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1.发明公开公开(公告)号：US20240343800A1
公开(公告)日：2024-10-17
申请号：US18436931
申请日：2024-02-08
申请人： Merck Patent GmbH
发明人： Brian Rabinovich , Jessica Dawson , Xueyuan Zhou , Stefan Becker , Yves Fomekong Nanfack , Christian Schroeter , Lars Toleikis , Achim Doerner , Bjorn Hock
CPC分类号： C07K16/2809 , A61P35/00 , C07K16/2803 , C07K2317/21 , C07K2317/31 , C07K2317/622 , C07K2317/92
摘要： This disclosure relates generally to proteins target tumor cell killing comprising: a polypeptide or complex of two or more polypeptides that specifically binds ROR1, and a polypeptide or complex of two or more polypeptides that specifically binds CD3. In some embodiments, the present application provides antibodies that specifically bind ROR1. In some embodiments the application also provides therapeutic methods for using such proteins in the treatment of a cancer.
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2.发明申请公开(公告)号：US20180298374A1
公开(公告)日：2018-10-18
申请号：US15522108
申请日：2015-10-27
申请人： Merck Patent GmbH
发明人： Laura Rhiel , Stefan Becker , Ralf Guenther , Bjoern Hock , Daniel Helman , Mira Toister-Achituv , Simon Krah
IPC分类号： C12N15/10 , C07K14/195 , C07K16/00 , C12N15/81
摘要： The present invention provides methods for the non-covalent surface display of proteins of interest (POI), in particular for Fc-domain containing proteins such as antibodies. The inventive method may be used to screen and select proteins of interest of a desired phenotype. The present invention further discloses polynucleotides and proteins and methods of producing the same, which may be used in carrying out the inventive method.
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公开(公告)号：US20140357514A1
公开(公告)日：2014-12-04
申请号：US14361358
申请日：2012-12-14
申请人： Merck Patent GmbH
发明人： Stefan Becker , Tim Heiseler , Alexander Maass , Harald Kolmar
IPC分类号： G01N33/543 , G01N33/554
CPC分类号： G01N33/54353 , C12N15/1037 , C12N15/1086 , C40B30/08 , G01N33/532 , G01N33/542 , G01N33/554
摘要： A generally applicable method for the selective covalent attachment of a reporter molecule to a replicating entity that allows one to obtain specific binders from a single round of library screening is disclosed. For example, selective biotinylation of phage particles and yeast cells displaying a binder to any given target can be achieved via application of a coupled enzyme reaction that includes a peroxidase, an oxidase and a catalase.
摘要翻译： 公开了一种用于将报告分子选择性共价连接到允许从单次文库筛选获得特异性结合物的复制实体的普遍适用的方法。 例如，可以通过应用包括过氧化物酶，氧化酶和过氧化氢酶的偶联酶反应来实现向任何给定靶标显示粘合剂的噬菌体颗粒和酵母细胞的选择性生物素化。
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公开(公告)号：US11001640B2
公开(公告)日：2021-05-11
申请号：US15523888
申请日：2015-10-21
申请人： Merck Patent GmbH
发明人： Stefan Becker , Bjoern Hock , Stefan Zielonka , Harald Kolmar , Martin Empting
IPC分类号： A61K39/00 , C07K16/30 , C07K16/00 , C12N15/81 , A61K39/395
摘要： The present invention provides a method for generating bispecific shark variable antibody domains (vNAR domains) and uses thereof. The present invention further provides fusion proteins comprising the inventive bispecific vNAR domains as well as polynucleotide libraries for use in the generation of the inventive bi-specific vNARs. Furthermore, the invention provides pharmaceutical compositions comprising the inventive bispecific vNARs or fusion proteins comprising bi-specific vNAR domains for use in the treatment of pathological conditions in an individual. The invention also provides kits of parts comprising the bispecific vNAR domains or fusion proteins.
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公开(公告)号：US10676733B2
公开(公告)日：2020-06-09
申请号：US15522108
申请日：2015-10-27
申请人： Merck Patent GmbH
发明人： Laura Rhiel , Stefan Becker , Ralf Guenther , Bjoern Hock , Daniel Helman , Mira Toister-Achituv , Simon Krah
IPC分类号： C40B30/06 , C12N15/10 , C07K14/36 , C07K14/315 , C07K14/195 , C07K16/00 , C12N15/81
摘要： The present invention provides methods for the non-covalent surface display of proteins of interest (POI), in particular for Fc-domain containing proteins such as antibodies. The inventive method may be used to screen and select proteins of interest of a desired phenotype. The present invention further discloses polynucleotides and proteins and methods of producing the same, which may be used in carrying out the inventive method.
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公开(公告)号：US10183994B2
公开(公告)日：2019-01-22
申请号：US15322893
申请日：2015-06-26
申请人： Merck Patent GmbH
发明人： Ralf Guenther , Stefan Becker , Laura Rhiel , Bjoern Hock , Christian Schroeter
IPC分类号： A61K39/395 , C07K16/00 , C07K16/24 , A61K47/48 , A61K39/00
摘要： The invention relates to anti-TNFα antibodies which are engineered to exhibit a pH-sensitive antigen binding. The invention is preferably directed to anti-TNFα antibody adalimumab (Humira®) or biologically active variants and fragments thereof, wherein the original adalimumab antibody or variant or fragment thereof is engineered by modifications of amino acid sequence within the variable regions. Specifically, the invention relates to adalimumab or biologically active variants or fragments thereof, wherein the CDR domains are modified by replacing one or more amino acid residues by histidine residues.
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公开(公告)号：US10138477B2
公开(公告)日：2018-11-27
申请号：US14758856
申请日：2013-12-11
申请人： Merck Patent GmbH
发明人： Ralph Guenther , Bjoern Hock , Stefan Becker , Laura Rhiel
摘要： The invention relates to a method for the production and non-covalent surface display of antibodies and derived fragments as well as molecule libraries based thereon on the surface of S. cerevisiae cells. The non-covalent manner of the surface display renders possible the selection of specific variants by means of high throughput screening and the subsequent switchable secretion of the selected binding molecule into the culture supernatant for biochemical characterization.
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公开(公告)号：US20180171020A1
公开(公告)日：2018-06-21
申请号：US15523888
申请日：2015-10-21
申请人： Merck Patent GmbH
发明人： Stefan Becker , Bjoern Hock , Stefan Zielonka , Harald Kolmar , Martin Empting
CPC分类号： C07K16/30 , A61K39/39558 , A61K2039/505 , C07K16/00 , C07K2317/24 , C07K2317/31 , C07K2317/565 , C07K2317/569 , C07K2317/92 , C07K2319/30 , C12N15/81
摘要： The present invention provides a method for generating bispecific shark variable antibody domains (vNAR domains) and uses thereof. The present invention further provides fusion proteins comprising the inventive bispecific vNAR domains as well as polynucleotide libraries for use in the generation of the inventive bi-specific vNARs. Furthermore, the invention provides pharmaceutical compositions comprising the inventive bispecific vNARs or fusion proteins comprising bi-specific vNAR domains for use in the treatment of pathological conditions in an individual. The invention also provides kits of parts comprising the bispecific vNAR domains or fusion proteins.
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公开(公告)号：US10982008B2
公开(公告)日：2021-04-20
申请号：US15521891
申请日：2015-12-04
申请人： Merck Patent GmbH
摘要： The invention provides for a domain-exchanged antibody comprising a light chain (LC) composed of VL-CH3, and a heavy chain (HC) comprising VH-CH3-CH2-CH3, wherein the VL-CH3 of the LC is dimerising with the VH-CH3 of the HC thereby forming a domain-exchanged LC/HC dimer comprising a CH3LC/CH3HC domain pair, and means and method for producing the same.
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公开(公告)号：US09733240B2
公开(公告)日：2017-08-15
申请号：US14361358
申请日：2012-12-14
申请人： Merck Patent GmbH
发明人： Stefan Becker , Tim Heiseler , Alexander Maass , Harald Kolmar
IPC分类号： C40B30/08 , G01N33/543 , C12N15/10 , G01N33/532 , G01N33/542 , G01N33/554
CPC分类号： G01N33/54353 , C12N15/1037 , C12N15/1086 , C40B30/08 , G01N33/532 , G01N33/542 , G01N33/554
摘要： A generally applicable method for the selective covalent attachment of a reporter molecule to a replicating entity that allows one to obtain specific binders from a single round of library screening is disclosed. For example, selective biotinylation of phage particles and yeast cells displaying a binder to any given target can be achieved via application of a coupled enzyme reaction that includes a peroxidase, an oxidase and a catalase.
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