的混合物。 优选地，可可多酚的含水混合物首先用乙酸正丁酯萃取。 乙酸甲酯和乙醚的混合物在25:75和75:25（v / v）之间。

公开(公告)号：US20090203926A1

公开(公告)日：2009-08-13

申请号：US12342321

申请日：2008-12-23

申请人： Rebecca J. Robbins ,  John P. Munafo ,  Mark A. Kelm

发明人： Rebecca J. Robbins ,  John P. Munafo ,  Mark A. Kelm

IPC分类号： C07D311/62

CPC分类号： C07D311/62 ,  C07H15/00

摘要： A process is described for selectively extracting cocoa procyanidins from an aqueous mixture of cocoa polyphenols by using a particular sequence of solvents to extract selected procyanidin monomers and/or oligomers. The solvents are n-butyl acetate, ethyl acetate, methyl acetate, diethyl ether, or mixtures of methyl acetate and diethyl ether. Preferably, the aqueous mixture of cocoa polyphenols is first extracted with n-butyl acetate. The mixtures of methyl acetate and diethyl ether are between 25:75 and 75:25 (v/v).

摘要翻译： 描述了通过使用特定的溶剂序列来提取选择的原花青素单体和/或低聚物来从可可多元酚的水性混合物中选择性提取可可原花青素的方法。 溶剂是乙酸正丁酯，乙酸乙酯，乙酸甲酯，乙醚或乙酸甲酯和乙醚的混合物。 优选地，可可多酚的含水混合物首先用乙酸正丁酯萃取。 乙酸甲酯和乙醚的混合物在25:75和75:25（v / v）之间。

公开(公告)号：US20100055248A1

公开(公告)日：2010-03-04

申请号：US12305247

申请日：2006-06-28

申请人： Keith A. Woelfel ,  Rebecca J. Robbins

发明人： Keith A. Woelfel ,  Rebecca J. Robbins

IPC分类号： A23C9/152 ,  A23L2/38 ,  A23L2/46 ,  A23L2/52 ,  A23C3/02 ,  A23C9/154 ,  A23C9/156 ,  A23C9/158 ,  A23C21/00

CPC分类号： A23C9/156 ,  A23G1/32 ,  A23G1/56 ,  A23L33/105 ,  A23L33/11 ,  A23V2002/00 ,  A23V2250/2116 ,  A23V2250/21166

摘要： Shelf-stable products, particularly beverages, containing polyphenols and optionally sterol and/or stanol esters are disclosed. Loss of polyphenols during the heat processing of the products is prevented by reducing the pH of the product by at least 0.2 after mixing and prior to heat processing. The pH of the final beverage should be about pH 4.6 to about pH 6.8. The preferred products are low fat cocoa beverages containing at least 0.2-5 micrograms of cocoa polyphenols per gram of the beverage.

摘要翻译： 公开了含有多酚和任选的甾醇和/或甾烷醇酯的稳定稳定的产品，特别是饮料。 通过在混合之后和热处理之前将产物的pH降低至少0.2来防止在产品的热处理过程中多酚的损失。 最终饮料的pH应为约pH4.6至约pH6.8。 优选的产品是每克饮料含有至少0.2-5微克可可多元酚的低脂可可饮料。