公开(公告)号：US20130330749A1

公开(公告)日：2013-12-12

申请号：US13968292

申请日：2013-08-15

申请人： Masahiro SUGIMOTO ,  Tomoyoshi SOGA

发明人： Tomoyoshi SOGA ,  Masahiro SUGIMOTO ,  Makoto SUEMATSU ,  Masashi HONMA ,  Takehito YAMAMOTO ,  Hiroshi SUZUKI

IPC分类号： G01N33/487 ,  G01N33/497

CPC分类号： G01N33/487 ,  C07K5/0215 ,  G01N33/497 ,  G01N33/576 ,  G01N33/6812 ,  G01N33/6893 ,  G01N2800/08 ,  G01N2800/60

摘要： A normal person (i.e. a control) and liver diseases such as drug induced liver injury, an asymptomatic hepatitis B carrier, an asymptomatic hepatitis C carrier, chronic hepatitis B, chronic hepatitis C, liver cancer, a nonalcoholic fatty liver disease (NAFLD), nonalcoholic steatohepatitis (NASH), and simple steatosis (SS) are identified by measuring the concentrations of γ-Glu-X (X represents an amino acid or an amine) peptides or the levels of AST or ALT in blood and carrying out, for example, a multiple logistic regression based on the measured value.

摘要翻译： 正常人（即对照）和肝脏疾病如药物诱发的肝损伤，无症状乙型肝炎携带者，无症状丙型肝炎携带者，慢性乙型肝炎，慢性丙型肝炎，肝癌，非酒精性脂肪性肝病（NAFLD） 通过测量血液中的γ-Glu-X（X代表氨基酸或胺）肽的浓度或AST或ALT的水平，进行例如，通过测定非酒精性脂肪性肝炎（NASH）和简单脂肪变性（SS） ，基于测量值的多重逻辑回归。

公开(公告)号：US20110259743A1

公开(公告)日：2011-10-27

申请号：US13092420

申请日：2011-04-22

申请人： Tomoyoshi SOGA ,  Makoto Suematsu

发明人： Tomoyoshi SOGA ,  Makoto Suematsu

IPC分类号： G01N27/447 ,  H01J49/26

CPC分类号： G01N33/6893 ,  G01N2430/00 ,  G01N2500/00 ,  G01N2800/00

摘要： Provided is a biomarker that enables easy and rapid detection of oxidative stress on a living organism and enables prevention of tissue damage or cell necrosis by drug administration, and which is a powerful marker for the study of toxicity and pharmacokinetics of various agents. Oxidative stress is determined by measuring blood concentration of ophthalmic acid, which is a substance that varies in blood depending on the variation of reduced glutathione (GSH) concentration in a biological sample with the use of an analyzer such as a capillary electrophoresis-mass spectrometer. Further, an anti-oxidative stress agent is screened by administering an anti-oxidative stress candidate agent to a non-human animal under oxidative stress conditions, measuring blood concentration of ophthalmic acid, and evaluating the degree of decrease in the ophthalmic acid concentration.

摘要翻译： 提供了能够容易且快速地检测活体上的氧化应激的生物标志物，并且能够通过药物给药预防组织损伤或细胞坏死，并且其是研究各种药剂的毒性和药代动力学的有力标记。 通过使用诸如毛细管电泳 - 质谱仪的分析仪，通过测量根据生物样品中还原型谷胱甘肽（GSH）浓度的变化的血液变化的眼睛液的血液浓度来测定氧化应激。 此外，通过在氧化应激条件下向非人动物施用抗氧化应激候选试剂，测定眼酸的血药浓度，评价眼酸浓度的降低程度来筛选抗氧化应激剂。