公开(公告)号：US20220064452A1

公开(公告)日：2022-03-03

申请号：US17410879

申请日：2021-08-24

申请人： Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e. V.

发明人： Michael WEBER ,  Stefan W. HELL ,  Alexey BUTKEVICH

IPC分类号： C09B11/24

摘要： Disclosed are photoactivable fluorescent dye compounds of formula I: wherein: n=0, 1, 2, 3; X is selected from O, CRR′, SiRR′ and GeRR′, where R and R′ represent independently alkyl, cycloalkyl, alkenyl, alkynyl or aryl; Y is H, SO3H or SO3M, with M being a positively charged counterion, in particular selected from NH4+ and cations of organic ammonium compounds; R1 is H, CO2H, C(O)NH-linker-CO2H, C(O)O-ligand, C(O)NH-ligand or C(O)NH-linker-ligand; R2 may represent H, unsubstituted or substituted alkyl (including cycloalkyl); R3 and R4 may represent independently H or F; R5 is H, Me, CO2H, C(O)NH-linker-CO2H, C(O)O-ligand, C(O)NH-ligand or C(O)NH-linker-ligand; wherein the ligand moiety at each occurrence represents a reactive group or tag, capable to form a covalent or non-covalent bond or molecular complex with a target chemical entity or substance. Methods of using the compounds in imaging of fixed and living cells are also disclosed.

公开(公告)号：US20180223102A1

公开(公告)日：2018-08-09

申请号：US15886494

申请日：2018-02-01

申请人： Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e. V.

发明人： Maksim SEDNEV ,  Alexey BUTKEVICH ,  Heydar SHOJAEI ,  Vladimir BELOV ,  Stefan HELL ,  Christian WURM ,  Dirk KAMIN

IPC分类号： C09B1/16 ,  C09B57/00 ,  C09B5/24

CPC分类号： C09B1/16 ,  C07F9/4021 ,  C07F9/4419 ,  C07F9/65522 ,  C07F9/655372 ,  C07F9/65586 ,  C07F9/6561 ,  C09B5/2436 ,  C09B57/00

摘要： Compounds of formula I are disclosed: wherein X1, X2, X3, X4 are independently H, F, Cl, Br, I, CN, NO2, OR1, SR1, NR1R2, COR1, COOR1, CONR1R2, PO3R1R2, SO2R1, SO3R1 or R3; R1 and R2 are, e.g., H, alkyl or aryl or optionally a ring; R3 is, e.g., alkyl, alkenyl, alkynyl, aryl or cycloalkyl; Y is OR1, NR1R2, or NR1R3; Q is O, S, SO2, NR, C(R3)2, Si(R3)2, Ge(R3)2, P(═O)R3 or P(═O)OR3; Q and X1 can optionally form part of a ring; L and M are independently OR1, SR1, NR1R2 and R3; L and M can optionally form part of a ring; Z is O, S, NR1, CR1R3 or aryl; and Z and X4 can optionally form part of a ring.