公开(公告)号：US4912041A

公开(公告)日：1990-03-27

申请号：US247780

申请日：1988-09-21

申请人： Mark J. Batchelor ,  Stephen C. Williams ,  Mokina J. Green

发明人： Mark J. Batchelor ,  Stephen C. Williams ,  Mokina J. Green

IPC分类号： C12Q1/00 ,  G01N27/49

CPC分类号： C12Q1/005 ,  Y10S435/81

摘要： A method of assay for the presence of .alpha.-amylase in a sample employs a derivatized .alpha.-oligosaccharide which can be hydrolyzed by an enzyme mix including .alpha.-amylase to yield an electroactive compound, and at least one other enzyme which with .alpha.-amylase gives an enzyme mix capable of hydrolyzing the derivatized .alpha.-oligosaccharide. The sample is contacted with the derivatized .alpha.-oligosaccharide and with the other enzyme to permit hydrolysis of the derivatized .alpha.-oligosaccharide and to allow formation of the electroactive compound. An electrode is poised at a potential at which the derivatized .alpha.-oligosaccharide is not electochemically active, the potential being one at which the electroactive compound is electochemically active. Any current arising from electrochemical activity of the electroactive compound is detected.

公开(公告)号：US07150975B2

公开(公告)日：2006-12-19

申请号：US10643631

申请日：2003-08-18

申请人： Janet A. Tamada ,  Michael J. Tierney ,  Stephen C. Williams

发明人： Janet A. Tamada ,  Michael J. Tierney ,  Stephen C. Williams

IPC分类号： C12Q1/54

CPC分类号： A61B5/1486 ,  A61N1/0436 ,  A61N1/044 ,  A61N1/325 ,  C08J3/075

摘要： The present invention relates to compositions for use in analyte monitoring devices. These compositions are useful to increase the flux of analyte across skin, tissue or mucosal surfaces. The compositions include hydrogels and collection reservoir systems comprising ionically conductive materials. The present invention also includes methods of making/manufacturing hydrogels or collection reservoir systems, collection assemblies comprising the hydrogels, electrode assemblies in combination with the hydrogels or collection reservoir systems, and methods of using the same.

摘要翻译： 本发明涉及用于分析物监测装置的组合物。 这些组合物可用于增加分析物通过皮肤，组织或粘膜表面的通量。 组合物包括包含离子导电材料的水凝胶和收集储存器系统。 本发明还包括制造/制造水凝胶或收集储存器系统的方法，包括水凝胶的收集组件，与水凝胶或收集储存器系统组合的电极组件及其使用方法。

公开(公告)号：US5126034A

公开(公告)日：1992-06-30

申请号：US383202

申请日：1989-07-21

申请人： Nigel F. Carter ,  Christopher J. Hammond ,  Monika J. Green ,  Paul I. Hilditch ,  Stephen C. Williams

发明人： Nigel F. Carter ,  Christopher J. Hammond ,  Monika J. Green ,  Paul I. Hilditch ,  Stephen C. Williams

IPC分类号： G01N27/28 ,  C12Q1/00 ,  C12Q1/04 ,  G01N27/30 ,  G01N27/416 ,  G01N33/543

CPC分类号： C12Q1/001 ,  C12Q1/04 ,  G01N33/5438 ,  Y10S436/806

摘要： A disposable element is provided for use in analytical equipment for electrochemical determination of biological cells. The disposable element comprises a working electrode, a reference electrode, and a filter for retention of solids in the vicinity of the electrodes. The analytical equipment with disposable elements can be used for respiratory assays, enzyme assays or immunoassays, among other uses.In a variation, the disposable element comprises a reference electrode, working electrode and fusible link.

摘要翻译： 提供一次性元件用于生物细胞的电化学测定的分析设备中。 一次性元件包括工作电极，参考电极和用于在电极附近保持固体的过滤器。 具有一次性元件的分析设备可用于呼吸测定，酶测定或免疫测定，以及其他用途。 在一个变型中，一次性元件包括参考电极，工作电极和可熔连接件。