公开(公告)号：US20080003615A1

公开(公告)日：2008-01-03

申请号：US11652809

申请日：2007-01-12

Applicant: Michael Sheetz ,  Samuel Wind

Inventor： Michael Sheetz ,  Samuel Wind

IPC: G01N33/53 ,  C12M1/00

CPC classification number: B82Y15/00 ,  B82Y30/00 ,  G01N33/54366 ,  G01N33/54373 ,  G01N33/6803

Abstract: Systems and methods for measuring nanometer level binding reactions are described herein. In some embodiments, the disclosed subject matter is directed to a device comprising (a) a substrate having a surface and (b) an ordered array of posts, pits, or patches over the surface, wherein the posts, pits, or patches are capable of binding a protein or small molecule ligand, and wherein the pitch between adjacent posts, pits, or patches is less than about 100 nm. In some other embodiments, the disclosed subject matter is also directed to methods for identifying the presence of an analyte in a fluid and to methods for measuring relative binding specificity or affinity between an analyte in a fluid and the posts, pits, or patches, using the device of the disclosed subject matter.

Abstract translation: 本文描述了用于测量纳米级结合反应的系统和方法。 在一些实施例中，所公开的主题涉及一种装置，其包括（a）具有表面的基底和（b）在表面上的柱，凹坑或贴片的有序阵列，其中柱，凹坑或贴片能够 结合蛋白质或小分子配体，并且其中相邻柱，凹坑或贴片之间的间距小于约100nm。 在一些其他实施方案中，所公开的主题还涉及用于鉴定流体中分析物的存在的方法以及用于测量流体中的分析物与柱，凹坑或贴片之间的相对结合特异性或亲和力的方法，使用 所公开的主题的装置。

公开(公告)号：US20070048790A1

公开(公告)日：2007-03-01

申请号：US11404716

申请日：2006-04-13

Applicant: Michael Sheetz ,  Samuel Wind

Inventor： Michael Sheetz ,  Samuel Wind

IPC: G01N33/53 ,  C12M3/00

CPC classification number: B82Y15/00 ,  B82Y30/00 ,  G01N33/54366 ,  G01N33/54373 ,  G01N33/6803

Abstract: The present invention is directed to a device comprising (a) a substrate having a surface and (b) an ordered array of posts over the surface, wherein the posts are capable of binding a protein or small molecule ligand, and wherein the pitch between adjacent posts is less than about 100 nm. The invention is also directed to methods for identifying the presence of an analyte in a fluid and to methods for measuring relative binding specificity or affinity between an analyte in a fluid and the posts, using the device of the present invention.

Abstract translation: 本发明涉及一种装置，其包括（a）具有表面的基底和（b）表面上的有序阵列的柱，其中所述柱能够结合蛋白质或小分子配体，并且其中相邻 柱小于约100nm。 本发明还涉及用于鉴定流体中分析物的存在的方法和使用本发明的装置测量流体中的分析物与柱之间的相对结合特异性或亲和力的方法。

公开(公告)号：US20070059645A1

公开(公告)日：2007-03-15

申请号：US11454638

申请日：2006-06-16

Applicant: Jinyao Tang ,  Samuel Wind

Inventor： Jinyao Tang ,  Samuel Wind

IPC: G03F7/26

CPC classification number: H01L51/102 ,  H01L21/31683 ,  H01L51/0083 ,  H01L51/0545 ,  Y10S977/89 ,  Y10S977/893 ,  Y10T29/49117

Abstract: The present invention relates to methods for fabricating nanoscale electrodes separated by a nanogap, wherein the gap size may be controlled with high precision using a self-aligning aluminum oxide mask, such that the gap width depends upon the thickness of the aluminum oxide mask. The invention also provides methods for using the nanoscale electrodes.

Abstract translation: 本发明涉及用纳米隙分离的纳米尺度电极的制造方法，其中使用自对准氧化铝掩模可以高精度地控制间隙尺寸，使得间隙宽度取决于氧化铝掩模的厚度。 本发明还提供了使用纳米级电极的方法。