公开(公告)号：US5236827A

公开(公告)日：1993-08-17

申请号：US835009

申请日：1992-02-12

申请人： Mark L. Sussman ,  Stephen G. Wilson ,  Gregory Tice

发明人： Mark L. Sussman ,  Stephen G. Wilson ,  Gregory Tice

IPC分类号： C12M1/34 ,  C12Q1/04 ,  C12Q1/34

CPC分类号： C12Q1/04 ,  C12M41/46 ,  C12Q1/34 ,  C12Q2334/22 ,  C12Q2337/22 ,  Y10S435/968 ,  Y10S436/80

摘要： A carrier having at least one kinetics and fluorescence enhancing support and a dry substance selected from the group consisting of fluorogenic substrates, B methylumbelliferone, 7-amino-4-methyl coumarin, B-napthylamine, fluoroscein, and resorufin deposited on the support demonstrates substantial enhancement of hydrolysis kinetics and fluorescence over pure liquid systems. When the device has a plurality of supports and the supports have different fluorogenic substrates an enzyme rate-of-reaction profile representative of a microorganism in the suspension can be determined and used to identify the organism. The device can also be used to characterize enzymes expressed by other biological specimens.

摘要翻译： 具有至少一种动力学和荧光增强载体的载体和选自沉积在载体上的荧光底物，B亚甲基伞形酮，7-氨基-4-甲基香豆素，B-萘乙胺，氟代西林和resorufin的干物质显示出显着的 在纯液体系统上提高水解动力学和荧光。 当装置具有多个载体并且载体具有不同的荧光底物时，可以确定代表悬浮液中的微生物的酶速反应曲线并用于鉴定生物体。 该装置还可用于表征由其他生物标本表达的酶。

公开(公告)号：US4971900A

公开(公告)日：1990-11-20

申请号：US171499

申请日：1988-03-21

申请人： Joseph E. Ahnell ,  H. Mark Perks ,  Mark L. Sussman ,  Gregory Tice

发明人： Joseph E. Ahnell ,  H. Mark Perks ,  Mark L. Sussman ,  Gregory Tice

IPC分类号： C12M1/34 ,  C12Q1/04

CPC分类号： C12Q1/04 ,  C12M41/34 ,  C12M41/36 ,  C12Q2304/46 ,  Y10S435/807 ,  Y10T436/235

摘要： An apparatus and method for the detection of the growth of microorganisms through infrared analysis of a sample of the gas produced by growth of the microorganism is descirbed. In the method, a sample of the headspace gas in a vial containing a growth medium which has been inoculated with a sample suspected of containing a microorganism is removed and transferred to a sample cell where infrared analysis is used to determine the presence of carbon dioxide, if any, produced by the growth of the microorganism.

摘要翻译： 描述了通过微生物生长产生的气体样品的红外分析来检测微生物生长的装置和方法。 在该方法中，将含有接种了怀疑含有微生物的样品的生长培养基的小瓶中的顶空气体样品除去并转移到使用红外分析来测定二氧化碳的存在的样品池中， 如果有的话，由微生物的生长产生。

公开(公告)号：US5624849A

公开(公告)日：1997-04-29

申请号：US520998

申请日：1995-08-30

申请人： Bradley S. Thomas ,  Mark L. Sussman

发明人： Bradley S. Thomas ,  Mark L. Sussman

IPC分类号： G01N33/48 ,  A61B5/15 ,  A61J1/20 ,  G01N1/00 ,  G01N1/10 ,  B01L3/02

CPC分类号： B01L3/5635 ,  A61B5/15003 ,  A61B5/150305 ,  A61B5/150732 ,  A61B5/154 ,  A61J1/065 ,  B01L2300/0838 ,  B01L3/50825 ,  Y10T436/2575

摘要： The present invention relates to a method and apparatus for transferring fluid from a blood collection tube to a glass capillary tube. This eliminates the need for health care workers to open the blood collection tube, insert a glass capillary tube into the blood collection robe, pipet a fluid such as blood into the tube and be exposed to the excess fluid from the capillary tube. The apparatus of the present invention is directed to a transfer device, and a dispenser which can be used with the transfer device, for carrying out this method.

摘要翻译： 本发明涉及将流体从采血管传送到玻璃毛细管的方法和装置。 这消除了卫生保健工作者打开采血管的需要，将玻璃毛细管插入血液收集袍中，将诸如血液的流体移入管中并暴露于来自毛细管的多余的液体。 本发明的装置涉及一种能够与传送装置一起使用的传送装置和分配器，用于执行该方法。

公开(公告)号：US5155019A

公开(公告)日：1992-10-13

申请号：US225561

申请日：1988-07-28

申请人： Mark L. Sussman ,  Joseph E. Ahnell ,  Laurence R. McCarthy

发明人： Mark L. Sussman ,  Joseph E. Ahnell ,  Laurence R. McCarthy

IPC分类号： C12M1/34

CPC分类号： C12M41/34 ,  C12M41/36 ,  Y10S435/807 ,  Y10S435/808

摘要： A method and apparatus for detecting the presence of biological activity in a sample of material. In the method, a sample of the material is placed in a closed container with a growth medium which includes a carbon source which may be metabolized to produce carbon dioxide. The medium with the sample therein is exposed to conditions conducive to the occurrence of normal metabolic process for a period of time sufficient to cause production of CO.sub.2 by the metabolism of the carbon source. Thereafter, the presence of CO.sub.2 in the gaseous atmosphere above the medium is detected by measuring the infrared absorbance of the gaseous atmosphere with the container by passing an infrared beam through the container and the gaseous atmosphere and detecting the infrared absorbance of the gaseous atmosphere.

摘要翻译： 一种用于检测材料样品中生物活性的存在的方法和装置。 在该方法中，将材料的样品放置在具有生长培养基的密闭容器中，生长培养基包括可以被代谢以产生二氧化碳的碳源。 其中具有样品的培养基暴露于有利于正常代谢过程发生的条件下足以通过碳源的代谢产生CO 2的时间。 此后，通过使红外线通过容器和气体气氛并检测气体气氛的红外吸光度，通过用容器测量气体气氛的红外吸光度来检测介质上方的气体气氛中的CO 2的存在。