公开(公告)号：US09068281B2

公开(公告)日：2015-06-30

申请号：US13241123

申请日：2011-09-22

申请人： Min-Hsien Wu ,  Shih-Siou Wang ,  Wan-Chen Tsai ,  Yu-Han Chang ,  Chia-Hsun Hsieh ,  Yen-Ting Liu ,  Zhanfeng Cui

发明人： Min-Hsien Wu ,  Shih-Siou Wang ,  Wan-Chen Tsai ,  Yu-Han Chang ,  Chia-Hsun Hsieh ,  Yen-Ting Liu ,  Zhanfeng Cui

IPC分类号： C40B60/12 ,  C12M3/06 ,  C12M1/34

CPC分类号： C40B60/12 ,  C12M23/16 ,  C12M41/00 ,  C12M41/12 ,  C12M41/40

摘要： A microfluidic chip for three-dimensional cell culture with high-throughput perfusion includes an array of cell culture units, each unit including a cell culture medium inlet hole connecting to one cell culture medium tank, at least one micro-bioreactor, at least one microchannel and at least one medium collection and analysis tank. Each medium collection and analysis tank is connected to an air chamber with an air channel and the air chamber has negative pressure source holes to generate negative pressure to drive the culture medium. The microfluidic chip also includes an intermediate plate connected to the bottom surface of the roof, and two bottom plates detachably assembled at the bottom of the intermediate plate. The first and second bottom surfaces have micro-bioreactors and cylindrical recessed slots and the intermediate plate has corresponding holes to achieve the goal of three-dimensional cell culture using minimum experimental resources with high-throughput perfusion.

摘要翻译： 用于具有高通量灌注的三维细胞培养的微流体芯片包括一系列细胞培养单元，每个单元包括连接到一个细胞培养基罐的细胞培养基入口孔，至少一个微生物反应器，至少一个微通道 和至少一个介质收集和分析罐。 每个介质收集和分析罐连接到具有空气通道的空气室，并且空气室具有负压源孔以产生负压以驱动培养基。 微流体芯片还包括连接到屋顶底面的中间板，以及可拆卸地组装在中间板的底部的两个底板。 第一和第二底面具有微生物反应器和圆柱形凹槽，并且中间板具有相应的孔，以使用具有高通量灌注的最小实验资源实现三维细胞培养的目标。

公开(公告)号：US08506812B2

公开(公告)日：2013-08-13

申请号：US13555668

申请日：2012-07-23

申请人： Yinhua Wan ,  Xiangrong Chen ,  Zhiguo Su ,  Guanghui Ma ,  Xiaoguang Jiao ,  Fei Shen ,  Zhanfeng Cui

发明人： Yinhua Wan ,  Xiangrong Chen ,  Zhiguo Su ,  Guanghui Ma ,  Xiaoguang Jiao ,  Fei Shen ,  Zhanfeng Cui

IPC分类号： B01D33/44 ,  B01D29/075

CPC分类号： B01D65/08 ,  B01D61/145 ,  B01D61/147 ,  B01D63/082 ,  B01D2321/26

摘要： The present invention relates to a membrane separation method and a relevant equipment, in particular to a method and an equipment for membrane separation utilizing concentration polarization during membrane filtration process, especially, to a concentration process and equipment and a drawer special for drawing a concentration polarization layer. The direct removal of the concentration polarization layer from membrane surface not only decreases the adverse influence of concentration polarization on membrane separation but also obtains concentrated retention components, thereby significantly improving the ability to maintain membrane flux, solving the twinborn problems concerning concentration polarization and membrane fouling during the membrane separation process, and achieving a high-efficiency concentration for retention components. The method and equipment of the present invention can be widely applied in various membrane techniques, in particular in a membrane separation process for concentrating biomacromolecule and organic micromolecule products such as sugars, organic acids and polypeptides etc.

摘要翻译： 本发明涉及一种膜分离方法和相关设备，特别涉及在膜过滤过程中利用浓度极化的膜分离方法和设备，特别涉及浓缩过程和设备以及特别用于绘制浓缩极化的抽屉 层。 从膜表面直接除去浓缩极化层不仅降低了浓度极化对膜分离的不利影响，而且获得了浓缩的保留成分，从而显着提高了保持膜通量的能力，解决了浓度极化和膜污染的双胎问题 在膜分离过程中，并且实现保留成分的高效浓缩。 本发明的方法和设备可以广泛地应用于各种膜技术中，特别是在用于浓缩生物大分子和有机小分子产物如糖，有机酸和多肽等的膜分离方法中。

公开(公告)号：US08252184B2

公开(公告)日：2012-08-28

申请号：US12443797

申请日：2008-03-05

申请人： Yinhua Wan ,  Xiangrong Chen ,  Zhiguo Su ,  Guanghui Ma ,  Xiaoguang Jiao ,  Fei Shen ,  Zhanfeng Cui

发明人： Yinhua Wan ,  Xiangrong Chen ,  Zhiguo Su ,  Guanghui Ma ,  Xiaoguang Jiao ,  Fei Shen ,  Zhanfeng Cui

IPC分类号： B01D29/075 ,  B01D33/44

CPC分类号： B01D65/08 ,  B01D61/145 ,  B01D61/147 ,  B01D63/082 ,  B01D2321/26

摘要： The present invention relates to a membrane separation method and a relevant equipment, in particular to a method and an equipment for membrane separation utilizing concentration polarization during membrane filtration process, especially, to a concentration process and equipment and a drawer special for drawing a concentration polarization layer. The direct removal of the concentration polarization layer from membrane surface not only decreases the adverse influence of concentration polarization on membrane separation but also obtains concentrated retention components, thereby significantly improving the ability to maintain membrane flux, solving the twinborn problems concerning concentration polarization and membrane fouling during the membrane separation process, and achieving a high-efficiency concentration for retention components. The method and equipment of the present invention can be widely applied in various membrane techniques, in particular in a membrane separation process for concentrating biomacromolecule and organic micromolecule products such as sugars, organic acids and polypeptides etc.

摘要翻译： 本发明涉及一种膜分离方法和相关设备，特别涉及在膜过滤过程中利用浓度极化的膜分离方法和设备，特别涉及浓缩过程和设备以及特别用于绘制浓缩极化的抽屉 层。 从膜表面直接除去浓缩极化层不仅降低了浓度极化对膜分离的不利影响，而且获得了浓缩的保留成分，从而显着提高了保持膜通量的能力，解决了浓度极化和膜污染的双胎问题 在膜分离过程中，并且实现保留成分的高效浓缩。 本发明的方法和设备可以广泛地应用于各种膜技术中，特别是在用于浓缩生物大分子和有机小分子产物如糖，有机酸和多肽等的膜分离方法中。

公开(公告)号：US20120040394A1

公开(公告)日：2012-02-16

申请号：US13121351

申请日：2009-09-28

申请人： Paul Raju ,  Zhanfeng Cui

发明人： Paul Raju ,  Zhanfeng Cui

IPC分类号： C12Q1/18 ,  C12Q1/02 ,  C07K16/18 ,  C12N5/09

CPC分类号： C12N5/0693 ,  C12N2533/74 ,  G01N33/5011 ,  G01N33/5088

摘要： A method of producing an in vitro microtumour comprising: seeding a colorectal neoplastic cell into a three dimensional scaffold comprising polysaccharide co-polymer; providing said cell with a culture medium that supports the growth thereof; and incubating said cell in said scaffold for a time sufficient for microtumors to form, wherein said polysaccharide copolymer comprises glutaronate and mannuronate.

摘要翻译： 一种产生体外微量肿瘤的方法，包括：将结肠直肠肿瘤细胞接种到包含多糖共聚物的三维支架中; 向所述细胞提供支持其生长的培养基; 并将所述细胞在所述支架中孵育足以形成微量的时间，其中所述多糖共聚物包含戊二酸和甘露糖醛酸盐。

公开(公告)号：US20120285889A1

公开(公告)日：2012-11-15

申请号：US13555668

申请日：2012-07-23

申请人： Yinhua Wan ,  Xiangrong Chen ,  Zhiguo Su ,  Guanghui Ma ,  Xiaoguang Jiao ,  Fei Shen ,  Zhanfeng Cui

发明人： Yinhua Wan ,  Xiangrong Chen ,  Zhiguo Su ,  Guanghui Ma ,  Xiaoguang Jiao ,  Fei Shen ,  Zhanfeng Cui

IPC分类号： B01D61/14 ,  B01D61/36

CPC分类号： B01D65/08 ,  B01D61/145 ,  B01D61/147 ,  B01D63/082 ,  B01D2321/26

摘要： The present invention relates to a membrane separation method and a relevant equipment, in particular to a method and an equipment for membrane separation utilizing concentration polarization during membrane filtration process, especially, to a concentration process and equipment and a drawer special for drawing a concentration polarization layer. The direct removal of the concentration polarization layer from membrane surface not only decreases the adverse influence of concentration polarization on membrane separation but also obtains concentrated retention components, thereby significantly improving the ability to maintain membrane flux, solving the twinborn problems concerning concentration polarization and membrane fouling during the membrane separation process, and achieving a high-efficiency concentration for retention components. The method and equipment of the present invention can be widely applied in various membrane techniques, in particular in a membrane separation process for concentrating biomacromolecule and organic micromolecule products such as sugars, organic acids and polypeptides etc.

摘要翻译： 本发明涉及一种膜分离方法和相关设备，特别涉及在膜过滤过程中利用浓度极化的膜分离方法和设备，特别涉及浓缩过程和设备以及特别用于绘制浓缩极化的抽屉 层。 从膜表面直接除去浓缩极化层不仅降低了浓度极化对膜分离的不利影响，而且获得了浓缩的保留成分，从而显着提高了保持膜通量的能力，解决了浓度极化和膜污染的双胎问题 在膜分离过程中，并且实现保留成分的高效浓缩。 本发明的方法和设备可以广泛地应用于各种膜技术中，特别是在用于浓缩生物大分子和有机小分子产物如糖，有机酸和多肽等的膜分离方法中。

公开(公告)号：US20120231976A1

公开(公告)日：2012-09-13

申请号：US13241123

申请日：2011-09-22

申请人： Min-Hsien Wu ,  Shih-Siou Wang ,  Wan-Chen Tsai ,  Yu-Han Chang ,  Chia-Hsun Hsieh ,  Yen-Ting Liu ,  Zhanfeng Cui

发明人： Min-Hsien Wu ,  Shih-Siou Wang ,  Wan-Chen Tsai ,  Yu-Han Chang ,  Chia-Hsun Hsieh ,  Yen-Ting Liu ,  Zhanfeng Cui

IPC分类号： C40B60/14

CPC分类号： C40B60/12 ,  C12M23/16 ,  C12M41/00 ,  C12M41/12 ,  C12M41/40

摘要： A microfluidic chip for three-dimensional cell culture with high-throughput perfusion includes an array of cell culture units, each unit including a cell culture medium inlet hole connecting to one cell culture medium tank, at least one micro-bioreactor, at least one microchannel and at least one medium collection and analysis tank. Each medium collection and analysis tank is connected to an air chamber with an air channel and the air chamber has negative pressure source holes to generate negative pressure to drive the culture medium. The microfluidic chip also includes an intermediate plate connected to the bottom surface of the roof, and two bottom plates detachably assembled at the bottom of the intermediate plate. The first and second bottom surfaces have micro-bioreactors and cylindrical recessed slots and the intermediate plate has corresponding holes to achieve the goal of three-dimensional cell culture using minimum experimental resources with high-throughput perfusion.

摘要翻译： 用于具有高通量灌注的三维细胞培养的微流体芯片包括一系列细胞培养单元，每个单元包括连接到一个细胞培养基罐的细胞培养基入口孔，至少一个微生物反应器，至少一个微通道 和至少一个介质收集和分析罐。 每个介质收集和分析罐连接到具有空气通道的空气室，并且空气室具有负压源孔以产生负压以驱动培养基。 微流体芯片还包括连接到屋顶底面的中间板，以及可拆卸地组装在中间板的底部的两个底板。 第一和第二底面具有微生物反应器和圆柱形凹槽，并且中间板具有相应的孔，以使用具有高通量灌注的最小实验资源实现三维细胞培养的目标。

公开(公告)号：US20100276347A1

公开(公告)日：2010-11-04

申请号：US12682556

申请日：2008-10-08

申请人： Zhanfeng Cui

发明人： Zhanfeng Cui

IPC分类号： C02F1/44 ,  B01D61/12

CPC分类号： C02F1/002 ,  C02F2303/04

摘要： A gravity driven portable water purification device (1) comprises a dirty water inlet (5) operative to comprise a head of dirty water, water filtration means (23) connected to the dirty water inlet (5), and a clean water tank (35) connected to an outlet of the water filtration means (23). The dirty water inlet (5), when the device (1) is in an operational condition, is arranged relative to the clean water tank (35) and the water filtration means (23) such that, in use of the device, dirty water is forced through the water filtration means (23) and into the clean water tank (35) by the gravitational force generated from the head of dirty water in the dirty water inlet (5). The water filtration means (23) comprises a cross-flow membrane filter in which the gravitational force from the head of dirty water also forces dirty water across the cross-flow membrane filter so as to force contaminants out of the filter.

摘要翻译： 重力驱动便携式水净化装置（1）包括可操作以包括脏水头的脏水入口（5），连接到脏水入口（5）的水过滤装置（23）和清洁水箱（35） ）连接到水过滤装置（23）的出口。 当装置（1）处于操作状态时，脏水入口（5）相对于清洁水箱（35）和水过滤装置（23）布置，使得在使用装置时，脏水 被迫通过水过滤装置（23）并通过污水入口（5）中脏水头部产生的重力进入清洁水箱（35）。 水过滤装置（23）包括横流膜过滤器，其中来自脏水头部的重力也迫使脏水穿过横流膜过滤器，以将污染物强迫从过滤器中排出。

公开(公告)号：US20100044310A1

公开(公告)日：2010-02-25

申请号：US12443797

申请日：2008-03-05

申请人： Yinhua Wan ,  Xiangrong Chen ,  Zhiguo Su ,  Guanghui Ma ,  Xiaoguang Jiao ,  Fei Shen ,  Zhanfeng Cui

发明人： Yinhua Wan ,  Xiangrong Chen ,  Zhiguo Su ,  Guanghui Ma ,  Xiaoguang Jiao ,  Fei Shen ,  Zhanfeng Cui

IPC分类号： B01D65/08 ,  B01D63/00 ,  B01D65/00

CPC分类号： B01D65/08 ,  B01D61/145 ,  B01D61/147 ,  B01D63/082 ,  B01D2321/26

摘要： The present invention relates to a membrane separation method and a relevant equipment, in particular to a method and an equipment for membrane separation utilizing concentration polarization during membrane filtration process, especially, to a concentration process and equipment and a drawer special for drawing a concentration polarization layer. The direct removal of the concentration polarization layer from membrane surface not only decreases the adverse influence of concentration polarization on membrane separation but also obtains concentrated retention components, thereby significantly improving the ability to maintain membrane flux, solving the twinborn problems concerning concentration polarization and membrane fouling during the membrane separation process, and achieving a high-efficiency concentration for retention components. The method and equipment of the present invention can be widely applied in various membrane techniques, in particular in a membrane separation process for concentrating biomacromolecule and organic micromolecule products such as sugars, organic acids and polypeptides etc.

摘要翻译： 本发明涉及一种膜分离方法和相关设备，特别涉及在膜过滤过程中利用浓度极化的膜分离方法和设备，特别涉及浓缩过程和设备以及特别用于绘制浓缩极化的抽屉 层。 从膜表面直接除去浓缩极化层不仅降低了浓度极化对膜分离的不利影响，而且获得了浓缩的保留成分，从而显着提高了保持膜通量的能力，解决了浓度极化和膜污染的双胎问题 在膜分离过程中，并且实现保留成分的高效浓缩。 本发明的方法和设备可以广泛地应用于各种膜技术中，特别是在用于浓缩生物大分子和有机小分子产物如糖，有机酸和多肽等的膜分离方法中。

公开(公告)号：US20070207537A1

公开(公告)日：2007-09-06

申请号：US11725546

申请日：2007-03-20

申请人： Zhanfeng Cui ,  Min-Hsein Wu ,  Xia Xu

发明人： Zhanfeng Cui ,  Min-Hsein Wu ,  Xia Xu

IPC分类号： C12M1/34 ,  C12Q1/20

CPC分类号： C12M23/12 ,  C12M23/24 ,  C12M23/34 ,  C12M29/10

摘要： A cell culture plate for perfused cell or tissue culture comprises one or more wells. The wells are formed from a biocompatible gas permeable polymer. Means are provided to perfuse each well.

摘要翻译： 用于灌注的细胞或组织培养物的细胞培养板包含一个或多个孔。 孔由生物相容的透气性聚合物形成。 提供用于灌注每个井的装置。