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1.发明授权
公开(公告)号：US5155019A
公开(公告)日：1992-10-13
申请号：US225561
申请日：1988-07-28
IPC分类号： C12M1/34
CPC分类号： C12M41/34 , C12M41/36 , Y10S435/807 , Y10S435/808
摘要： A method and apparatus for detecting the presence of biological activity in a sample of material. In the method, a sample of the material is placed in a closed container with a growth medium which includes a carbon source which may be metabolized to produce carbon dioxide. The medium with the sample therein is exposed to conditions conducive to the occurrence of normal metabolic process for a period of time sufficient to cause production of CO.sub.2 by the metabolism of the carbon source. Thereafter, the presence of CO.sub.2 in the gaseous atmosphere above the medium is detected by measuring the infrared absorbance of the gaseous atmosphere with the container by passing an infrared beam through the container and the gaseous atmosphere and detecting the infrared absorbance of the gaseous atmosphere.
摘要翻译： 一种用于检测材料样品中生物活性的存在的方法和装置。 在该方法中，将材料的样品放置在具有生长培养基的密闭容器中，生长培养基包括可以被代谢以产生二氧化碳的碳源。 其中具有样品的培养基暴露于有利于正常代谢过程发生的条件下足以通过碳源的代谢产生CO 2的时间。 此后，通过使红外线通过容器和气体气氛并检测气体气氛的红外吸光度，通过用容器测量气体气氛的红外吸光度来检测介质上方的气体气氛中的CO 2的存在。
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公开(公告)号：US4971900A
公开(公告)日：1990-11-20
申请号：US171499
申请日：1988-03-21
申请人： Joseph E. Ahnell , H. Mark Perks , Mark L. Sussman , Gregory Tice
发明人： Joseph E. Ahnell , H. Mark Perks , Mark L. Sussman , Gregory Tice
CPC分类号： C12Q1/04 , C12M41/34 , C12M41/36 , C12Q2304/46 , Y10S435/807 , Y10T436/235
摘要： An apparatus and method for the detection of the growth of microorganisms through infrared analysis of a sample of the gas produced by growth of the microorganism is descirbed. In the method, a sample of the headspace gas in a vial containing a growth medium which has been inoculated with a sample suspected of containing a microorganism is removed and transferred to a sample cell where infrared analysis is used to determine the presence of carbon dioxide, if any, produced by the growth of the microorganism.
摘要翻译： 描述了通过微生物生长产生的气体样品的红外分析来检测微生物生长的装置和方法。 在该方法中，将含有接种了怀疑含有微生物的样品的生长培养基的小瓶中的顶空气体样品除去并转移到使用红外分析来测定二氧化碳的存在的样品池中， 如果有的话，由微生物的生长产生。
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3.发明授权Method for detecting the presence of biologically active agents utilizing .sup.13 C-labeled substrates 失效用13 C标记的底物检测生物活性剂的存在的方法
公开(公告)号：US4182656A
公开(公告)日：1980-01-08
申请号：US722270
申请日：1976-09-10
摘要： A sample of material to be tested for the presence of biologically active agents, such as bacteria, is introduced into a sealable container partially filled with a culture medium comprising a .sup.13 C-labeled fermentable substrate, the remainder of the container being filled with a culture gas, the container and its contents are subjected to conditions conducive to biological activity for a predetermined period sufficient for fermentation of the medium to produce carbon dioxide after which the ratio of .sup.13 CO.sub.2 to .sup.12 CO.sub.2 in the culture gas in the container is determined and compared to the initial ratio of .sup.13 CO.sub.2 to .sup.12 CO.sub.2 in the culture gas in order to detect any differences indicating the presence of biologically active agents in the sample.
摘要翻译： 将要测试的生物活性剂如细菌的待测试材料样品引入部分填充有包含13C标记的可发酵底物的培养基的可密封容器中，剩余的容器中填充培养气体 容器及其内容物经受有利于生物活性的条件一段足以发酵培养基以产生二氧化碳的预定时间，在此之后确定容器中培养气体中的13CO2至12CO2的比例，并将其与初始 比例为13CO2至12CO2，以检测指示样品中存在生物活性剂的任何差异。
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公开(公告)号：US4073691A
公开(公告)日：1978-02-14
申请号：US717351
申请日：1976-08-24
CPC分类号： C12Q1/04 , C12Q2304/46 , Y10S435/803 , Y10S435/807 , Y10S435/874
摘要： A sample of material to be tested for the presence of biologically active agents, such as bacteria, is introduced into a sealable container partially filled with a culture medium; the remainder of the container being filled with a culture gas, the container and its contents are subjected to conditions conducive to biological activity for a predetermined period sufficient for fermentation of the medium to produce at least one gaseous product after which the character of the culture gas in the container is determined and compared to the initial character of the culture gas in order to detect any differences caused by changes in the composition of the culture gas indicating the presence or absence of biologically active agents in the sample.
摘要翻译： 将要测试的生物活性剂如细菌的待测试材料样品引入部分填充有培养基的可密封容器中; 容器的剩余部分填充有培养气体，容器及其内容物经受有利于生物活性的条件达预定时间，足以使培养基发酵以产生至少一种气态产物，之后培养气体的特性 在容器中确定并与培养气体的初始特性进行比较，以便检测由样品中存在或不存在生物活性剂的培养气体组成变化引起的任何差异。
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公开(公告)号：US4321322A
公开(公告)日：1982-03-23
申请号：US160507
申请日：1980-06-18
申请人： Joseph E. Ahnell
发明人： Joseph E. Ahnell
IPC分类号： C12M1/34 , C12Q1/04 , G01N27/416 , G01N33/487
CPC分类号： C12Q1/04 , C12M41/28 , C12M41/36 , C12M41/46 , C12Q2304/44 , C12Q2304/80 , Y10S435/817
摘要： Reliable and rapid detection of microorganisms is accomplished in an electroanalytical cell using a pulsed voltammetric detection technique employing the growth medium as the electrolyte and analyte and using simple wire electrodes fabricated from readily available materials. Organism detection occurs as a consequence of the depletion of oxygen in the growth medium/electrolyte caused by aerobic metabolism. Times-to-detection vary with inoculum strength in a predictable fashion, permitting quantification of the organism in question when results are compared to those obtained using known inocula of the same organism. The low duty cycle of the pulsed measurement enables the determination of the relative redox potential in the same cell using the same set of electrodes in order to provide information which may be characteristic of the type of organism being studied.
摘要翻译： 使用采用生长培养基作为电解质和分析物的脉冲伏安检测技术，并使用由容易获得的材料制成的简单的线电极，在电分析细胞中实现微生物的可靠和快速检测。 有机体检测是由于需氧代谢引起的生长培养基/电解质中氧的消耗而发生的。 检测时间随着接种物强度的变化，以可预测的方式，当结果与使用同一生物体的已知接种物获得的结果进行比较时，允许对有问题的生物进行定量。 脉冲测量的低占空比使得能够使用相同的电极组来确定相同电池中的相对氧化还原电位，以便提供可能是正在研究的生物体类型的特征的信息。
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6.发明授权Apparatus for and method of detection of significant bacteriuria in urine samples through measurement of head space gas oxygen consumption in a closed-vial system 失效通过测量封闭样品瓶系统中头部空间气体氧气消耗来检测尿样中重要菌尿的装置和方法
公开(公告)号：US4220715A
公开(公告)日：1980-09-02
申请号：US930680
申请日：1978-08-03
申请人： Joseph E. Ahnell
发明人： Joseph E. Ahnell
CPC分类号： C12Q1/04 , C12M41/34 , C12M41/36 , C12Q2304/44
摘要： The detection of "significant bacteriuria" in a urine sample is accomplished by measuring the head space gas oxygen consumption in a closed vial system. A sample to be tested is introduced into a sealed vial containing a growth medium and the head space gas oxygen concentration initially measured. After a suitable incubation period, the oxygen concentration above the sample is again measured using a closed sample loop. A decrease in the head space oxygen concentration below a predetermined level indicates the presence of a significant quantity of oxygen consuming microorganisms in the sample.
摘要翻译： 通过测量封闭的小瓶系统中的头部空间气体氧气消耗量，可以检测尿样中的“显着菌尿”。 将待测试的样品引入含有生长培养基的密封小瓶中，并且首先测量顶空间气体氧浓度。 在合适的培养期后，使用封闭的样品环再次测量样品上方的氧浓度。 头部空间氧浓度低于预定水平的降低表明样品中存在大量耗氧微生物。
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公开(公告)号：US4152213A
公开(公告)日：1979-05-01
申请号：US776426
申请日：1977-03-10
申请人： Joseph E. Ahnell
发明人： Joseph E. Ahnell
摘要： A sample of material to be tested for the presence of oxygen consuming bacteria is introduced into a sealable container partially filled with a culture medium; the remainder of the container being filled with an oxygen containing gas, the container being connected to means for sensing the production of a vacuum therein. The container and its contents are subjected to conditions conducive to bacterial growth for a predetermined period sufficient for growth of bacteria to consume some of the oxygen, during which time the pressure of the gas in the container is monitored and compared to the initial pressure in order to detect the production of a vacuum due to consumption of oxygen by bacteria from the test sample, thereby indicating the presence or absence of oxygen consuming bacteria in the sample of test material.
摘要翻译： 将待测氧气消耗细菌的物质样品引入部分充满培养基的可密封容器中; 容器的剩余部分填充有含氧气体，该容器连接到用于感测其中产生真空的装置。 容器及其内容物经受有利于细菌生长的条件，其预定时间足以使细菌生长以消耗一些氧气，在此期间监测容器中的气体的压力并按比例与初始压力进行比较 检测来自测试样品的细菌由于氧而消耗的真空的产生，从而表明测试材料样品中是否存在耗氧细菌。
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