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    Capsule and tray systems for combined sample collection, archiving, purification, and PCR
    2.
    发明申请
    Capsule and tray systems for combined sample collection, archiving, purification, and PCR 审中-公开
    用于组合样品收集，归档，纯化和PCR的胶囊和托盘系统

    公开(公告)号：US20070190522A1

    公开(公告)日：2007-08-16

    申请号：US11725301

    申请日：2007-03-19

    申请人： Ralph Somack ,  Gary Lim

    发明人： Ralph Somack ,  Gary Lim

    IPC分类号： C12Q1/00 ,  C12Q1/68 ,  A01N1/02

    CPC分类号： B01D61/18 ,  B01D61/00 ,  B01L3/50255 ,  B01L2300/042 ,  B01L2300/0829

    摘要： The present invention relates to a biological sample collection, archiving, purification, and manipulating system and methods for collecting, archiving, purifying, and manipulating biological samples. The system can include a plurality of devices, each having a species-immobilizing filter within a tubular body. The body includes first and second end openings and one or more removable caps or sealing devices for sealing the end openings. A plurality of the devices can be positioned in an array tray for simultaneously archiving, purifying, or reacting respective samples collected in the respective devices. In another embodiment, a plate system is provided that includes a plate having a plurality of through-holes with each through-hole having a species-immobilizing filter disposed therein. The plate system also includes sealing devices such as sealing trays or removable caps for sealing first and second end openings of each through-hole of the plate.

    摘要翻译： 本发明涉及用于收集，归档，纯化和操作生物样品的生物样品收集，归档，纯化和操作系统和方法。 该系统可以包括多个装置，每个装置在管状体内具有物种固定的过滤器。 主体包括第一和第二端开口以及用于密封端部开口的一个或多个可拆卸盖或密封装置。 可以将多个装置定位在阵列托盘中，以便同时归档，净化或反应收集在各个装置中的各个样品。 在另一个实施例中，提供一种板系统，其包括具有多个通孔的板，每个通孔具有设置在其中的物种固定过滤器。 板系统还包括密封装置，例如用于密封板的每个通孔的第一和第二端开口的密封盘或可移除盖。

    Methods of nucleic acid isolation
    5.
    发明授权
    Methods of nucleic acid isolation 失效
    核酸分离方法

    公开(公告)号：US5972613A

    公开(公告)日：1999-10-26

    申请号：US987329

    申请日：1997-12-09

    申请人： Ralph Somack ,  Stephen E. Moring

    发明人： Ralph Somack ,  Stephen E. Moring

    IPC分类号： C12N15/09 ,  C07H1/06 ,  C07H21/00 ,  C12N15/10 ,  C12Q1/68

    CPC分类号： C12N15/1003 ,  C12Q1/6806 ,  Y10S435/803

    摘要： The invention relates to new methods of RNA isolation that exploit the surprising discovery that RNA may be differentially precipitated from DNA. The subject methods result in the formation of an RNA-containing precipitate that has an RNA content at least two fold enriched with respect to DNA, as compared with the RNA to DNA ratio of the solution from which the RNA-containing precipitate is derived. The invention also includes various methods of DNA isolation that employ the selective precipitation of RNA. The degree of RNA enrichment achieved is often much greater than two fold; enrichment by a factor of ten or greater is frequently obtained. By precipitating RNA from a solution, the RNA may be collected by simple procedures such as centrifugation or filtration, thereby avoiding the need to bind the RNA to solid phase. The collected RNA precipitate may then be solubilized. One embodiment of the subject methods involves forming an RNA-containing nucleic acid composition, adding an RNA precipitate forming chaotropic salt to the nucleic acid composition in a sufficient concentration to form an RNA-containing precipitate, collecting the RNA-containing precipitate, and (optionally) solubilizing the precipitate. Preferred precipitate forming chaotropic salts are guanidine hydrochloride and guanidine thiocyanate. Other embodiments of the invention include kits for performing the methods of the invention. The invention also includes systems for performing the methods of the invention.

    摘要翻译： 本发明涉及RNA分离的新方法，其利用RNA可能从DNA差异沉淀的惊人发现。 与衍生含RNA的沉淀物的溶液的RNA与DNA的比例相比，主题方法导致形成含RNA沉淀物，其RNA含量相对于DNA富集至少两倍。 本发明还包括采用RNA选择性沉淀的各种DNA分离方法。 实现的RNA浓缩程度通常远大于2倍; 浓缩十倍以上时经常得到。 通过从溶液中沉淀RNA，可以通过简单的方法如离心或过滤收集RNA，从而避免将RNA结合到固相。 然后可以将收集的RNA沉淀物溶解。 本发明方法的一个实施方案包括形成含RNA的核酸组合物，将足够浓度的形成离液盐的RNA沉淀物加入到核酸组合物中以形成含RNA沉淀物，收集含RNA沉淀物，和（任选地 ）溶解沉淀物。 形成离液盐的优选沉淀物是盐酸胍和硫氰酸胍。 本发明的其它实施方案包括用于实施本发明方法的试剂盒。 本发明还包括用于执行本发明的方法的系统。