公开(公告)号：US20120202189A1

公开(公告)日：2012-08-09

申请号：US12450846

申请日：2008-04-16

申请人： Mark Carle Connelly ,  Frank A.W. Coumans ,  Jimmy Page ,  Galla Chandra Rao ,  Joseph Jollick, JR.

发明人： Mark Carle Connelly ,  Frank A.W. Coumans ,  Jimmy Page ,  Galla Chandra Rao ,  Joseph Jollick, JR.

IPC分类号： G01N21/17

CPC分类号： G01N33/56983 ,  G01N33/5091 ,  G01N33/54326 ,  G01N2333/11

摘要： A novel cytometer system, methods and algorithms which provide a rugged, affordable and easy-to-use device in remote locations. All cells in a biological sample are fluorescently labeled, with target cells also magnetically labeled. Non-magnetically labeled cells are imaged for viability. Labeled sample is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells for observation. A LED illuminates the cells and a CCO camera captures the images of the fluorescent light emitted by the target cells. Image analysis performed with a novel algorithm provides a cell count that can be related to the target cell concentration of the original sample.

摘要翻译： 一种新颖的细胞仪系统，方法和算法，可在远程位置提供坚固耐用，价格实惠且易于使用的设备。 生物样品中的所有细胞都被荧光标记，靶细胞也被磁性标记。 对非磁性标记的细胞进行成像。 将标记的样品放置在两个楔形磁体之间，以选择性地移动磁性标记的细胞用于观察。 LED照亮细胞，CCO相机拍摄由靶细胞发出的荧光的图像。 用新颖算法进行的图像分析提供了可以与原始样品的靶细胞浓度相关的细胞计数。