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    Microfluidic chip for high-throughput perfusion-based three-dimensional cell culture
    1.
    发明授权
    Microfluidic chip for high-throughput perfusion-based three-dimensional cell culture 有权
    微流控芯片用于高通量灌注型三维细胞培养

    公开(公告)号：US09068281B2

    公开(公告)日：2015-06-30

    申请号：US13241123

    申请日：2011-09-22

    申请人： Min-Hsien Wu ,  Shih-Siou Wang ,  Wan-Chen Tsai ,  Yu-Han Chang ,  Chia-Hsun Hsieh ,  Yen-Ting Liu ,  Zhanfeng Cui

    发明人： Min-Hsien Wu ,  Shih-Siou Wang ,  Wan-Chen Tsai ,  Yu-Han Chang ,  Chia-Hsun Hsieh ,  Yen-Ting Liu ,  Zhanfeng Cui

    IPC分类号： C40B60/12 ,  C12M3/06 ,  C12M1/34

    CPC分类号： C40B60/12 ,  C12M23/16 ,  C12M41/00 ,  C12M41/12 ,  C12M41/40

    摘要： A microfluidic chip for three-dimensional cell culture with high-throughput perfusion includes an array of cell culture units, each unit including a cell culture medium inlet hole connecting to one cell culture medium tank, at least one micro-bioreactor, at least one microchannel and at least one medium collection and analysis tank. Each medium collection and analysis tank is connected to an air chamber with an air channel and the air chamber has negative pressure source holes to generate negative pressure to drive the culture medium. The microfluidic chip also includes an intermediate plate connected to the bottom surface of the roof, and two bottom plates detachably assembled at the bottom of the intermediate plate. The first and second bottom surfaces have micro-bioreactors and cylindrical recessed slots and the intermediate plate has corresponding holes to achieve the goal of three-dimensional cell culture using minimum experimental resources with high-throughput perfusion.

    摘要翻译： 用于具有高通量灌注的三维细胞培养的微流体芯片包括一系列细胞培养单元，每个单元包括连接到一个细胞培养基罐的细胞培养基入口孔，至少一个微生物反应器，至少一个微通道 和至少一个介质收集和分析罐。 每个介质收集和分析罐连接到具有空气通道的空气室，并且空气室具有负压源孔以产生负压以驱动培养基。 微流体芯片还包括连接到屋顶底面的中间板，以及可拆卸地组装在中间板的底部的两个底板。 第一和第二底面具有微生物反应器和圆柱形凹槽，并且中间板具有相应的孔，以使用具有高通量灌注的最小实验资源实现三维细胞培养的目标。

    Microfluidic Chip for High-Throughput Perfusion-Based Three-Dimensional Cell Culture
    2.
    发明申请
    Microfluidic Chip for High-Throughput Perfusion-Based Three-Dimensional Cell Culture 有权
    用于高通量灌注的三维细胞培养的微流控芯片

    公开(公告)号：US20120231976A1

    公开(公告)日：2012-09-13

    申请号：US13241123

    申请日：2011-09-22

    申请人： Min-Hsien Wu ,  Shih-Siou Wang ,  Wan-Chen Tsai ,  Yu-Han Chang ,  Chia-Hsun Hsieh ,  Yen-Ting Liu ,  Zhanfeng Cui

    发明人： Min-Hsien Wu ,  Shih-Siou Wang ,  Wan-Chen Tsai ,  Yu-Han Chang ,  Chia-Hsun Hsieh ,  Yen-Ting Liu ,  Zhanfeng Cui

    IPC分类号： C40B60/14

    CPC分类号： C40B60/12 ,  C12M23/16 ,  C12M41/00 ,  C12M41/12 ,  C12M41/40

    摘要： A microfluidic chip for three-dimensional cell culture with high-throughput perfusion includes an array of cell culture units, each unit including a cell culture medium inlet hole connecting to one cell culture medium tank, at least one micro-bioreactor, at least one microchannel and at least one medium collection and analysis tank. Each medium collection and analysis tank is connected to an air chamber with an air channel and the air chamber has negative pressure source holes to generate negative pressure to drive the culture medium. The microfluidic chip also includes an intermediate plate connected to the bottom surface of the roof, and two bottom plates detachably assembled at the bottom of the intermediate plate. The first and second bottom surfaces have micro-bioreactors and cylindrical recessed slots and the intermediate plate has corresponding holes to achieve the goal of three-dimensional cell culture using minimum experimental resources with high-throughput perfusion.

    摘要翻译： 用于具有高通量灌注的三维细胞培养的微流体芯片包括一系列细胞培养单元，每个单元包括连接到一个细胞培养基罐的细胞培养基入口孔，至少一个微生物反应器，至少一个微通道 和至少一个介质收集和分析罐。 每个介质收集和分析罐连接到具有空气通道的空气室，并且空气室具有负压源孔以产生负压以驱动培养基。 微流体芯片还包括连接到屋顶底面的中间板，以及可拆卸地组装在中间板的底部的两个底板。 第一和第二底面具有微生物反应器和圆柱形凹槽，并且中间板具有相应的孔，以使用具有高通量灌注的最小实验资源实现三维细胞培养的目标。