公开(公告)号：US09134235B2

公开(公告)日：2015-09-15

申请号：US13817356

申请日：2011-07-27

申请人： Masanori Tsukagoshi ,  Takatoshi Kaya ,  Noriaki Yamamoto

发明人： Masanori Tsukagoshi ,  Takatoshi Kaya ,  Noriaki Yamamoto

IPC分类号： G01N21/64 ,  G01N21/05 ,  G01N33/543

CPC分类号： G01N21/6402 ,  G01N21/05 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/54393

摘要： [Object] It is an object of the invention to provide a sensor area which can suppress a decrease in assay signal and an increase in assay blank in an SPFS measurement.[Solution] An SPFS sensor chip of the invention includes a purification area and a sensor area arranged upstream and downstream, respectively, relative to each other along a flow direction in a channel for surface plasmon-field enhanced fluorescence spectroscopy [SPFS].

摘要翻译： 发明内容本发明的目的是提供一种传感器区域，其可以抑制测定信号的降低和SPFS测量中测定空白的增加。 [解决方案]本发明的SPFS传感器芯片包括分别在表面等离激元增强荧光光谱通道（SPFS）中沿着流动方向相对于彼此布置的上游和下游的净化区域和传感器区域。

公开(公告)号：US20130143332A1

公开(公告)日：2013-06-06

申请号：US13817356

申请日：2011-07-27

申请人： Masanori Tsukagoshi ,  Takatoshi Kaya ,  Noriaki Yamamoto

发明人： Masanori Tsukagoshi ,  Takatoshi Kaya ,  Noriaki Yamamoto

IPC分类号： G01N21/64

CPC分类号： G01N21/6402 ,  G01N21/05 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/54393

摘要： [Object] It is an object of the invention to provide a sensor area which can suppress a decrease in assay signal and an increase in assay blank in an SPFS measurement.[Solution] An SPFS sensor chip of the invention includes a purification area and a sensor area arranged upstream and downstream, respectively, relative to each other along a flow direction in a channel for surface plasmon-field enhanced fluorescence spectroscopy [SPFS].

摘要翻译： 发明内容本发明的目的是提供一种传感器区域，其可以抑制测定信号的降低和SPFS测量中测定空白的增加。 [解决方案]本发明的SPFS传感器芯片包括分别在表面等离激元增强荧光光谱通道（SPFS）中沿着流动方向相对于彼此布置的上游和下游的净化区域和传感器区域。

公开(公告)号：US09335324B2

公开(公告)日：2016-05-10

申请号：US13353508

申请日：2012-01-19

申请人： Noriaki Yamamoto ,  Takatoshi Kaya

发明人： Noriaki Yamamoto ,  Takatoshi Kaya

IPC分类号： G01N33/553 ,  G01N21/64 ,  G01N33/543

CPC分类号： G01N33/553 ,  G01N21/6428 ,  G01N21/648 ,  G01N33/54373 ,  G01N2610/00

摘要： An assay method with use of a sensor chip which includes a metal member, a self-assembled monolayer (SAM), and ligands on a support, and is configured to be used for a fluorescence measuring apparatus with utilization of a surface plasmon-field enhanced Fluorescence Spectrometry, including the steps of: forming a hydrophilic high molecule layer on the self-assembled monolayer in the sensor chip; immobilizing the ligands at least one of in the hydrophilic high molecule layer and on the surface of the hydrophilic high molecule layer; and bringing a moisturizer in contact with the hydrophilic high molecule layer.

摘要翻译： 一种使用包括金属部件，自组装单层（SAM）和载体上的配体的传感器芯片的测定方法，并且被配置为用于使用表面等离子体场增强的荧光测量装置 荧光光谱法，包括以下步骤：在传感器芯片上的自组装单层上形成亲水性高分子层; 将配体固定在亲水性高分子层和亲水性高分子层表面上的至少一种; 并使保湿剂与亲水性高分子层接触。

公开(公告)号：US20130078148A1

公开(公告)日：2013-03-28

申请号：US13702459

申请日：2011-06-06

申请人： Takatoshi Kaya ,  Naoki Hikage ,  Hidetaka Ninomiya

发明人： Takatoshi Kaya ,  Naoki Hikage ,  Hidetaka Ninomiya

IPC分类号： G01N21/64

CPC分类号： G01N21/64 ,  C03C17/40 ,  G01N21/6428 ,  G01N21/648 ,  G01N33/54373 ,  G01N2021/6439 ,  G01N2610/00

摘要： A surface plasmon-field enhanced fluorescence spectroscopy [SPFS] sensor chip may include a transparent support, a metal thin film formed on one surface of the transparent support, a self-assembled monolayer [SAM] formed on a surface of the metal thin film, said surface not being in contact with the transparent support, a solid phase layer formed on a surface of the SAM and having a three-dimensional structure, said surface not being in contact with the metal thin film, and a ligand immobilized in the solid phase layer. A fluctuation ratio represented by the following formula is not less than 0% but not more than 30%: {half-width (α)−half-width (β)}/half-width (β)×100.

摘要翻译： 表面等离子体场增强荧光光谱[SPFS]传感器芯片可以包括透明支撑体，形成在透明支撑体的一个表面上的金属薄膜，在金属薄膜的表面上形成的自组装单层[SAM] 所述表面不与透明支撑体接触，形成在SAM表面上并具有三维结构的固相层，所述表面不与金属薄膜接触，并且固定在固相中的配体 层。 由下式表示的波动比不小于0％但不大于30％：{半宽（α） - 半峰宽（＆bgr）} /半宽（＆bgr）×100。

公开(公告)号：US20140117255A1

公开(公告)日：2014-05-01

申请号：US14126752

申请日：2012-05-31

申请人： Naoki Hikage ,  Masataka Matsuo ,  Takatoshi Kaya

发明人： Naoki Hikage ,  Masataka Matsuo ,  Takatoshi Kaya

IPC分类号： G01N21/64

CPC分类号： G01N21/64 ,  B01L3/502715 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/168 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/582 ,  G01N2021/6417 ,  G01N2021/6439 ,  G01N2021/6482

摘要： To provide a surface plasmon-field enhanced fluorescence spectroscopic measurement method and a surface plasmon-field enhanced fluorescence spectroscopic measurement device which are capable of accurately measuring a fluorescent signal regardless of the type of a light detection means even when the concentration of an analyte is high by adjusting the dynamic range of the SPFS device. A surface plasmon-field enhanced fluorescence stereoscopic measurement method wherein an analyte labeled with a fluorescent substance is excited by surface plasmon light generated by applying excitation light to a metallic thin film, and generated fluorescence is received by a light detection means to thereby detect the analyte. The dynamic range is expanded by adjusting the amount of the fluorescence received by the light detection means.

摘要翻译： 提供表面等离子体场增强荧光光谱测量方法和表面等离子体激元增强荧光光谱测量装置，即使当分析物的浓度高时，也能够精确地测量荧光信号而不管光检测装置的类型 通过调整SPFS设备的动态范围。 一种表面等离子体场增强荧光立体测定方法，其中用荧光物质标记的分析物通过将激发光施加到金属薄膜而产生的表面等离子体激元激发，并且由光检测装置接收产生的荧光，从而检测分析物 。 通过调节由光检测装置接收的荧光的量来扩大动态范围。

公开(公告)号：US09551662B2

公开(公告)日：2017-01-24

申请号：US14118341

申请日：2012-04-23

申请人： Takatoshi Kaya

发明人： Takatoshi Kaya

IPC分类号： G01N21/64

CPC分类号： G01N21/6428 ,  G01N21/6452 ,  G01N21/648

摘要： A surface plasmon-field enhanced fluorescence measurement device may be provided to accurately detect a specific substance even in the case in which a well member is used and achieving a simpler structure and a lower manufacturing cost, and a fluorescence detection method using the surface plasmon-field enhanced fluorescence measurement device.

摘要翻译： 也可以提供表面等离子体场增强型荧光测定装置，即使在使用了阱部件的情况下也能够精确地检测特定物质，能够实现更简单的结构和更低的制造成本，并且使用表面等离子体激元 场增强荧光测量装置。

公开(公告)号：US20140061506A1

公开(公告)日：2014-03-06

申请号：US14118341

申请日：2012-04-23

申请人： Takatoshi Kaya

发明人： Takatoshi Kaya

IPC分类号： G01N21/64

CPC分类号： G01N21/6428 ,  G01N21/6452 ,  G01N21/648

摘要： A surface plasmon-field enhanced fluorescence measurement device may be provided to accurately detect a specific substance even in the case in which a well member is used and achieving a simpler structure and a lower manufacturing cost, and a fluorescence detection method using the surface plasmon-field enhanced fluorescence measurement device.

摘要翻译： 即使在使用了阱构件的情况下也可以提供表面等离子体场增强型荧光测定装置，能够精确地检测特定物质，并且能够实现更简单的结构和更低的制造成本，并且使用表面等离子体激元荧光检测装置， 场增强荧光测量装置。

公开(公告)号：US09068945B2

公开(公告)日：2015-06-30

申请号：US14126752

申请日：2012-05-31

申请人： Naoki Hikage ,  Masataka Matsuo ,  Takatoshi Kaya

发明人： Naoki Hikage ,  Masataka Matsuo ,  Takatoshi Kaya

IPC分类号： G01N21/64 ,  G01N33/543 ,  B01L3/00 ,  G01N33/58

CPC分类号： G01N21/64 ,  B01L3/502715 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/168 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/582 ,  G01N2021/6417 ,  G01N2021/6439 ,  G01N2021/6482

摘要： To provide a surface plasmon-field enhanced fluorescence spectroscopic measurement method and a surface plasmon-field enhanced fluorescence spectroscopic measurement device which are capable of accurately measuring a fluorescent signal regardless of the type of a light detection means even when the concentration of an analyte is high by adjusting the dynamic range of the SPFS device. A surface plasmon-field enhanced fluorescence stereoscopic measurement method wherein an analyte labeled with a fluorescent substance is excited by surface plasmon light generated by applying excitation light to a metallic thin film, and generated fluorescence is received by a light detection means to thereby detect the analyte. The dynamic range is expanded by adjusting the amount of the fluorescence received by the light detection means.

摘要翻译： 提供表面等离子体场增强荧光光谱测量方法和表面等离子体激元增强荧光光谱测量装置，即使当分析物的浓度高时，也能够精确地测量荧光信号而不管光检测装置的类型 通过调整SPFS设备的动态范围。 一种表面等离子体场增强荧光立体测定方法，其中用荧光物质标记的分析物通过将激发光施加到金属薄膜而产生的表面等离子体激元激发，并且由光检测装置接收产生的荧光，从而检测分析物 。 通过调节由光检测装置接收的荧光的量来扩大动态范围。