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公开(公告)号：US20220091099A1
公开(公告)日：2022-03-24
申请号：US17404286
申请日：2021-08-17
IPC分类号： G01N33/50 , G01N33/487 , C12N15/87
摘要： Gene editing can be performed by introducing gene-editing components into a cell by mechanical cell disruption. Related apparatus, systems, techniques, and articles are also described.
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公开(公告)号：US12130281B2
公开(公告)日：2024-10-29
申请号：US17404286
申请日：2021-08-17
IPC分类号： G01N33/487 , C12N15/87 , G01N33/50 , C12Q1/00
CPC分类号： G01N33/5002 , C12N15/87 , G01N33/48721 , C12Q1/00
摘要： Gene editing can be performed by introducing gene-editing components into a cell by mechanical cell disruption. Related apparatus, systems, techniques, and articles are also described.
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公开(公告)号：US11125739B2
公开(公告)日：2021-09-21
申请号：US15542892
申请日：2016-01-12
IPC分类号： C12N15/87 , G01N33/50 , G01N33/487 , C12Q1/00
摘要： Gene editing can be performed by introducing gene-editing components into a cell by mechanical cell disruption. Related apparatus, systems, techniques, and articles are also described. The methods and systems of the invention solve the problem of intracellular delivery of gene editing components and gene editing complexes to target cells. The results described herein indicate that delivery of gene editing components, e.g., protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA), by mechanical disruption of cell membranes leads to successful gene editing. Because intracellular delivery of gene editing materials is a current challenge, the methods provide a robust mechanism to engineer target cells without the use of potentially harmful viral vectors or electric fields.
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