公开(公告)号：US20160083722A1

公开(公告)日：2016-03-24

申请号：US14838409

申请日：2015-08-28

Applicant: Massachusetts Institute of Technology

Inventor： Eric M. Young ,  David Benjamin Gordon ,  Christopher Voigt

IPC: C12N15/10 ,  C12N15/113

CPC classification number: C12N15/1082 ,  C12N15/1093 ,  C12N15/113 ,  C12N15/81 ,  C40B40/06 ,  C40B40/08

Abstract: Expression cassettes comprising promoter and terminator combinations are provided and can be used to tune gene expression. Synthetic yeast promoters and methods of making them also are provided.

Abstract translation: 提供了包含启动子和终止子组合的表达盒，可用于调节基因表达。 还提供合成酵母启动子及其制备方法。

公开(公告)号：US20170159047A9

公开(公告)日：2017-06-08

申请号：US14838409

申请日：2015-08-28

Applicant: Massachusetts Institute of Technology

Inventor： Eric M. Young ,  David Benjamin Gordon ,  Christopher Voigt

IPC: C12N15/10 ,  C12N15/113

CPC classification number: C12N15/1082 ,  C12N15/1093 ,  C12N15/113 ,  C12N15/81 ,  C40B40/06 ,  C40B40/08

Abstract: Expression cassettes comprising promoter and terminator combinations are provided and can be used to tune gene expression. Synthetic yeast promoters and methods of making them also are provided.

公开(公告)号：US20180284125A1

公开(公告)日：2018-10-04

申请号：US15557447

申请日：2016-03-11

Applicant: THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： David Benjamin Gordon ,  Robert Nicol ,  Christopher Voigt ,  Yongjin Park ,  Graham N. Rockwell ,  Ritika Dwivedi

IPC: G01N33/68 ,  C12Q1/6874 ,  C12N15/10

Abstract: Disclosed are methods and compositions for labeling target molecules or associated target molecule tags with origin-specific nucleic acid barcodes. The identity, quantity, and/or activity of target molecules originating from particular discrete volumes, such as droplets, for example water-in-oil emulsions, can be determined by determining the sequence of the origin-specific nucleic acid barcodes (optionally in combination with additional barcodes, such as one or more additional nucleic acid and/or peptide barcode).