公开(公告)号：US20240287484A1

公开(公告)日：2024-08-29

申请号：US18598627

申请日：2024-03-07

申请人： Metagenomi, Inc.

发明人： Brian C. THOMAS ,  Christopher BROWN ,  Daniela S.A. GOLTSMAN ,  Sarah LAPERRIERE ,  Cindy CASTELLE ,  Lisa ALEXANDER ,  Mary Kaitlyn Chiu ,  Morayma TEMOCHE-DIAZ ,  Anu THOMAS

IPC分类号： C12N9/12 ,  C12N9/22 ,  C12N15/10

CPC分类号： C12N9/1276 ,  C12N9/22 ,  C12N15/1096 ,  C12Y207/07049

摘要： The present disclosure provides systems and methods for transposing a cargo nucleotide sequence to a target nucleic acid site. These systems and methods can comprise a nucleic acid comprising the cargo nucleotide sequence, wherein the cargo nucleotide sequence is configured to interact with a retrotransposase, and the retrotransposase, wherein said retrotransposase is configured to transpose the cargo nucleotide sequence to the target nucleic acid site. The systems and methods can also involve use of functional fragments of retrotransposases.

公开(公告)号：US20240218339A1

公开(公告)日：2024-07-04

申请号：US18524511

申请日：2023-11-30

申请人： Metagenomi, Inc.

发明人： Brian THOMAS ,  Christopher BROWN ,  Audra DEVOTO ,  Cristina BUTTERFIELD ,  Lisa ALEXANDER ,  Daniela S.A. GOLTSMAN

IPC分类号： C12N9/22 ,  C12N15/11

CPC分类号： C12N9/22 ,  C12N15/111 ,  C12N2310/20

摘要： Described herein are methods, compositions, and systems derived from uncultivated microorganisms useful for gene editing.

公开(公告)号：US20230227857A1

公开(公告)日：2023-07-20

申请号：US17929978

申请日：2022-09-06

申请人： METAGENOMI, INC.

发明人： Brian THOMAS ,  Christopher BROWN ,  Audra DEVOTO ,  Cristina BUTTERFIELD ,  Lisa ALEXANDER ,  Daniela S.A. GOLTSMAN ,  Justine B. ALBERS ,  Alan BROOKS ,  Greg COST ,  Morayma TEMOCHE-DIAZ ,  Cindy CASTELLE ,  Rebecca LAMOTHE

IPC分类号： C12N15/90 ,  C12N15/11 ,  C12N9/22

CPC分类号： C12N15/907 ,  C12N15/11 ,  C12N15/111 ,  C12N9/22 ,  C12N15/902 ,  C12N2310/20 ,  C12N2800/80

摘要： Described herein are methods, compositions, and systems derived from uncultivated microorganisms useful for gene editing.

公开(公告)号：US20240344045A1

公开(公告)日：2024-10-17

申请号：US18668543

申请日：2024-05-20

申请人： METAGENOMI, INC.

发明人： Brian C. THOMAS ,  Christopher BROWN ,  Rose KANTOR ,  Audra DEVOTO ,  Cristina BUTTERFIELD ,  Lisa ALEXANDER ,  Daniela S.A. GOLTSMAN ,  Jason LIU

IPC分类号： C12N9/22 ,  C12N15/11 ,  C12N15/113

CPC分类号： C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C12N2310/20 ,  C12N2310/531 ,  C12N2800/80

摘要： The present disclosure provides for endonuclease enzymes having distinguishing domain features, as well as methods of using such enzymes or variants thereof.

公开(公告)号：US20240336905A1

公开(公告)日：2024-10-10

申请号：US18597260

申请日：2024-03-06

申请人： Metagenomi, Inc.

发明人： Brian C. THOMAS ,  Christopher BROWN ,  Cindy CASTELLE ,  Lisa ALEXANDER ,  Liliana GONZALEZ-OSORIO ,  Paula MATHEUS CARNEVALI ,  Dom CASTANZO

IPC分类号： C12N9/22 ,  C12N15/11 ,  C12N15/90

CPC分类号： C12N9/22 ,  C12N15/11 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/80

摘要： Described herein are methods, compositions, and systems derived from uncultivated microorganisms useful for gene editing.

公开(公告)号：US20240327871A1

公开(公告)日：2024-10-03

申请号：US18598610

申请日：2024-03-07

申请人： Metagenomi, Inc.

发明人： Brian C. THOMAS ,  Christopher BROWN ,  Daniela S.A. GOLTSMAN ,  Lisa ALEXANDER ,  Sarah LAPERRIERE

IPC分类号： C12N15/90 ,  C12N9/22

CPC分类号： C12N15/907 ,  C12N9/22 ,  C12N15/90 ,  C12N2800/90

摘要： The present disclosure provides systems and methods for transposing a cargo nucleotide sequence to a target nucleic acid site. These systems and methods may comprise a first double-stranded nucleic acid comprising the cargo nucleotide sequence, wherein the cargo nucleotide sequence is configured to interact with a transposase, and the transposase, wherein said transposase is configured to transpose the cargo nucleotide sequence to the target nucleic acid site.

公开(公告)号：US20240200047A1

公开(公告)日：2024-06-20

申请号：US18586929

申请日：2024-02-26

申请人： Metagenomi, Inc.

发明人： Brian C. THOMAS ,  Christopher BROWN ,  Rose KANTOR ,  Audra DEVOTO ,  Cristina BUTTERFIELD ,  Lisa ALEXANDER ,  Daniela S.A. GOLTSMAN ,  Jason LIU ,  Rebecca LAMOTHE ,  Diego ESPINOSA ,  Meghan STORLIE ,  Greg COST

IPC分类号： C12N9/22 ,  C12N15/10 ,  C12N15/11

CPC分类号： C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N2310/20

摘要： The present disclosure provides for endonuclease enzymes having distinguishing domain features, as well as methods of using such enzymes or variants thereof.

公开(公告)号：US20240360477A1

公开(公告)日：2024-10-31

申请号：US18598724

申请日：2024-03-07

申请人： Metagenomi, Inc.

发明人： Brian C. THOMAS ,  Christopher BROWN ,  Daniela S.A. GOLTSMAN ,  Sarah LAPERRIERE ,  Cindy CASTELLE ,  Lisa ALEXANDER ,  Mary Kaitlyn Chiu ,  Morayma TEMOCHE-DIAZ ,  Anu THOMAS

IPC分类号： C12N15/90 ,  C12N9/12

CPC分类号： C12N15/907 ,  C12N9/1276 ,  C12N15/90 ,  C12Y207/07049 ,  C12N2800/90

摘要： The present disclosure provides systems and methods for transposing a cargo nucleotide sequence to a target nucleic acid site. These systems and methods may comprise a first double-stranded nucleic acid comprising the cargo nucleotide sequence, wherein the cargo nucleotide sequence is configured to interact with a retrotransposase, and the retrotransposase, wherein said retrotransposase is configured to transpose the cargo nucleotide sequence to the target nucleic acid site.

公开(公告)号：US20240301374A1

公开(公告)日：2024-09-12

申请号：US18481769

申请日：2023-10-05

申请人： METAGENOMI, INC.

发明人： Brian THOMAS ,  Christopher BROWN ,  Daniela S.A. GOLTSMAN ,  Cristina BUTTERFIELD ,  Lisa ALEXANDER ,  Jason LIU

IPC分类号： C12N9/22 ,  C12N9/12 ,  C12N15/11

CPC分类号： C12N9/22 ,  C12N9/1241 ,  C12N15/11 ,  C12N2310/20

摘要： The present disclosure provides systems and methods for transposing a cargo nucleotide sequence to a target nucleic acid site. These systems and methods may comprise a first double-stranded nucleic acid comprising the cargo nucleotide sequence, wherein the cargo nucleotide sequence is configured to interact with a recombinase or transposase complex, a cas effector complex comprising a cas effector and at least one engineered guide polynucleotide configured to hybridize to the target nucleic acid site, and the recombinase or transposase complex wherein said recombinase or transposase complex is configured to recruit the cargo nucleotide to the target nucleic acid site.

公开(公告)号：US20240294948A1

公开(公告)日：2024-09-05

申请号：US18669712

申请日：2024-05-21

申请人： Metagenomi, Inc.

发明人： Brian C. THOMAS ,  Christopher BROWN ,  Audra DEVOTO ,  Cristina BUTTERFIELD ,  Lisa ALEXANDER ,  Daniela S.A. GOLTSMAN ,  Rebecca LAMOTHE

IPC分类号： C12N15/90 ,  C12N9/22 ,  C12N15/11

CPC分类号： C12N15/907 ,  C12N9/22 ,  C12N15/11 ,  C12N15/902 ,  C12N2310/20 ,  C12N2310/531 ,  C12N2800/80

摘要： The present disclosure provides for endonuclease enzymes as well as methods of using such enzymes or variants thereof.