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    Method for culturing lactic acid bacterium and method for producing fermented milk
    1.
    发明授权
    Method for culturing lactic acid bacterium and method for producing fermented milk 有权
    培养乳酸菌的方法及发酵乳的制造方法

    公开(公告)号：US09426999B2

    公开(公告)日：2016-08-30

    申请号：US13260249

    申请日：2010-03-26

    申请人： Masayuki Kamijo

    发明人： Masayuki Kamijo

    IPC分类号： C12P7/56 ,  C12N1/20 ,  A23C9/123

    CPC分类号： A23C9/1234 ,  A23Y2220/00 ,  A23Y2220/37 ,  A23Y2300/41 ,  C12N1/20

    摘要： First, prepared is a whey degradation medium to which a protease, yeast extract, and the like are added. Further, polyoxyethylene sorbitan monooleate or propylene glycol monooleate is added to the whey degradation medium. The whey degradation medium is inoculated with bacteriocin-producing lactic acid bacterium and the lactic acid bacterium is cultured while the whey degradation medium is maintained at pH of 4 to 5. After the completion of the culture, the whey degradation medium (culture solution) is centrifuged to thereby separate therefrom a concentrated cell suspension containing the lactic acid bacterium in a concentrated form. The concentrated cell suspension has very low antibacterial activity (several tens AU or less). Then, by adding the concentrated cell suspension to a yogurt mix and fermenting the same, it is possible to produce yogurt containing the bacteriocin-producing lactic acid bacterium without any delay in the fermentation.

    摘要翻译： 首先，制备添加有蛋白酶，酵母提取物等的乳清分解培养基。 此外，将聚氧乙烯山梨糖醇酐单油酸酯或丙二醇单油酸酯加入到乳清降解介质中。 用乳杆菌产生乳酸菌接种乳清分解培养基，培养乳酸菌，同时将乳清分解培养基保持在pH4〜5。培养结束后，将乳清分解培养基（培养液） 离心，从而将含有浓缩形式的乳酸菌的浓缩细胞悬浮液分离。 浓缩的细胞悬浮液具有非常低的抗菌活性（几十AU或更少）。 然后，通过将浓缩的细胞悬浮液加入到酸奶混合物中并发酵，可以在发酵中没有任何延迟地生产含有产生细菌素的乳酸菌的酸奶。

    METHOD FOR CULTURING LACTIC ACID BACTERIUM AND METHOD FOR PRODUCING FERMENTED MILK
    3.
    发明申请
    METHOD FOR CULTURING LACTIC ACID BACTERIUM AND METHOD FOR PRODUCING FERMENTED MILK 有权
    培养乳酸菌的方法和生产发酵乳的方法

    公开(公告)号：US20120015073A1

    公开(公告)日：2012-01-19

    申请号：US13260249

    申请日：2010-03-26

    申请人： Masayuki Kamijo

    发明人： Masayuki Kamijo

    IPC分类号： A23C9/123 ,  C12N1/20

    CPC分类号： A23C9/1234 ,  A23Y2220/00 ,  A23Y2220/37 ,  A23Y2300/41 ,  C12N1/20

    摘要： First, prepared is a whey degradation medium to which a protease, yeast extract, and the like are added. Further, polyoxyethylene sorbitan monooleate or propylene glycol monooleate is added to the whey degradation medium. The whey degradation medium is inoculated with bacteriocin-producing lactic acid bacterium and the lactic acid bacterium is cultured while the whey degradation medium is maintained at pH of 4 to 5. After the completion of the culture, the whey degradation medium (culture solution) is centrifuged to thereby separate therefrom a concentrated cell suspension containing the lactic acid bacterium in a concentrated form. The concentrated cell suspension has very low antibacterial activity (several tens AU or less). Then, by adding the concentrated cell suspension to a yogurt mix and fermenting the same, it is possible to produce yogurt containing the bacteriocin-producing lactic acid bacterium without any delay in the fermentation.

    摘要翻译： 首先，制备添加有蛋白酶，酵母提取物等的乳清分解培养基。 此外，将聚氧乙烯山梨糖醇酐单油酸酯或丙二醇单油酸酯加入到乳清降解介质中。 用乳杆菌产生乳酸菌接种乳清分解培养基，培养乳酸菌，同时将乳清分解培养基保持在pH4〜5。培养结束后，将乳清分解培养基（培养液） 离心，从而将含有浓缩形式的乳酸菌的浓缩细胞悬浮液分离。 浓缩的细胞悬浮液具有非常低的抗菌活性（几十AU或更少）。 然后，通过将浓缩的细胞悬浮液加入到酸奶混合物中并发酵，可以在发酵中没有任何延迟地生产含有产生细菌素的乳酸菌的酸奶。