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1.发明授权High level expression and facile purification of proteins, peptides and conjugates for immunization, purification and detection applications 失效用于免疫，纯化和检测应用的蛋白质，肽和缀合物的高水平表达和易于纯化
公开(公告)号：US6069230A
公开(公告)日：2000-05-30
申请号：US338382
申请日：1994-11-10
IPC分类号： G01N33/531 , A61K38/00 , A61K39/00 , A61P37/02 , A61P43/00 , C07K14/00 , C07K14/01 , C07K16/18 , C07K16/28 , C07K19/00 , C12N1/21 , C12N15/09 , C12N15/62 , C12P21/02 , C12Q1/25 , G01N33/543
CPC分类号： C07K14/005 , C07K16/18 , C07K16/2866 , C12N15/625 , A61K39/00 , C07K2317/11 , C07K2317/23 , C07K2319/02 , C07K2319/09 , C07K2319/10 , C07K2319/40 , C12N2795/10222
摘要： A process for pro and purifying peptides and for producing peptide/protein antigens for antibody production is described. The process utilizes fusion proteins and specifically fusion proteins in which the fusion protein carrier segment includes an amino acid sequence at least about 65 amino acids long and in which the amino acid sequence does not contain negative or positive charged side chains of amino acids.
摘要翻译： 描述了用于促进和纯化肽和产生用于抗体生产的肽/蛋白质抗原的方法。 该方法利用融合蛋白和特异性融合蛋白，其中融合蛋白载体片段包含至少约65个氨基酸长的氨基酸序列，其中氨基酸序列不含有负氨基酸或带正电荷的氨基酸侧链。
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2.发明授权High level expression and facile purification of proteins, peptides and conjugates for immunization, purification and detection applications 失效用于免疫，纯化和检测应用的蛋白质，肽和缀合物的高水平表达和易于纯化公开(公告)号：US06764689B1
公开(公告)日：2004-07-20
申请号：US09345264
申请日：1999-06-30
IPC分类号： A61K3939
CPC分类号： C07K14/005 , A61K39/00 , C07K16/18 , C07K16/2866 , C07K2317/11 , C07K2317/23 , C07K2319/02 , C07K2319/09 , C07K2319/10 , C07K2319/40 , C12N15/625 , C12N2795/10222
摘要： A process for producing and purifying peptides and for producing peptide/protein antigens for antibody production is described. The process utilizes fusion proteins and specifically fusion proteins in which the fusion protein carrier segment includes an amino acid sequence at least about 65 amino acids long and in which the amino acid sequence does not contain negative or positive charged side chains of amino acids.
摘要翻译： 描述了用于产生和纯化肽和用于产生用于抗体生产的肽/蛋白质抗原的方法。 该方法利用融合蛋白和特异性融合蛋白，其中融合蛋白载体片段包含至少约65个氨基酸长的氨基酸序列，其中氨基酸序列不含有负氨基酸或带正电荷的氨基酸侧链。
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3.发明授权High level expression and facile purification of proteins, peptides and conjugates for immunization, purification and detection applications 失效用于免疫，纯化和检测应用的蛋白质，肽和缀合物的高水平表达和易于纯化
公开(公告)号：US5989554A
公开(公告)日：1999-11-23
申请号：US174060
申请日：1998-10-16
IPC分类号： G01N33/531 , A61K38/00 , A61K39/00 , A61P37/02 , A61P43/00 , C07K14/00 , C07K14/01 , C07K16/18 , C07K16/28 , C07K19/00 , C12N1/21 , C12N15/09 , C12N15/62 , C12P21/02 , C12Q1/25 , G01N33/543 , A61K39/385
CPC分类号： C07K14/005 , C07K16/18 , C07K16/2866 , C12N15/625 , A61K39/00 , C07K2317/11 , C07K2317/23 , C07K2319/02 , C07K2319/09 , C07K2319/10 , C07K2319/40 , C12N2795/10222
摘要： A process for producing and purifying peptides and for producing peptide/protein antigens for antibody production is described. The process utilizes fusion proteins and specifically fusion proteins in which the fusion protein carrier segment includes an amino acid sequence at least about 65 amino acids long and in which the amino acid sequence does not contain negative or positive charged side chains of amino acids.
摘要翻译： 描述了用于产生和纯化肽和用于产生用于抗体生产的肽/蛋白质抗原的方法。 该方法利用融合蛋白和特异性融合蛋白，其中融合蛋白载体片段包含至少约65个氨基酸长的氨基酸序列，其中氨基酸序列不含有负氨基酸或带正电荷的氨基酸侧链。
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4.发明授权Site-specific mutagenesis and mutant selection utilizing antibiotic-resistant markers encoding gene products having altered substrate specificity 失效利用抗生素抗性标记编码具有改变的底物特异性的基因产物的位点特异性诱变和突变选择
公开(公告)号：US5780270A
公开(公告)日：1998-07-14
申请号：US682073
申请日：1996-07-17
申请人： Scott A. Lesley
发明人： Scott A. Lesley
CPC分类号： C12N9/86 , C12N15/102 , Y10S435/81
摘要： Methods, kits, and reagents for conducting site-specific mutagenesis of single or double-stranded nucleic acids which utilizes novel antibiotic resistance conferred by a mutated antibiotic resistance gene for efficient mutant selection are described.
摘要翻译： 描述了用于进行单链或双链核酸的位点特异性诱变的方法，试剂盒和试剂，其利用由突变的抗生素抗性基因赋予的新抗生素抗性用于有效的突变体选择。
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公开(公告)号：US06194562B1
公开(公告)日：2001-02-27
申请号：US09064449
申请日：1998-04-22
申请人： Craig E. Smith , Donald A. Creswell , Rex M. Bitner , Douglas H. White , Braeden L. Butler , Scott A. Lesley
发明人： Craig E. Smith , Donald A. Creswell , Rex M. Bitner , Douglas H. White , Braeden L. Butler , Scott A. Lesley
IPC分类号： C07H2100
CPC分类号： C12N15/1013 , C12N15/1006
摘要： The present invention presents a novel method for removing endotoxins from nucleic acids, such as DNA, RNA, or hybrids thereof, contaminated therewith. Nucleic acid solutions which can be treated using the method of this invention include, but are not limited to, lysates of gram-negative bacteria and nucleic acid solutions contaminated with endotoxins from external sources. The present method removes endotoxins from such solutions using silica-based materials, such as silica gel particles, magnetic silica particles, or diatomaceous earth. In a preferred aspect of the method of this invention, magnetic silica particles are used to isolate plasmid DNA from a lysate of gram-negative bacteria transformed with the plasmid DNA. Application of the disclosed method produces nucleic acids which are sufficiently free of endotoxin contamination to be useful for a variety of different practical applications.
摘要翻译： 本发明提供了一种用于从被污染的核酸如DNA，RNA或其杂交体中除去内毒素的新方法。 可以使用本发明的方法治疗的核酸溶液包括但不限于革兰氏阴性细菌的裂解物和被来自外部来源的内毒素污染的核酸溶液。 本发明的方法使用二氧化硅基材料，例如硅胶颗粒，磁性二氧化硅颗粒或硅藻土从这种溶液中除去内毒素。 在本发明方法的优选方面，使用磁性二氧化硅颗粒从质粒DNA转化的革兰氏阴性细菌的裂解物中分离出质粒DNA。 所公开方法的应用产生足够不含内毒素污染物的核酸可用于各种不同的实际应用。
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