公开(公告)号：US06174715B1

公开(公告)日：2001-01-16

申请号：US08873235

申请日：1997-06-11

申请人： Masayoshi Muramatsu ,  Ayumi Koike ,  Kyozo Ogura ,  Tanetoshi Koyama ,  Naoto Shimizu ,  Yenwin Cho

发明人： Masayoshi Muramatsu ,  Ayumi Koike ,  Kyozo Ogura ,  Tanetoshi Koyama ,  Naoto Shimizu ,  Yenwin Cho

IPC分类号： C12N910

CPC分类号： C12N9/1085 ,  Y10S435/832 ,  Y10S435/839

摘要： The present invention provides a polypeptide selected from a polypeptide of subunit (A) or a polypeptide of subunit (B) of a prenyl diphosphate synthetase; a DNA coding for the polypeptide; a recombinant vector comprising the DNA; a transformant transformed with the vector; and a method for preparing an active type prenyl diphosphate synthetase. Also disclosed is a method for preparing an active type enzyme on which a specific property has been conferred, comprising mixing polypeptides of the two subunits of a heterodimeric enzyme, the polypeptides being derived from different organisms and one of the polypeptides having the specific property.

摘要翻译： 本发明提供了选自亚基（A）的多肽或异戊二烯基二磷酸合成酶的亚基（B）的多肽的多肽; 编码该多肽的DNA; 包含该DNA的重组载体; 用载体转化的转化体 以及制备活性型异戊二烯基二磷酸合成酶的方法。 还公开了一种制备其上赋予特异性质的活性型酶的方法，其包括混合异二聚体酶的两个亚基的多肽，所述多肽来源于不同生物体和具有特定性质的多肽之一。

公开(公告)号：US6071733A

公开(公告)日：2000-06-06

申请号：US217609

申请日：1998-12-22

申请人： Masayoshi Muramatsu ,  Ayumi Koike ,  Kyozo Ogura ,  Tanetoshi Koyama ,  Naoto Shimizu ,  Yenwin Cho

发明人： Masayoshi Muramatsu ,  Ayumi Koike ,  Kyozo Ogura ,  Tanetoshi Koyama ,  Naoto Shimizu ,  Yenwin Cho

IPC分类号： C12N9/10 ,  C12N15/54 ,  C07H21/04 ,  C12N1/00 ,  C12N1/20

CPC分类号： C12N9/1085 ,  Y10S435/832 ,  Y10S435/839

摘要： The present invention provides a polypeptide selected from a polypeptide of subunit (A) or a polypeptide of subunit (B) of a prenyl diphosphate synthetase; a DNA coding for the polypeptide; a recombinant vector comprising the DNA; a transformant transformed with the vector; and a method for preparing an active type prenyl diphosphate synthetase. Also disclosed is a method for preparing an active type enzyme on which a specific property has been conferred, comprising mixing polypeptides of the two subunits of a heterodimeric enzyme, the polypeptides being derived from different organisms and one of the polypeptides having the specific property.

摘要翻译： 本发明提供了选自亚基（A）的多肽或异戊二烯基二磷酸合成酶的亚基（B）的多肽的多肽; 编码该多肽的DNA; 包含该DNA的重组载体; 用载体转化的转化体 以及制备活性型异戊二烯基二磷酸合成酶的方法。 还公开了一种制备其上赋予特异性质的活性型酶的方法，其包括混合异二聚体酶的两个亚基的多肽，所述多肽来源于不同生物体和具有特定性质的多肽之一。