公开(公告)号：US5859340A

公开(公告)日：1999-01-12

申请号：US475427

申请日：1995-06-07

申请人： Michel Dubois ,  Rene Grison ,  Jean-Jacques Leguay ,  Annie Pignard ,  Alain Toppan

发明人： Michel Dubois ,  Rene Grison ,  Jean-Jacques Leguay ,  Annie Pignard ,  Alain Toppan

IPC分类号： A01H5/00 ,  C12N1/21 ,  C12N5/10 ,  C12N9/24 ,  C12N9/42 ,  C12N15/09 ,  C12N15/29 ,  C12N15/56 ,  C12N15/82 ,  C12N15/62

CPC分类号： C12Y302/01017 ,  C12N15/8279 ,  C12N9/2442 ,  C12Y302/01014

摘要： Recombinant gene coding for a protein having endochitinase activity or for a precursor thereof which comprises the sequence of SEQ ID NO:1 below:Gly Gly Asp Leu Gly Ser VaL Ile Ser Asn Ser Met Phe Asp Gln Met Leu Lys His Arg Asn Glu Asn Ser Cys Gln Gly Lys Asn Asn Phe Tyr Ser Tyr Asn Ala Phe Ile Thr Ala Ala Arg Ser Phe Pro Gly, Phe Gly Thr Ser Gly Asp Ile Asn Ala Arg Lys Arg Glu Ile Ala Ala Phe Phe Ala Gln Thr Ser His Glu Thr Thr Gly Gly Trp Pro Ser Ala Pro Asp Gly Pro Phe Ala Trp Gly Tyr Cys Phe Leu Arg Gly Arg Gly Asn Pro Gly Asp Tyr Cys Ser Pro Ser Ser Gln Trp Pro Cys Ala Pro Gly Arg Lys Tyr Phe Gly Arg Gly Pro Ile Gln Ile Ser His Asn Tyr Asn Tyr Gly Pro Cys Gly Arg Ala Ile Gly Val Asp Leu Leu Asn Asn Pro Asp Leu Val Ala Thr Asp Pro Val Ile Ser Phe Lys Thr Ala Ile Trp Phe Trp Met Thr Pro Gln Ser Pro Lys Pro Ser Cys His Asp Val Ile Ile Gly Arg Trp Asn Pro Ser Ala Gly Asp Arg Ser Ala Asn Arg Leu Pro Gly Phe Gly Val Ile Thr Asn Ile Ile Asn Gly Gly Leu Glu Cys Gly Arg Gly Asn Asp Asn Arg Val Gln Asp Arg Ile Gly Phe Tyr Arg Arg Tyr Cys Gly Ile Leu Gly Val Ser Pro Gly Asp Asn Leu Asp Cys Gly Asn Gln Arg Ser Phe Gly Asn Gly Leu Leu Val Asp Thr Met.Application: For obtaining plants resisting to pathogenic agents.

摘要翻译： 编码具有内切素酶活性的蛋白质或其前体的重组基因，其包含以下SEQ ID NO：1的序列：Gly Gly Asp Leu Gly Ser VaL Ile Ser Asn Ser Met Phe Asp Gln Met Leu Lys His Arg Asn Glu Asn Ser Cys Gln Gly Lys Asn Asn Phe Tyr Ser Tyr Asn Ala Phe Ile Thr Ala Ala Arg Ser Phe Pro Gly，Phe Gly Thr Ser Gly Asp Ile Asn Ala Arg Lys Arg Glu Ile Ala Ala Phe Phe Ala Gln Thr Ser His Glu Thr Thr Gly Gly Trp Pro Ser Ala Pro Asp Gly Pro Phe Ala Trp Gly Tyr Cys Phe Leu Arg Gly Arg Gly Asn Pro Gly Asp Tyr Cys Ser Pro Ser Ser Gln Trp Pro Cys Ala Pro Gly Arg Lys Tyr Phe Gly Arg Gly Pro Ile Gln Ile Ser 他的Asn Tyr Asn Tyr Gly Pro Cys Gly Arg Ala Ile Gly Val Asp Leu Leu Asn Asn Pro Asp Leu Val Ala Thr Asp Pro Val Ile Ser Phe Lys Thr Ala Ile Trp Phe Trp Met Thr Pro Gln Ser Pro Lys Pro Ser Cys His Asp Val Ile Ile Gly Arg Trp Asn Pro Ser Ala Gly Asp Arg Ser Ala Asn Arg Leu Pro Gly Phe Gly Val Ile Thr Asn Ile Ile Asn Gly Gly Leu Glu Cys Gly Arg Gly Asn Asp Asn A rg Val Gln Asp Arg Ile Gly Phe Tyr Arg Arg Tyr Cys Gly Ile Leu Gly Val Ser Pro Gly Asp Asn Leu Asp Cys Gly Asn Gln Arg Ser Phe Gly Asn Gly Leu Leu Val Asp Thr Met。 用途：用于获得抗病原体的植物。

公开(公告)号：US5932698A

公开(公告)日：1999-08-03

申请号：US842165

申请日：1992-05-01

申请人： Michel Dubois ,  Rene Grison ,  Jean-Jacques Leguay ,  Annie Pignard ,  Alain Toppan

发明人： Michel Dubois ,  Rene Grison ,  Jean-Jacques Leguay ,  Annie Pignard ,  Alain Toppan

IPC分类号： A01H5/00 ,  C12N1/21 ,  C12N5/10 ,  C12N9/24 ,  C12N9/42 ,  C12N15/09 ,  C12N15/29 ,  C12N15/56 ,  C12N15/82 ,  C12N15/62

CPC分类号： C12Y302/01017 ,  C12N15/8279 ,  C12N9/2442 ,  C12Y302/01014

摘要： Recombinant gene coding for a protein having endochitinase activity or for a precursor thereof which comprises the sequence (SEQ ID NO:1) below:Gly Gly Asp Leu Gly Ser Val Ile Ser Asn Ser Met PheAsp Gln Met Leu Lys His Arg Asn Glu Asn Ser Cys GlnGly Lys Asn Asn Phe Tyr Ser Tyr Asn Ala Phe Ile ThrAla Ala Arg Ser Phe Pro Gly Phe Gly Thr Ser Gly AspIle Asn Ala Arg Lys Arg Glu Ile Ala Ala Phe Phe AlaGln Thr Ser His Glu Thr Thr Gly Gly Trp Pro Ser AlaPro Asp Gly Pro Phe Ala Trp Gly Tyr Cys Phe Leu ArgGly Arg Gly Asn Pro Gly Asp Tyr Cys Ser Pro Ser SerGln Trp Pro Cys Ala Pro Gly Arg Lys Tyr Phe Gly ArgGly Pro Ile Gln Ile Ser His Asn Tyr Asn Tyr Gly ProCys Gly Arg Ala Ile Gly Val Asp Leu Leu Asn Asn ProAsp Leu Val Ala Thr Asp Pro Val Ile Ser Phe Lys ThrAla Ile Trp Phe Trp Met Thr Pro Gln Ser Pro Lys ProSer Cys His Asp Val Ile Ile Gly Arg Trp Asn Pro SerAla Gly Asp Arg Ser Ala Asn Arg Leu Pro Gly Phe GlyVal Ile Thr Asn Ile Ile Asn Gly Gly Leu Glu Cys GlyArg Gly Asn Asp Asn Arg Val Gln Asp Arg Ile Gly PheTyr Arg Arg Try Cys Gly Ile Leu Gly Val Ser Pro GlyAsp Asn Leu Asp Cys Gly Asn Gln Arg Ser Phe Gly AsnGly Leu Leu Val Asp Thr Met.Application: For obtaining plants resisting to pathogenic agents.

摘要翻译： PCT No.PCT / FR91 / 00607 Sec。 371日期：1992年5月1日 102（e）日期1992年5月1日PCT PCT 1991年7月24日PCT公布。 出版物WO92 / 01792 日期1992年2月6日编码含有内切素酶活性的蛋白质的重组基因或其前体包含以下序列（SEQ ID NO：1）： - Gly Gly Asp Leu Gly Ser Val Ile Ser Asn Ser Met Phe - Asp Asp Met Leu Lys His Arg Asn Glu Asn Ser Cys Gln - - Gly Lys Asn Asn Phe Tyr Ser Tyr Asn Ala Phe Ile Thr - - Ala Ala Arg Ser Phe Pro Gly Phe Gly Thr Ser Gly Asp - - Ile Asn Ala Arg Lys Arg Glu Ile Ala Ala Phe Phe Ala - - Gln Thr Ser His Glu Thr Thr Gly Gly Trp Pro Ser Ala - - Pro Asp Gly Pro Phe Ala Trp Gly Tyr Cys Phe Leu Arg - - Gly Arg Gly Asn Pro Gly Asp Tyr Cys Ser Pro Ser Ser - - Gln Trp Pro Cys Ala Pro Gly Arg Lys Tyr Phe Gly Arg - - Gly Pro Ile Gln Ile Ser His Asn Tyr Asn Tyr Gly Pro - - Cys Gly Arg Ala Ile Gly Val Asp Leu Leu Asn Asn Pro - - Asp Leu Val Ala Thr Asp Pro Val Ile Ser Phe Lys Thr - - Ala Ile Trp Phe Trp Met Thr Pro Gln Ser Pro Lys Pro - - Ser Cys His Asp Val Ile Ile Gly Arg Trp Asn Pro Ser - - Ala Gly Asp Arg Ser Ala Asn Arg Leu Pro Gly Phe Gly - - Val Ile Thr Asn Ile Ile Asn Gly Gly Leu Glu Cys Gly - - Arg Gly Asn Asp Asn Arg Val Gln Asp Arg Ile Gly Phe - - Tyr Arg Arg尝试Cys Gly Ile Leu Gly Val Ser Pro Gly - - Asp Asn Leu Asp Cys Gly Asn Gln Arg Ser Phe Gly Asn - - Gly Leu Leu Val Asp Thr Met。 - 应用：用于获得抗病原体的植物。

公开(公告)号：US5477001A

公开(公告)日：1995-12-19

申请号：US966187

申请日：1993-01-25

申请人： Catherine Sass ,  Jean-Jacques Leguay ,  Rene Grison ,  Alain Toppan

发明人： Catherine Sass ,  Jean-Jacques Leguay ,  Rene Grison ,  Alain Toppan

IPC分类号： A01H5/00 ,  C12N1/21 ,  C12N5/10 ,  C12N9/24 ,  C12N15/00 ,  C12N15/56 ,  C12N15/82 ,  C12R1/19 ,  C12N15/29

CPC分类号： C12Y302/01039 ,  C12N15/8279 ,  C12N9/2402 ,  C12Y302/01058

摘要： The invention provides a protein, isolated from soybean, having .beta.-1,3-glucanase activity and comprising the amino acid sequence of SEQ ID No: 1. DNA constructs encoding said protein, and transformed plants expressing the protein, are also provided.

摘要翻译： PCT No.PCT / FR92 / 00268 Sec。 371日期：1993年1月25日 102（e）日期1993年1月25日PCT提交1992年3月25日PCT公布。 出版物WO92 / 16632 本发明提供了一种从大豆中分离得到的具有β-1,3-葡聚糖酶活性并包含SEQ ID No：1的氨基酸序列的蛋白质。编码所述蛋白质的DNA构建体和表达该蛋白质的转化植物 蛋白质。