公开(公告)号：US09080205B2

公开(公告)日：2015-07-14

申请号：US13436277

申请日：2012-03-30

申请人： Mark F. Kavlick ,  Bruce Budowle

发明人： Mark F. Kavlick ,  Bruce Budowle

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q1/6851 ,  C12Q2545/101 ,  C12Q2600/158 ,  C12Q2531/113 ,  C12Q2561/113

摘要： A real-time quantitative PCR assay that utilizes a duplex, synthetic DNA standard to ensure optimal quality assurance and quality control. One embodiment of the invention facilitates amplification of mtDNA by focusing on a 105-base pair target sequence that is minimally homologous to non-human mtDNA. The present invention can also be used to identify the presence of PCR inhibitors and thus indicate the need for sample repurification.

摘要翻译： 实时定量PCR检测，利用双工合成DNA标准，确保最佳质量保证和质量控制。 本发明的一个实施方案通过集中于与非人mtDNA最小同源的105个碱基对靶序列来促进mtDNA的扩增。 本发明还可以用于鉴定PCR抑制剂的存在，因此表明需要样品再纯化。

公开(公告)号：US20130260373A1

公开(公告)日：2013-10-03

申请号：US13436277

申请日：2012-03-30

申请人： Mark F. Kavlick ,  Bruce Budowle

发明人： Mark F. Kavlick ,  Bruce Budowle

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q1/6851 ,  C12Q2545/101 ,  C12Q2600/158 ,  C12Q2531/113 ,  C12Q2561/113

摘要： A real-time quantitative PCR assay that utilizes a duplex, synthetic DNA standard to ensure optimal quality assurance and quality control. One embodiment of the invention facilitates amplification of mtDNA by focusing on a 105-base pair target sequence that is minimally homologous to non-human mtDNA. The present invention can also be used to identify the presence of PCR inhibitors and thus indicate the need for sample repurification.