公开(公告)号：US20110142810A1

公开(公告)日：2011-06-16

申请号：US13059714

申请日：2009-02-20

申请人： Masahiro Saito ,  Shinya Murakami ,  Toshiyuki Yoneda ,  Tomoko Hashikawa

发明人： Masahiro Saito ,  Shinya Murakami ,  Toshiyuki Yoneda ,  Tomoko Hashikawa

IPC分类号： A61K35/32 ,  C12N5/077 ,  A61P19/00

CPC分类号： A61L27/3821 ,  A61K35/00 ,  A61K35/12 ,  A61L27/3847 ,  A61L27/3865 ,  A61L2430/02 ,  C12N5/0654 ,  C12N2501/115 ,  C12N2501/135

摘要： The present invention provides a cell preparation that can be prepared from cells collected from a patient of any age group, and that has an excellent bone tissue regenerative capacity and is effective for bone tissue regeneration. Undifferentiated osteoblasts obtained from alveolar bone, particularly those obtained by being cultured after the enzyme treatment of alveolar bone have a remarkable bone tissue regenerative capacity, and are therefore useful for a cell preparation for bone tissue regeneration.

摘要翻译： 本发明提供可以从任何年龄组的患者收集的细胞制备的细胞制剂，并且具有优异的骨组织再生能力并且对于骨组织再生有效。 从牙槽骨获得的未分化成骨细胞，特别是通过在牙槽骨的酶处理后培养获得的成骨细胞具有显着的骨组织再生能力，因此可用于骨组织再生的细胞制备。

公开(公告)号：US20100068188A1

公开(公告)日：2010-03-18

申请号：US12311275

申请日：2007-09-20

申请人： Shinya Murakami ,  Tomoko Hashikawa ,  Yoshiki Sawa ,  Akifumi Matsuyama ,  Hiroshi Komoda

发明人： Shinya Murakami ,  Tomoko Hashikawa ,  Yoshiki Sawa ,  Akifumi Matsuyama ,  Hiroshi Komoda

IPC分类号： A61K45/00 ,  C12N5/071 ,  A61P1/02

CPC分类号： A61L27/3865 ,  A61K35/12 ,  A61L27/3834 ,  A61L27/3895 ,  A61L2430/12 ,  C12N5/0654 ,  C12N2500/38 ,  C12N2500/42 ,  C12N2501/30 ,  C12N2501/39 ,  C12N2506/1384

摘要： An object of the present invention is to provide technology of using a preadipocyte to restore comprehensively bone, cartilage and periodontal ligament, and cause a periodontal hard tissue to regenerate. By culturing a preadipocyte using a culture medium containing (i) at least one member selected from the group consisting of glycerophosphate and salts thereof, and (ii) at least one member selected from the group consisting of ascorbic acid, derivatives thereof and salts thereof and not containing an adrenal cortical hormone in such an amount that promotes differentiation of a preadipocyte into a periodontal hard tissue cell, differentiation of the cell into a periodontal hard tissue cell is induced efficiently. Furthermore, a scaffold is combined with the preadipocyte cultured with the above-mentioned culture medium to be transplanted into a periodontal tissue that requires regeneration.

摘要翻译： 本发明的目的是提供使用前脂肪细胞全面恢复骨，软骨和牙周韧带并使牙周硬组织再生的技术。 通过使用培养基培养前脂肪细胞，所述培养基含有（i）选自甘油磷酸盐及其盐的至少一种，和（ii）至少一种选自抗坏血酸，其衍生物和盐及其盐的成员，以及 不含有促进前脂肪细胞分化为牙周硬组织细胞的量的肾上腺皮质激素，有效诱导细胞向牙周硬组织细胞的分化。 此外，将支架与与上述培养基一起培养的前脂肪细胞组合以移植到需要再生的牙周组织中。