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8 条记录 (耗时 0.037 秒)

    Aggregate-free urate oxidase for preparation of non-immunogenic polymer conjugates
    1.
    发明申请
    Aggregate-free urate oxidase for preparation of non-immunogenic polymer conjugates 有权
    用于制备非免疫原性聚合物共轭物的无集料的尿酸氧化酶

    公开(公告)号：US20080057048A1

    公开(公告)日：2008-03-06

    申请号：US11882750

    申请日：2007-08-03

    申请人： Merry Sherman ,  Mark Saifer ,  L. Williams ,  Michael Hershfield ,  Susan Kelly

    发明人： Merry Sherman ,  Mark Saifer ,  L. Williams ,  Michael Hershfield ,  Susan Kelly

    IPC分类号： A61K38/54 ,  A61P43/00 ,  C12N9/06 ,  C12N9/96

    CPC分类号： A61K47/48215 ,  A61K38/00 ,  A61K47/60 ,  C12N9/0046 ,  C12N9/96

    摘要： A naturally occurring or recombinant protein, especially a mutein of porcine urate oxidase (uricase), that is essentially free of large aggregates can be rendered substantially non-immunogenic by conjugation with a sufficiently small number of strands of polymer such that the bioactivity of the protein is essentially retained in the conjugate. Such conjugates are unusually well suited for treatment of chronic conditions because they are less likely to induce the formation of antibodies and/or accelerated clearance than are similar conjugates prepared from protein preparations containing traces of large aggregates.

    摘要翻译： 基本上不含大量聚集体的天然存在或重组的蛋白质，特别是猪尿酸氧化酶（尿酸酶）的突变蛋白质可通过与足够少数量的聚合物链共轭而使其基本上不具有免疫原性，使得蛋白质的生物活性 基本上保留在缀合物中。 这种缀合物非常适合用于治疗慢性病症，因为它们与由含有痕量的大聚集体的蛋白质制剂制备的类似共轭物相比不太可能诱导抗体形成和/或加速清除。

    Polymer conjugates of cytokines, chemokines, growth factors, polypeptide hormones and antagonists thereof with preserved receptor-binding activity
    3.
    发明申请
    Polymer conjugates of cytokines, chemokines, growth factors, polypeptide hormones and antagonists thereof with preserved receptor-binding activity 审中-公开
    具有保护的受体结合活性的细胞因子，趋化因子，生长因子，多肽激素及其拮抗剂的聚合物缀合物

    公开(公告)号：US20080058246A1

    公开(公告)日：2008-03-06

    申请号：US11727641

    申请日：2007-03-27

    申请人： Shyam Bhaskaran ,  Merry Sherman ,  Mark Saifer ,  L. Williams

    发明人： Shyam Bhaskaran ,  Merry Sherman ,  Mark Saifer ,  L. Williams

    IPC分类号： A61K38/00 ,  C07K16/00

    CPC分类号： A61K38/2013 ,  A61K38/1808 ,  A61K38/191 ,  A61K38/212 ,  A61K38/30 ,  A61K47/60 ,  C07K14/52 ,  C07K14/521

    摘要： Methods are provided for the synthesis of polymer conjugates of cytokines, chemokines, growth factors, polypeptide hormones and receptor-binding antagonists thereof, which conjugates retain unusually high receptor-binding activity. Preparation of polymer conjugates according to the methods of the present invention diminishes or avoids steric inhibition of receptor-ligand interactions that commonly results from the attachment of polymers to receptor-binding regions of cytokines, chemokines, growth factors and polypeptide hormones, as well as to agonistic and antagonistic analogs thereof. The invention also provides conjugates and compositions produced by such methods. The conjugates of the present invention retain a higher level of receptor-binding activity than those produced by traditional polymer coupling methods that are not targeted to avoid receptor-binding domains of cytokines, chemokines, growth factors and polypeptide hormones. The conjugates of the present invention also exhibit an extended half-life in vivo and in vitro compared to unconjugated cytokines, chemokines, growth factors and polypeptide hormones. The present invention also provides kits comprising such conjugates and/or compositions, and methods of use of such conjugates and compositions in a variety of diagnostic, prophylactic, therapeutic and bioprocessing applications.

    摘要翻译： 提供了用于合成细胞因子，趋化因子，生长因子，多肽激素及其受体结合拮抗剂的聚合物缀合物的方法，该共轭物保留异常高的受体结合活性。 根据本发明的方法制备聚合物缀合物减少或避免了通常由聚合物与细胞因子，趋化因子，生长因子和多肽激素的受体结合区附着而引起的受体 - 配体相互作用的空间抑制，以及 其激动和拮抗类似物。 本发明还提供了通过这些方法制备的缀合物和组合物。 本发明的缀合物比没有靶向避免细胞因子，趋化因子，生长因子和多肽激素的受体结合结构域的传统聚合物偶联方法所产生的那些具有更高水平的受体结合活性。 与未结合的细胞因子，趋化因子，生长因子和多肽激素相比，本发明的缀合物在体内和体外也表现出延长的半衰期。 本发明还提供包含这种缀合物和/或组合物的试剂盒，以及在各种诊断，预防，治疗和生物加工应用中使用这种缀合物和组合物的方法。

    Aggregate-free protein compositions and methods of preparing same
    4.
    发明申请
    Aggregate-free protein compositions and methods of preparing same 审中-公开
    无蛋白组合物及其制备方法

    公开(公告)号：US20050014240A1

    公开(公告)日：2005-01-20

    申请号：US10928370

    申请日：2004-08-30

    申请人： Merry Sherman ,  Mark Saifer ,  L. Williams

    发明人： Merry Sherman ,  Mark Saifer ,  L. Williams

    IPC分类号： A61K38/00 ,  A61K38/44 ,  A61K47/48 ,  C12N1/21 ,  C12N9/02 ,  C12N9/06 ,  C12N9/96 ,  C07H21/04 ,  C12N5/06

    CPC分类号： C12N9/0048 ,  A61K38/00 ,  A61K47/60 ,  C12N9/0046 ,  C12N9/0093 ,  C12N9/96 ,  C12Y107/03003

    摘要： A naturally occurring or recombinant protein, especially a mutein of porcine urate oxidase (uricase), that is essentially free of large aggregates can be rendered substantially non-immunogenic by conjugation with a sufficiently small number of strands of polymer such that the bioactivity of the protein is essentially retained in the conjugate. Such conjugates are unusually well suited for treatment of chronic conditions because they are less likely to induce the formation of antibodies and/or accelerated clearance than are similar conjugates prepared from protein preparations containing traces of large aggregates.

    摘要翻译： 基本上不含大量聚集体的天然存在或重组的蛋白质，特别是猪尿酸氧化酶（尿酸酶）的突变蛋白质可通过与足够少数量的聚合物链共轭而使其基本上不具有免疫原性，使得蛋白质的生物活性 基本上保留在缀合物中。 这种缀合物非常适合用于治疗慢性病症，因为它们与由含有痕量的大聚集体的蛋白质制剂制备的类似共轭物相比不太可能诱导抗体形成和/或加速清除。