公开(公告)号：US20120015073A1

公开(公告)日：2012-01-19

申请号：US13260249

申请日：2010-03-26

申请人： Masayuki Kamijo

发明人： Masayuki Kamijo

IPC分类号： A23C9/123 ,  C12N1/20

CPC分类号： A23C9/1234 ,  A23Y2220/00 ,  A23Y2220/37 ,  A23Y2300/41 ,  C12N1/20

摘要： First, prepared is a whey degradation medium to which a protease, yeast extract, and the like are added. Further, polyoxyethylene sorbitan monooleate or propylene glycol monooleate is added to the whey degradation medium. The whey degradation medium is inoculated with bacteriocin-producing lactic acid bacterium and the lactic acid bacterium is cultured while the whey degradation medium is maintained at pH of 4 to 5. After the completion of the culture, the whey degradation medium (culture solution) is centrifuged to thereby separate therefrom a concentrated cell suspension containing the lactic acid bacterium in a concentrated form. The concentrated cell suspension has very low antibacterial activity (several tens AU or less). Then, by adding the concentrated cell suspension to a yogurt mix and fermenting the same, it is possible to produce yogurt containing the bacteriocin-producing lactic acid bacterium without any delay in the fermentation.

摘要翻译： 首先，制备添加有蛋白酶，酵母提取物等的乳清分解培养基。 此外，将聚氧乙烯山梨糖醇酐单油酸酯或丙二醇单油酸酯加入到乳清降解介质中。 用乳杆菌产生乳酸菌接种乳清分解培养基，培养乳酸菌，同时将乳清分解培养基保持在pH4〜5。培养结束后，将乳清分解培养基（培养液） 离心，从而将含有浓缩形式的乳酸菌的浓缩细胞悬浮液分离。 浓缩的细胞悬浮液具有非常低的抗菌活性（几十AU或更少）。 然后，通过将浓缩的细胞悬浮液加入到酸奶混合物中并发酵，可以在发酵中没有任何延迟地生产含有产生细菌素的乳酸菌的酸奶。

公开(公告)号：US09426999B2

公开(公告)日：2016-08-30

申请号：US13260249

申请日：2010-03-26

申请人： Masayuki Kamijo

发明人： Masayuki Kamijo

IPC分类号： C12P7/56 ,  C12N1/20 ,  A23C9/123

CPC分类号： A23C9/1234 ,  A23Y2220/00 ,  A23Y2220/37 ,  A23Y2300/41 ,  C12N1/20

摘要： First, prepared is a whey degradation medium to which a protease, yeast extract, and the like are added. Further, polyoxyethylene sorbitan monooleate or propylene glycol monooleate is added to the whey degradation medium. The whey degradation medium is inoculated with bacteriocin-producing lactic acid bacterium and the lactic acid bacterium is cultured while the whey degradation medium is maintained at pH of 4 to 5. After the completion of the culture, the whey degradation medium (culture solution) is centrifuged to thereby separate therefrom a concentrated cell suspension containing the lactic acid bacterium in a concentrated form. The concentrated cell suspension has very low antibacterial activity (several tens AU or less). Then, by adding the concentrated cell suspension to a yogurt mix and fermenting the same, it is possible to produce yogurt containing the bacteriocin-producing lactic acid bacterium without any delay in the fermentation.

摘要翻译： 首先，制备添加有蛋白酶，酵母提取物等的乳清分解培养基。 此外，将聚氧乙烯山梨糖醇酐单油酸酯或丙二醇单油酸酯加入到乳清降解介质中。 用乳杆菌产生乳酸菌接种乳清分解培养基，培养乳酸菌，同时将乳清分解培养基保持在pH4〜5。培养结束后，将乳清分解培养基（培养液） 离心，从而将含有浓缩形式的乳酸菌的浓缩细胞悬浮液分离。 浓缩的细胞悬浮液具有非常低的抗菌活性（几十AU或更少）。 然后，通过将浓缩的细胞悬浮液加入到酸奶混合物中并发酵，可以在发酵中没有任何延迟地生产含有产生细菌素的乳酸菌的酸奶。

公开(公告)号：US08709516B2

公开(公告)日：2014-04-29

申请号：US13260254

申请日：2010-03-26

申请人： Kakuhei Isawa ,  Masayuki Kamijo

发明人： Kakuhei Isawa ,  Masayuki Kamijo

IPC分类号： A23C9/12

CPC分类号： A23C9/1234 ,  A23L3/3463 ,  A23L3/34635 ,  A23Y2220/37 ,  C12N1/20 ,  C12N1/38

摘要： A method for culturing lactic acid bacteria includes (a) adding a proteolytic enzyme to an aqueous whey solution containing whey and water to thereby prepare a culture solution containing whey degraded by the proteolytic enzyme; (b) inoculating the culture solution with Lactobacillus gasseri which is a bacteriocin-producing lactic acid bacteria; and (c) maintaining the culture solution inoculated with the bacteriocin-producing lactic acid bacteria at a pH of not lower than 5.2 and not higher than 5.8 to provide cultured lactic acid bacteria. After the completion of culturing, the culture solution is centrifuged to separate a concentrated cell suspension containing the lactic acid bacterium in a concentrated form. The concentrated cell suspension has extremely high antibacterial activity, several tens of thousands AU, and is usable as a food preservative. Yogurt is produced by fermenting a yogurt mix to which 0.01% to 0.1% by weight of the concentrated cell suspension is added.

摘要翻译： 培养乳酸菌的方法包括（a）将蛋白水解酶加入到含有乳清和水的乳清溶液中，从而制备含有蛋白水解酶降解的乳清的培养液; （b）用作为细菌素的乳酸菌的加氏乳杆菌接种培养液; 和（c）将生殖细菌素乳酸菌接种的培养液维持在不低于5.2且不高于5.8的pH下，以提供培养的乳酸菌。 培养完成后，将培养液离心分离浓缩形式的含有乳酸菌的浓缩细胞悬浮液。 浓缩细胞悬浮液具有极高的抗菌活性，几万AU，可用作食品防腐剂。 通过发酵加入浓缩的细胞悬浮液的0.01％至0.1％的酸奶混合物来生产酸奶。