公开(公告)号：US20130078148A1

公开(公告)日：2013-03-28

申请号：US13702459

申请日：2011-06-06

申请人： Takatoshi Kaya ,  Naoki Hikage ,  Hidetaka Ninomiya

发明人： Takatoshi Kaya ,  Naoki Hikage ,  Hidetaka Ninomiya

IPC分类号： G01N21/64

CPC分类号： G01N21/64 ,  C03C17/40 ,  G01N21/6428 ,  G01N21/648 ,  G01N33/54373 ,  G01N2021/6439 ,  G01N2610/00

摘要： A surface plasmon-field enhanced fluorescence spectroscopy [SPFS] sensor chip may include a transparent support, a metal thin film formed on one surface of the transparent support, a self-assembled monolayer [SAM] formed on a surface of the metal thin film, said surface not being in contact with the transparent support, a solid phase layer formed on a surface of the SAM and having a three-dimensional structure, said surface not being in contact with the metal thin film, and a ligand immobilized in the solid phase layer. A fluctuation ratio represented by the following formula is not less than 0% but not more than 30%: {half-width (α)−half-width (β)}/half-width (β)×100.

摘要翻译： 表面等离子体场增强荧光光谱[SPFS]传感器芯片可以包括透明支撑体，形成在透明支撑体的一个表面上的金属薄膜，在金属薄膜的表面上形成的自组装单层[SAM] 所述表面不与透明支撑体接触，形成在SAM表面上并具有三维结构的固相层，所述表面不与金属薄膜接触，并且固定在固相中的配体 层。 由下式表示的波动比不小于0％但不大于30％：{半宽（α） - 半峰宽（＆bgr）} /半宽（＆bgr）×100。

公开(公告)号：US09068945B2

公开(公告)日：2015-06-30

申请号：US14126752

申请日：2012-05-31

申请人： Naoki Hikage ,  Masataka Matsuo ,  Takatoshi Kaya

发明人： Naoki Hikage ,  Masataka Matsuo ,  Takatoshi Kaya

IPC分类号： G01N21/64 ,  G01N33/543 ,  B01L3/00 ,  G01N33/58

CPC分类号： G01N21/64 ,  B01L3/502715 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/168 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/582 ,  G01N2021/6417 ,  G01N2021/6439 ,  G01N2021/6482

摘要： To provide a surface plasmon-field enhanced fluorescence spectroscopic measurement method and a surface plasmon-field enhanced fluorescence spectroscopic measurement device which are capable of accurately measuring a fluorescent signal regardless of the type of a light detection means even when the concentration of an analyte is high by adjusting the dynamic range of the SPFS device. A surface plasmon-field enhanced fluorescence stereoscopic measurement method wherein an analyte labeled with a fluorescent substance is excited by surface plasmon light generated by applying excitation light to a metallic thin film, and generated fluorescence is received by a light detection means to thereby detect the analyte. The dynamic range is expanded by adjusting the amount of the fluorescence received by the light detection means.

摘要翻译： 提供表面等离子体场增强荧光光谱测量方法和表面等离子体激元增强荧光光谱测量装置，即使当分析物的浓度高时，也能够精确地测量荧光信号而不管光检测装置的类型 通过调整SPFS设备的动态范围。 一种表面等离子体场增强荧光立体测定方法，其中用荧光物质标记的分析物通过将激发光施加到金属薄膜而产生的表面等离子体激元激发，并且由光检测装置接收产生的荧光，从而检测分析物 。 通过调节由光检测装置接收的荧光的量来扩大动态范围。

公开(公告)号：US20140117255A1

公开(公告)日：2014-05-01

申请号：US14126752

申请日：2012-05-31

申请人： Naoki Hikage ,  Masataka Matsuo ,  Takatoshi Kaya

发明人： Naoki Hikage ,  Masataka Matsuo ,  Takatoshi Kaya

IPC分类号： G01N21/64

CPC分类号： G01N21/64 ,  B01L3/502715 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/168 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/582 ,  G01N2021/6417 ,  G01N2021/6439 ,  G01N2021/6482

摘要： To provide a surface plasmon-field enhanced fluorescence spectroscopic measurement method and a surface plasmon-field enhanced fluorescence spectroscopic measurement device which are capable of accurately measuring a fluorescent signal regardless of the type of a light detection means even when the concentration of an analyte is high by adjusting the dynamic range of the SPFS device. A surface plasmon-field enhanced fluorescence stereoscopic measurement method wherein an analyte labeled with a fluorescent substance is excited by surface plasmon light generated by applying excitation light to a metallic thin film, and generated fluorescence is received by a light detection means to thereby detect the analyte. The dynamic range is expanded by adjusting the amount of the fluorescence received by the light detection means.

摘要翻译： 提供表面等离子体场增强荧光光谱测量方法和表面等离子体激元增强荧光光谱测量装置，即使当分析物的浓度高时，也能够精确地测量荧光信号而不管光检测装置的类型 通过调整SPFS设备的动态范围。 一种表面等离子体场增强荧光立体测定方法，其中用荧光物质标记的分析物通过将激发光施加到金属薄膜而产生的表面等离子体激元激发，并且由光检测装置接收产生的荧光，从而检测分析物 。 通过调节由光检测装置接收的荧光的量来扩大动态范围。

公开(公告)号：US20120156800A1

公开(公告)日：2012-06-21

申请号：US13393020

申请日：2010-09-03

申请人： Youichi Aoki ,  Kusunoki Higashino ,  Naoki Hikage

发明人： Youichi Aoki ,  Kusunoki Higashino ,  Naoki Hikage

IPC分类号： G01N1/28 ,  G01N21/55 ,  G01N21/64 ,  B01L3/00

CPC分类号： B01L3/50273 ,  B01L2200/0684 ,  B01L2200/143 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0877 ,  G01N21/553 ,  G01N21/648 ,  G01N33/54373 ,  G01N2035/00158 ,  G01N2035/106 ,  Y10T436/2575

摘要： A liquid feeding system for a microchip performs: a first liquid feeding step in which a sample liquid in a sample liquid containing section is fed in the direction to a primary containing section via a reaction field; a second liquid feeding step in which, after the first liquid feeding step, the sample liquid is fed from the primary containing section in the direction to the reaction field; and a third liquid feeding step in which, after the second liquid feeding step, the feedings of the sample liquid from and to the reaction field and the primary containing section a rear side gas-liquid boundary face of the sample liquid in the first liquid feeding step and the front side and rear side gas-liquid boundary faces of the sample liquid in the second and third liquid feeding steps do not pass through the reaction field.

摘要翻译： 用于微芯片的液体供给系统执行：第一液体供给步骤，其中样品液体容纳部分中的样品液体经由反应场沿着方向被供给到初级容纳部分; 第二液体供给步骤，在第一供液步骤之后，将样品液体从主容纳部分沿着反应场方向进料; 以及第三液体供给步骤，其中在所述第二供液步骤之后，所述样品液体从所述反应场和所述主容纳部分进料到所述第一液体进料中的样品液体的后侧气液界面 第二和第三液体供给步骤中的样品液体的前侧和后侧气液界面不通过反应场。

公开(公告)号：US09255883B2

公开(公告)日：2016-02-09

申请号：US13499760

申请日：2010-09-25

申请人： Masataka Matsuo ,  Yukito Nakamura ,  Naoki Hikage

发明人： Masataka Matsuo ,  Yukito Nakamura ,  Naoki Hikage

IPC分类号： G02B26/10 ,  G02B26/00 ,  G01N21/55 ,  G01N21/552 ,  G01N21/64 ,  G01N21/89

CPC分类号： G01N21/648 ,  G01N21/55 ,  G01N21/553 ,  G02B26/00 ,  G02B26/10

摘要： Provided is an apparatus having a beam geometry changing device that changes the beam geometry of excitation light, and a control device that controls the beam geometry changing device, with favorable precision, which the apparatus performs a resonance angle scan and to detect a target material.

摘要翻译： 提供了具有改变激发光的光束几何形状的光束几何形状改变装置的装置，以及以良好精度控制光束几何形状改变装置的控制装置，该装置执行共振角度扫描并检测目标材料。

公开(公告)号：US09375713B2

公开(公告)日：2016-06-28

申请号：US13393020

申请日：2010-09-03

申请人： Youichi Aoki ,  Kusunoki Higashino ,  Naoki Hikage

发明人： Youichi Aoki ,  Kusunoki Higashino ,  Naoki Hikage

IPC分类号： G01N1/28 ,  G01N21/55 ,  G01N21/64 ,  B01L3/00 ,  G01N21/552 ,  G01N33/543 ,  G01N35/00 ,  G01N35/10

CPC分类号： B01L3/50273 ,  B01L2200/0684 ,  B01L2200/143 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0877 ,  G01N21/553 ,  G01N21/648 ,  G01N33/54373 ,  G01N2035/00158 ,  G01N2035/106 ,  Y10T436/2575

摘要： A liquid feeding system for a microchip performs: a first liquid feeding step in which a sample liquid in a sample liquid containing section is fed in the direction to a primary containing section via a reaction field; a second liquid feeding step in which, after the first liquid feeding step, the sample liquid is fed from the primary containing section in the direction to the reaction field; and a third liquid feeding step in which, after the second liquid feeding step, the feedings of the sample liquid from and to the reaction field and the primary containing section a rear side gas-liquid boundary face of the sample liquid in the first liquid feeding step and the front side and rear side gas-liquid boundary faces of the sample liquid in the second and third liquid feeding steps do not pass through the reaction field.

摘要翻译： 用于微芯片的液体供给系统执行：第一液体供给步骤，其中样品液体容纳部分中的样品液体经由反应场沿着方向被供给到初级容纳部分; 第二液体供给步骤，在第一供液步骤之后，将样品液体从主容纳部分沿着反应场方向进料; 以及第三液体供给步骤，其中在所述第二供液步骤之后，所述样品液体从所述反应场和所述主容纳部分进料到所述第一液体进料中的样品液体的后侧气液界面 第二和第三液体供给步骤中的样品液体的前侧和后侧气液界面不通过反应场。

公开(公告)号：US20120201716A1

公开(公告)日：2012-08-09

申请号：US13499760

申请日：2010-09-25

申请人： Masataka Matsuo ,  Yukito Nakamura ,  Naoki Hikage

发明人： Masataka Matsuo ,  Yukito Nakamura ,  Naoki Hikage

IPC分类号： G01N21/64

CPC分类号： G01N21/648 ,  G01N21/55 ,  G01N21/553 ,  G02B26/00 ,  G02B26/10

摘要： Provided is an apparatus having a beam geometry changing device that changes the beam geometry of excitation light, and a control device that controls the beam geometry changing device, with favorable precision, which the apparatus performs a resonance angle scan and to detect a target material.

摘要翻译： 提供了具有改变激发光的光束几何形状的光束几何形状改变装置的装置，以及以良好精度控制光束几何形状改变装置的控制装置，该装置执行共振角度扫描并检测目标材料。