公开(公告)号：US20050069947A1

公开(公告)日：2005-03-31

申请号：US10484031

申请日：2002-07-16

Applicant: Bernard Erlanger ,  Louis Brue ,  Michael Sheetz

Inventor： Bernard Erlanger ,  Louis Brue ,  Michael Sheetz

IPC: G01Q70/12 ,  A61K47/48 ,  C07K16/18 ,  C07K16/26 ,  C07K16/44 ,  C12P21/04 ,  C12P21/08 ,  G01Q70/18 ,  G01Q80/00 ,  G01N33/53 ,  C07K16/00

CPC classification number: C07K16/44 ,  A61K47/6835 ,  A61K47/6923 ,  A61K47/6949 ,  A61K51/0495 ,  A61K51/1268 ,  A61K2039/505 ,  B82Y5/00 ,  C07K16/18 ,  C07K16/26 ,  C07K2317/55 ,  C07K2317/56

Abstract: This invention provides two compositions. The first composition comprises a nanotube and at least one anti-nanotube antibody, wherein the anti-nanotube antibody is bound to the nanotube. The second composition comprises a fullerene and at least one anti-fullerene antibody, wherein the anti-fullerene antibody is bound to the fullerene. Finally, this invention provides methods and kits relating to the antibody and compositions of matter.

Abstract translation: 本发明提供两种组合物。 第一组合物包含纳米管和至少一种抗纳米管抗体，其中所述抗纳米管抗体与纳米管结合。 第二组合物包含富勒烯和至少一种抗富勒烯抗体，其中抗富勒烯抗体与富勒烯结合。 最后，本发明提供了与抗体和物质组成有关的方法和试剂盒。

公开(公告)号：US07575866B2

公开(公告)日：2009-08-18

申请号：US11219506

申请日：2005-09-01

Applicant: Virginia Cornish ,  Michael Sheetz ,  Larry Miller

Inventor： Virginia Cornish ,  Michael Sheetz ,  Larry Miller

IPC: C12Q1/68 ,  C12Q1/26 ,  G01N33/53 ,  G01N33/573

CPC classification number: G01N33/582 ,  C12Q1/025 ,  C12Q1/32 ,  G01N33/5005 ,  G01N33/573 ,  G01N2333/906 ,  G01N2500/10

Abstract: The present invention relates to methods and compositions which utilize binding relationships between detector molecules and their ligands for labeling molecules of interest in vivo. A “target” molecule is linked to a detector molecule (such as a protein or a nucleic acid) and the function and/or location of the target molecule is monitored by binding the detector molecule its ligand, which carries a detectable label. In particular embodiments of the invention, an intracellular fusion protein comprising a target protein and a detector protein is bound to a membrane-permeable, fluorescently-labeled ligand of the detector protein, thereby providing an adjunct or alternative to Green Fluorescent Protein.

Abstract translation: 本发明涉及使用检测器分子及其配体之间的结合关系的方法和组合物，用于在体内标记感兴趣的分子。 “靶”分子与检测器分子（例如蛋白质或核酸）连接，通过结合检测分子其配体，来监测靶分子的功能和/或位置，该配体携带可检测的标记。 在本发明的具体实施方案中，包含靶蛋白和检测蛋白的细胞内融合蛋白与检测蛋白的膜可渗透的荧光标记配体结合，从而提供绿色荧光蛋白的辅助物或替代物。

公开(公告)号：US20080003615A1

公开(公告)日：2008-01-03

申请号：US11652809

申请日：2007-01-12

Applicant: Michael Sheetz ,  Samuel Wind

Inventor： Michael Sheetz ,  Samuel Wind

IPC: G01N33/53 ,  C12M1/00

CPC classification number: B82Y15/00 ,  B82Y30/00 ,  G01N33/54366 ,  G01N33/54373 ,  G01N33/6803

Abstract: Systems and methods for measuring nanometer level binding reactions are described herein. In some embodiments, the disclosed subject matter is directed to a device comprising (a) a substrate having a surface and (b) an ordered array of posts, pits, or patches over the surface, wherein the posts, pits, or patches are capable of binding a protein or small molecule ligand, and wherein the pitch between adjacent posts, pits, or patches is less than about 100 nm. In some other embodiments, the disclosed subject matter is also directed to methods for identifying the presence of an analyte in a fluid and to methods for measuring relative binding specificity or affinity between an analyte in a fluid and the posts, pits, or patches, using the device of the disclosed subject matter.

Abstract translation: 本文描述了用于测量纳米级结合反应的系统和方法。 在一些实施例中，所公开的主题涉及一种装置，其包括（a）具有表面的基底和（b）在表面上的柱，凹坑或贴片的有序阵列，其中柱，凹坑或贴片能够 结合蛋白质或小分子配体，并且其中相邻柱，凹坑或贴片之间的间距小于约100nm。 在一些其他实施方案中，所公开的主题还涉及用于鉴定流体中分析物的存在的方法以及用于测量流体中的分析物与柱，凹坑或贴片之间的相对结合特异性或亲和力的方法，使用 所公开的主题的装置。

公开(公告)号：US20060211007A1

公开(公告)日：2006-09-21

申请号：US11219506

申请日：2005-09-01

Applicant: Virginia Cornish ,  Michael Sheetz ,  Larry Miller

Inventor： Virginia Cornish ,  Michael Sheetz ,  Larry Miller

IPC: C12Q1/68 ,  G01N33/53 ,  C12Q1/26

CPC classification number: G01N33/582 ,  C12Q1/025 ,  C12Q1/32 ,  G01N33/5005 ,  G01N33/573 ,  G01N2333/906 ,  G01N2500/10

Abstract: The present invention relates to methods and compositions which utilize binding relationships between detector molecules and their ligands for labeling molecules of interest in vivo. A “target” molecule is linked to a detector molecule (such as a protein or a nucleic acid) and the function and/or location of the target molecule is monitored by binding the detector molecule its ligand, which carries a detectable label. In particular embodiments of the invention, an intracellular fusion protein comprising a target protein and a detector protein is bound to a membrane-permeable, fluorescently-labeled ligand of the detector protein, thereby providing an adjunct or alternative to Green Fluorescent Protein.

公开(公告)号：US20070048790A1

公开(公告)日：2007-03-01

申请号：US11404716

申请日：2006-04-13

Applicant: Michael Sheetz ,  Samuel Wind

Inventor： Michael Sheetz ,  Samuel Wind

IPC: G01N33/53 ,  C12M3/00

CPC classification number: B82Y15/00 ,  B82Y30/00 ,  G01N33/54366 ,  G01N33/54373 ,  G01N33/6803

Abstract: The present invention is directed to a device comprising (a) a substrate having a surface and (b) an ordered array of posts over the surface, wherein the posts are capable of binding a protein or small molecule ligand, and wherein the pitch between adjacent posts is less than about 100 nm. The invention is also directed to methods for identifying the presence of an analyte in a fluid and to methods for measuring relative binding specificity or affinity between an analyte in a fluid and the posts, using the device of the present invention.

Abstract translation: 本发明涉及一种装置，其包括（a）具有表面的基底和（b）表面上的有序阵列的柱，其中所述柱能够结合蛋白质或小分子配体，并且其中相邻 柱小于约100nm。 本发明还涉及用于鉴定流体中分析物的存在的方法和使用本发明的装置测量流体中的分析物与柱之间的相对结合特异性或亲和力的方法。

公开(公告)号：US20050084843A1

公开(公告)日：2005-04-21

申请号：US10914924

申请日：2004-08-10

Applicant: Michael Sheetz ,  Yasuhiro Sawada

Inventor： Michael Sheetz ,  Yasuhiro Sawada

IPC: C12P21/04 ,  C12Q1/00 ,  G01N33/50 ,  G01N33/53 ,  G01N33/566 ,  G01N33/567 ,  G01N33/68

CPC classification number: G01N33/6842 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/502 ,  G01N33/5076 ,  G01N33/566 ,  G01N33/567 ,  G01N33/68

Abstract: The present invention relates to a cytoskeletal system composed of purified components which allow for the identification of key proteins involved in mechanotransduction. The system comprises a cytoskeleton network deficient in force-dependent proteins, exogenous proteins in contact with the cytoskeleton network, a means for applying a force to the cytoskeleton network, and a means for identifying any of the exogenous proteins that bind to the cytoskeleton network when a force is applied to the cytoskeleton network, wherein proteins identified are involved in mechnotransduction. The invention further relates to a method for identifying proteins involved in mechanotransduction using the cytoskeletal system of the present invention. The identification of key players involved in mechanotransduction allows for the identification of agents capable of inhibiting or enhancing mechanotransduction.

Abstract translation: 本发明涉及由纯化的组分组成的细胞骨架系统，其允许鉴定涉及机械转导的关键蛋白质。 该系统包含缺乏力依赖性蛋白质的细胞骨架网络，与细胞骨架网络接触的外源蛋白质，向细胞骨架网络施加力的方法，以及用于鉴定结合细胞骨架网络的任何外源蛋白质的方法， 将力施加到细胞骨架网络，其中所鉴定的蛋白质参与机体转导。 本发明还涉及使用本发明的细胞骨架系统鉴定涉及机械转导的蛋白质的方法。 识别涉及机械转导的关键参与者能够鉴定能够抑制或增强机械转导的药剂。