公开(公告)号：US20180291372A1

公开(公告)日：2018-10-11

申请号：US15573879

申请日：2016-05-13

Applicant: Massachusetts Institute of Technology

Inventor： Timothy Kuan-Ta Lu ,  Samuel David Perli ,  Hao Cui

IPC: C12N15/11 ,  A61K35/22 ,  C12N9/22 ,  C12N15/86

Abstract: The present disclosure is directed, in some embodiments, to engineered nucleic acids comprising a promoter operably linked to a nucleotide sequence encoding a guide ribonucleic acid (gRNA) that comprises a specificity determining sequence (SDS) and a protospacer adjacent motif (PAM). The present disclosure is directed, in some embodiments, to cells comprising, vectors comprising, and methods of producing the engineered nucleic acids.