专利检索 ap:("Merck Patent GmbH") AND inv:"Laura Rhiel" 第 1 页

1.

发明授权
Methods for non-covalent Fc-domain-containing protein display on the surface of cells and methods of screening thereof 有权

公开(公告)号：US10676733B2

公开(公告)日：2020-06-09

申请号：US15522108

申请日：2015-10-27

申请人： Merck Patent GmbH

发明人： Laura Rhiel , Stefan Becker , Ralf Guenther , Bjoern Hock , Daniel Helman , Mira Toister-Achituv , Simon Krah

IPC分类号： C40B30/06 , C12N15/10 , C07K14/36 , C07K14/315 , C07K14/195 , C07K16/00 , C12N15/81

摘要： The present invention provides methods for the non-covalent surface display of proteins of interest (POI), in particular for Fc-domain containing proteins such as antibodies. The inventive method may be used to screen and select proteins of interest of a desired phenotype. The present invention further discloses polynucleotides and proteins and methods of producing the same, which may be used in carrying out the inventive method.

2.

发明申请
METHODS FOR NON-COVALENT FC-DOMAIN-CONTAINING PROTEIN DISPLAY ON THE SURFACE OF CELLS AND METHODS OF SCREENING THEREOF 审中-公开

公开(公告)号：US20180298374A1

公开(公告)日：2018-10-18

申请号：US15522108

申请日：2015-10-27

申请人： Merck Patent GmbH

发明人： Laura Rhiel , Stefan Becker , Ralf Guenther , Bjoern Hock , Daniel Helman , Mira Toister-Achituv , Simon Krah

IPC分类号： C12N15/10 , C07K14/195 , C07K16/00 , C12N15/81

摘要： The present invention provides methods for the non-covalent surface display of proteins of interest (POI), in particular for Fc-domain containing proteins such as antibodies. The inventive method may be used to screen and select proteins of interest of a desired phenotype. The present invention further discloses polynucleotides and proteins and methods of producing the same, which may be used in carrying out the inventive method.

3.

发明授权
Anti-TNFα antibodies with pH-dependent antigen binding for improved target clearence 有权

公开(公告)号：US10183994B2

公开(公告)日：2019-01-22

申请号：US15322893

申请日：2015-06-26

申请人： Merck Patent GmbH

发明人： Ralf Guenther , Stefan Becker , Laura Rhiel , Bjoern Hock , Christian Schroeter

IPC分类号： A61K39/395 , C07K16/00 , C07K16/24 , A61K47/48 , A61K39/00

摘要： The invention relates to anti-TNFα antibodies which are engineered to exhibit a pH-sensitive antigen binding. The invention is preferably directed to anti-TNFα antibody adalimumab (Humira®) or biologically active variants and fragments thereof, wherein the original adalimumab antibody or variant or fragment thereof is engineered by modifications of amino acid sequence within the variable regions. Specifically, the invention relates to adalimumab or biologically active variants or fragments thereof, wherein the CDR domains are modified by replacing one or more amino acid residues by histidine residues.

4.

发明授权
Method of producing secretable antibodies by expression in saccharomyces cerevisiae 有权

公开(公告)号：US10138477B2

公开(公告)日：2018-11-27

申请号：US14758856

申请日：2013-12-11

申请人： Merck Patent GmbH

发明人： Ralph Guenther , Bjoern Hock , Stefan Becker , Laura Rhiel

IPC分类号： C12N15/10 , C07K16/00

摘要： The invention relates to a method for the production and non-covalent surface display of antibodies and derived fragments as well as molecule libraries based thereon on the surface of S. cerevisiae cells. The non-covalent manner of the surface display renders possible the selection of specific variants by means of high throughput screening and the subsequent switchable secretion of the selected binding molecule into the culture supernatant for biochemical characterization.