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    Transgenic plants displaying multiple virus resistance and a process for their production
    2.
    发明授权
    Transgenic plants displaying multiple virus resistance and a process for their production 失效
    显示出多种病毒抗性的转基因植物及其生产过程

    公开(公告)号：US5589625A

    公开(公告)日：1996-12-31

    申请号：US374229

    申请日：1995-01-18

    申请人： Mart Saarma ,  Merikke Kelve ,  Erkki Truve ,  Teemu Teeri

    发明人： Mart Saarma ,  Merikke Kelve ,  Erkki Truve ,  Teemu Teeri

    IPC分类号： C12N9/12 ,  C12N15/82 ,  A01H5/00

    CPC分类号： C12N15/8283 ,  C12N9/12

    摘要： This invention discloses transgenic plants, such as transgenic tobacco and potato, having resistance to multiple viral taxonomic groups using parts of the 2,5A oligoadenylate pathway. In particular, said plants are genetically engineered to contain a DNA sequence encoding at least one polypeptide having a 2,5A synthetase activity. By this means a step in the 2,5A oligoadenylate pathway heretofore believed to be missing in all plants is provided so that viral infection in the transgenic plants is inhibited via a 2,5A dependent endonuclease. Moreover, this invention relates to a process for the production of said transgenic plants by transfection with a genetically engineered DNA sequence encoding at least one polypeptide having a 2,5A synthetase activity.

    摘要翻译： 本发明公开了转基因植物，例如转基因烟草和马铃薯，其具有使用部分2,5A寡聚腺苷酸途径的多种病毒分类组的抗性。 特别地，所述植物被遗传工程化以含有编码至少一种具有2,5A合成酶活性的多肽的DNA序列。 通过这意味着提供迄今为止认为在所有植物中缺失的2,5A寡腺苷酸途径中的步骤，使得转基因植物中的病毒感染通过2,5A依赖性内切核酸酶被抑制。 此外，本发明涉及通过用编码具有2,5A合成酶活性的至少一种多肽的遗传工程改造的DNA序列转染来生产所述转基因植物的方法。

    Suppressors of RNA Silencing as Modulators of miRNA Levels
    3.
    发明申请
    Suppressors of RNA Silencing as Modulators of miRNA Levels 审中-公开
    RNA沉默抑制因子作为miRNA水平的调节剂

    公开(公告)号：US20130029417A1

    公开(公告)日：2013-01-31

    申请号：US13578703

    申请日：2011-02-18

    申请人： Maria Cecilia Sarmiento Guerin ,  Kairi Karblane ,  Illar Pata ,  Erkki Truve ,  Pille Pata

    发明人： Maria Cecilia Sarmiento Guerin ,  Kairi Karblane ,  Illar Pata ,  Erkki Truve ,  Pille Pata

    IPC分类号： C12N5/071

    CPC分类号： C07K14/415 ,  A61K38/162 ,  A61K48/005

    摘要： The invention describes use of RNA silencing suppressors or interactors of the suppressors to bring the expression of microRNAs involved in any disease, including malignant neoplasia, back to its normal level. More specifically the present invention provides a method to regulate many miRNAs at the same time. Most of the suppressors according to this invention are coded by plant viruses that unexpectedly can affect RNA silencing and modulate miRNA expression levels in mammalian cells. Also suppressors of endogenous origin are described as able to modulate miRNA expression levels.

    摘要翻译： 本发明描述了抑制剂的RNA沉默抑制剂或相互作用子的使用，使涉及任何疾病（包括恶性肿瘤）的微小RNA的表达恢复到其正常水平。 更具体地，本发明提供了同时调节许多miRNA的方法。 根据本发明的大多数抑制剂由植物病毒编码，其意外地可以影响哺乳动物细胞中的RNA沉默和调节miRNA表达水平。 内源起源的抑制因子也被描述为能够调节miRNA表达水平。