公开(公告)号：US20080213749A1

公开(公告)日：2008-09-04

申请号：US11713408

申请日：2007-03-02

申请人： Melanie Feola ,  Larisa Gofman ,  Eli Mordechai ,  Martin E. Adelson

发明人： Melanie Feola ,  Larisa Gofman ,  Eli Mordechai ,  Martin E. Adelson

IPC分类号： C07H21/00 ,  C12Q1/68 ,  C12Q1/70

CPC分类号： C12Q1/701

摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains a human metapneumovirus or has an increased likelihood of containing a human metapneumovirus, a virus which is a causative agent of respiratory tract disease in humans. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the fusion protein of a human metapneumovirus, are useful as forward and reverse primers for a polymerase chain reaction using reverse transcripts of RNA from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains a human metapneumovirus or has an increased likelihood of containing a human metapneumovirus.

摘要翻译： 公开了用于确定样品是否含有人类偏肺病毒或具有增加的可能性的可能性的寡核苷酸，所述可能性包含人类偏肺病毒，人类偏肺病毒是人类呼吸道疾病的致病因子的病毒。 具有源自编码人类偏肺病毒融合蛋白的基因的编码区的核苷酸序列的这些寡核苷酸可用作使用来自生物样品的RNA的逆转录作为模板的聚合酶链反应的正向引物和反向引物，以及 用于检测任何所得扩增子的探针。 扩增子的检测表明样品含有人类偏肺病毒，或具有增加含有人间质肺病毒的可能性。

公开(公告)号：US08932832B2

公开(公告)日：2015-01-13

申请号：US13199380

申请日：2011-08-26

申请人： Martin E. Adelson ,  Melanie Feola ,  Jason Trama ,  Eli Mordechai

发明人： Martin E. Adelson ,  Melanie Feola ,  Jason Trama ,  Eli Mordechai

IPC分类号： C07H21/04 ,  C12Q1/70

CPC分类号： C12Q1/705

摘要： Methods are described herein for detecting and identifying distinct species of nucleic acids, in a single container, for example, from a certain genus of infectious agents or otherwise causative agents comprising, for example, providing a forward PCR primer common to a homologous gene region between the distinct species, and providing a reverse PCR primer common to a homologous gene region between the distinct species, to thereby define a PCR target region amongst the species, and providing a first oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a first species, providing a second oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a second species, wherein the first and second oligonucleotide probes are each detectably labeled with distinctly different detectable labels, conducting a PCR reaction in the container by means of the primers to amplify the target region amongst the species, and detecting the distinct labels, thereby identifying distinct species of nucleic acids corresponding to distinct species of infectious agents. Methods are preferred, for example, wherein the infectious agent is a member of the Herpesviridae family.

摘要翻译： 在本文中描述了用于在单个容器中，例如来自某一感染因子或其他致病因子的单个容器中检测和鉴定不同种类的核酸的方法，所述诱导剂包括例如提供在同源基因区域之间共同的正向PCR引物 并且提供与不同物种之间的同源基因区域共有的反向PCR引物，从而在物种间限定PCR靶区域，并提供对靶区域内的核酸序列特异的第一寡核苷酸探针， 提供对目标区域内具有特征的第二种类的核酸序列特异性的第二寡核苷酸探针，其中第一和第二寡核苷酸探针各自可检测地用明显不同的可检测标记物标记，进行PCR反应 在容器中通过引物扩增目标区域amo 并检测不同的标签，从而识别与不同种类的感染因子相对应的不同种类的核酸。 方法是优选的，例如，其中感染剂是疱疹病毒科家族的成员。

公开(公告)号：US08030032B2

公开(公告)日：2011-10-04

申请号：US11246976

申请日：2005-10-07

申请人： Martin E. Adelson ,  Melanie Feola ,  Jason Trama ,  Eli Mordechai

发明人： Martin E. Adelson ,  Melanie Feola ,  Jason Trama ,  Eli Mordechai

IPC分类号： C12P19/34

CPC分类号： C12Q1/705

摘要： Methods are described herein for detecting and identifying distinct species of nucleic acids, in a single container, for example, from a certain genus of infectious agents or otherwise causative agents comprising, for example, providing a forward PCR primer common to a homologous gene region between the distinct species, and providing a reverse PCR primer common to a homologous gene region between the distinct species, to thereby define a PCR target region amongst the species, and providing a first oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a first species, providing a second oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a second species, wherein the first and second oligonucleotide probes are each detectably labeled with distinctly different detectable labels, conducting a PCR reaction in the container by means of the primers to amplify the target region amongst the species, and detecting the distinct labels, thereby identifying distinct species of nucleic acids corresponding to distinct species of infectious agents. Methods are preferred, for example, wherein the infectious agent is a member of the Herpesviridae family.

摘要翻译： 在本文中描述了用于在单个容器中，例如来自某一感染因子或其他致病因子的单个容器中检测和鉴定不同种类的核酸的方法，所述诱导剂包括例如提供在同源基因区域之间共同的正向PCR引物 并且提供与不同物种之间的同源基因区域共有的反向PCR引物，从而在物种间限定PCR靶区域，并提供对靶区域内的核酸序列特异的第一寡核苷酸探针， 提供对目标区域内具有特征的第二种类的核酸序列特异性的第二寡核苷酸探针，其中第一和第二寡核苷酸探针各自可检测地用明显不同的可检测标记物标记，进行PCR反应 在容器中通过引物扩增目标区域amo 并检测不同的标签，从而识别与不同种类的感染因子相对应的不同种类的核酸。 方法是优选的，例如，其中感染剂是疱疹病毒科家族的成员。

公开(公告)号：US20120064509A1

公开(公告)日：2012-03-15

申请号：US13199380

申请日：2011-08-26

申请人： Martin E. Adelson ,  Melanie Feola ,  Jason Trama ,  Eli Mordechai

发明人： Martin E. Adelson ,  Melanie Feola ,  Jason Trama ,  Eli Mordechai

IPC分类号： C12Q1/70

CPC分类号： C12Q1/705

摘要： Methods are described herein for detecting and identifying distinct species of nucleic acids, in a single container, for example, from a certain genus of infectious agents or otherwise causative agents comprising, for example, providing a forward PCR primer common to a homologous gene region between the distinct species, and providing a reverse PCR primer common to a homologous gene region between the distinct species, to thereby define a PCR target region amongst the species, and providing a first oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a first species, providing a second oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a second species, wherein the first and second oligonucleotide probes are each detectably labeled with distinctly different detectable labels, conducting a PCR reaction in the container by means of the primers to amplify the target region amongst the species, and detecting the distinct labels, thereby identifying distinct species of nucleic acids corresponding to distinct species of infectious agents. Methods are preferred, for example, wherein the infectious agent is a member of the Herpesviridae family.

摘要翻译： 在本文中描述了用于在单个容器中，例如来自某一感染因子或其他致病因子的单个容器中检测和鉴定不同种类的核酸的方法，所述诱导剂包括例如提供在同源基因区域之间共同的正向PCR引物 并且提供与不同物种之间的同源基因区域共有的反向PCR引物，从而在物种间限定PCR靶区域，并提供对靶区域内的核酸序列特异的第一寡核苷酸探针， 提供对目标区域内具有特征的第二种类的核酸序列特异性的第二寡核苷酸探针，其中第一和第二寡核苷酸探针各自可检测地用明显不同的可检测标记物标记，进行PCR反应 在容器中通过引物扩增目标区域amo 并检测不同的标签，从而识别与不同种类的感染因子相对应的不同种类的核酸。 方法是优选的，例如，其中感染剂是疱疹病毒科家族的成员。

公开(公告)号：US08206930B2

公开(公告)日：2012-06-26

申请号：US13199093

申请日：2011-08-18

申请人： Melanie Feola ,  Martin Adelson ,  Eli Mordechai ,  Lisa Novak

发明人： Melanie Feola ,  Martin Adelson ,  Eli Mordechai ,  Lisa Novak

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/689 ,  Y02A50/57

摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains Borrelia afzelii, a spirochete which is a causative agent of Lyme disease in humans. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the p24 gene for the outer surface protein of Borrelia afzelii, are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains Borrelia afzelii. Real-time PCR and detection using florescence resonance energy transfer is disclosed.

公开(公告)号：US08026056B2

公开(公告)日：2011-09-27

申请号：US12156029

申请日：2008-05-29

申请人： Melanie Feola ,  Martin Adelson ,  Eli Mordechai ,  Lisa Novak

发明人： Melanie Feola ,  Martin Adelson ,  Eli Mordechai ,  Lisa Novak

IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/689 ,  Y02A50/57

摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains Borrelia afzelii, a spirochete which is a causative agent of Lyme disease in humans. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the p24 gene for the outer surface protein of Borrelia afzelii, are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains Borrelia afzelii. Real-time PCR and detection using florescence resonance energy transfer is disclosed.

摘要翻译： 公开了用于确定样品是否含有人类的莱姆病的致病因子的螺旋体（Borrelia afzelii）的方法中的寡核苷酸。 这些寡核苷酸具有衍生自编码b24基因的基因的编码区段的核苷酸序列，用于使用来自生物样品的核酸作为模板的聚合酶链反应的正向和反向引物， 以及用于检测任何所得扩增子的探针。 扩增子的检测表明样品含有Borrelia afzelii。 公开了使用荧光共振能量转移的实时PCR和检测。

公开(公告)号：US20090275023A1

公开(公告)日：2009-11-05

申请号：US12156029

申请日：2008-05-29

申请人： Melanie Feola ,  Martin Adelson ,  Eli Mordechai ,  Lisa Novak

发明人： Melanie Feola ,  Martin Adelson ,  Eli Mordechai ,  Lisa Novak

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/689 ,  Y02A50/57

摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains Borrelia afzelii, a spirochete which is a causative agent of Lyme disease in humans. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the p24 gene for the outer surface protein of Borrelia afzelii, are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains Borrelia afzelii. Real-time PCR and detection using florescence resonance energy transfer is disclosed.

摘要翻译： 公开了用于确定样品是否含有人类的莱姆病的致病因子的螺旋体（Borrelia afzelii）的方法中的寡核苷酸。 这些寡核苷酸具有衍生自编码b24基因的基因的编码区段的核苷酸序列，用于使用来自生物样品的核酸作为模板的聚合酶链反应的正向和反向引物， 以及用于检测任何所得扩增子的探针。 扩增子的检测表明样品含有Borrelia afzelii。 公开了使用荧光共振能量转移的实时PCR和检测。

公开(公告)号：US20060088865A1

公开(公告)日：2006-04-27

申请号：US11246976

申请日：2005-10-07

申请人： Martin Adelson ,  Melanie Feola ,  Jason Trama ,  Eli Mordechai

发明人： Martin Adelson ,  Melanie Feola ,  Jason Trama ,  Eli Mordechai

IPC分类号： C12Q1/70 ,  C12Q1/68

CPC分类号： C12Q1/705

摘要： Methods are described herein for detecting and identifying distinct species of nucleic acids, in a single container, for example, from a certain genus of infectious agents or otherwise causative agents comprising, for example, providing a forward PCR primer common to a homologous gene region between the distinct species, and providing a reverse PCR primer common to a homologous gene region between the distinct species, to thereby define a PCR target region amongst the species, and providing a first oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a first species, providing a second oligonucleotide probe specific to a nucleic acid sequence within the target region that is characteristic of a second species, wherein the first and second oligonucleotide probes are each detectably labeled with distinctly different detectable labels, conducting a PCR reaction in the container by means of the primers to amplify the target region amongst the species, and detecting the distinct labels, thereby identifying distinct species of nucleic acids corresponding to distinct species of infectious agents. Methods are preferred, for example, wherein the infectious agent is a member of the Herpesviridae family.

公开(公告)号：US08377656B2

公开(公告)日：2013-02-19

申请号：US13135022

申请日：2011-06-23

申请人： Melanie Feola ,  John Entwistle ,  Martin Adelson ,  Eli Mordechai

发明人： Melanie Feola ,  John Entwistle ,  Martin Adelson ,  Eli Mordechai

IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6895 ,  C07H21/04 ,  C12Q2600/158

摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains Cryptococcus neoformans, a causative agent for human cryptococcosis. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the fungal specific transcription factor gene in Cryptococcus neoformans, are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains Cryptococcus neoformans. Real-time PCR and detection using florescence resonance energy transfer is disclosed.

公开(公告)号：US20120122092A1

公开(公告)日：2012-05-17

申请号：US13199093

申请日：2011-08-18

申请人： Melanie Feola ,  Martin Adelson ,  Eli Mordechai ,  Lisa Novak

发明人： Melanie Feola ,  Martin Adelson ,  Eli Mordechai ,  Lisa Novak

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689 ,  Y02A50/57

摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains Borrelia afzelii, a spirochete which is a causative agent of Lyme disease in humans. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the p24 gene for the outer surface protein of Borrelia afzelii, are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains Borrelia afzelii. Real-time PCR and detection using florescence resonance energy transfer is disclosed.

摘要翻译： 公开了用于确定样品是否含有人类的莱姆病的致病因子的螺旋体（Borrelia afzelii）的方法中使用的寡核苷酸。 这些寡核苷酸具有衍生自编码b24基因的基因的编码区段的核苷酸序列，用于使用来自生物样品的核酸作为模板的聚合酶链反应的正向和反向引物， 以及用于检测任何所得扩增子的探针。 扩增子的检测表明样品含有Borrelia afzelii。 公开了使用荧光共振能量转移的实时PCR和检测。