公开(公告)号：US09127309B2

公开(公告)日：2015-09-08

申请号：US13982299

申请日：2012-01-31

申请人： Ming-Chou Lee ,  Sergey V. Voronov ,  Laurence R. McCarthy ,  Lilly I. Kong ,  Vesselin Tenkov Diankov

发明人： Ming-Chou Lee ,  Sergey V. Voronov ,  Laurence R. McCarthy ,  Lilly I. Kong ,  Vesselin Tenkov Diankov

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6816 ,  C12Q1/6851 ,  C12Q2521/319 ,  C12Q2525/161 ,  C12Q2563/107 ,  C12Q2525/204 ,  C12Q2565/125

摘要： Described are approaches for the identification, detection, and quantification of nucleic acids in a biological sample. These methods are based, in part, on the elucidation of anomalous migration properties of short nucleic acid molecules when conjugated to a fluorescent label, such as fluorescein labels, such that a smaller nucleic acid reliably migrates slower than a larger nucleic acid under the same conditions of separation.

摘要翻译： 描述了用于生物样品中核酸的鉴定，检测和定量的方法。 这些方法部分地基于当与荧光标记（例如荧光素标记）缀合时阐明短核酸分子的异常迁移性质，使得较小的核酸在相同条件下可靠地迁移比较大的核酸慢 的分离。

公开(公告)号：US5155019A

公开(公告)日：1992-10-13

申请号：US225561

申请日：1988-07-28

申请人： Mark L. Sussman ,  Joseph E. Ahnell ,  Laurence R. McCarthy

发明人： Mark L. Sussman ,  Joseph E. Ahnell ,  Laurence R. McCarthy

IPC分类号： C12M1/34

CPC分类号： C12M41/34 ,  C12M41/36 ,  Y10S435/807 ,  Y10S435/808

摘要： A method and apparatus for detecting the presence of biological activity in a sample of material. In the method, a sample of the material is placed in a closed container with a growth medium which includes a carbon source which may be metabolized to produce carbon dioxide. The medium with the sample therein is exposed to conditions conducive to the occurrence of normal metabolic process for a period of time sufficient to cause production of CO.sub.2 by the metabolism of the carbon source. Thereafter, the presence of CO.sub.2 in the gaseous atmosphere above the medium is detected by measuring the infrared absorbance of the gaseous atmosphere with the container by passing an infrared beam through the container and the gaseous atmosphere and detecting the infrared absorbance of the gaseous atmosphere.

摘要翻译： 一种用于检测材料样品中生物活性的存在的方法和装置。 在该方法中，将材料的样品放置在具有生长培养基的密闭容器中，生长培养基包括可以被代谢以产生二氧化碳的碳源。 其中具有样品的培养基暴露于有利于正常代谢过程发生的条件下足以通过碳源的代谢产生CO 2的时间。 此后，通过使红外线通过容器和气体气氛并检测气体气氛的红外吸光度，通过用容器测量气体气氛的红外吸光度来检测介质上方的气体气氛中的CO 2的存在。

公开(公告)号：US4659658A

公开(公告)日：1987-04-21

申请号：US686190

申请日：1984-12-26

申请人： Laurence R. McCarthy ,  H. Mark Perks

发明人： Laurence R. McCarthy ,  H. Mark Perks

IPC分类号： G01N33/571 ,  G01N33/546 ,  G01N33/569

CPC分类号： G01N33/571 ,  Y10S435/81 ,  Y10S435/871 ,  Y10S435/975 ,  Y10S436/808 ,  Y10S436/827

摘要： A method for identification of a microorganism includes combining in a suitable fluid the microorganism and a latex containing a particle coated with about 1 to 150 .mu.g per ml of latex of a lectin specific for the microorganism. Binding of the microorganism and lectin causes agglutination which is detected. The microorganism is identified by the agglutination as that organism which is specific for the lectin. The invention includes a reagent which may be included with other materials in a kit of materials useful for performing the method of the invention.

摘要翻译： 用于鉴定微生物的方法包括在合适的流体中混合微生物和含有涂覆有约1至150μg/ ml的微生物特异性凝集素胶乳的颗粒的胶乳。 微生物和凝集素的结合引起检测到的凝集。 微生物被凝集鉴定为特异于凝集素的生物体。 本发明包括可以在用于执行本发明的方法的一组材料中的其它材料中包含的试剂。