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公开(公告)号：US20050172205A1
公开(公告)日：2005-08-04
申请号：US10771823
申请日：2004-02-03
申请人： Ming-i Lin , Brian Connolly , Todd Leonard , Gregory Mann , Jonathan Raymond
发明人： Ming-i Lin , Brian Connolly , Todd Leonard , Gregory Mann , Jonathan Raymond
CPC分类号： H03M13/091
摘要： A CRC generator/checker for generating CRC results, comprising: a set of CRC circuits connected in series, each CRC circuit responsive to a different control signal generated by a control logic, each CRC circuit having a seed input adapted to receive a seed, a data input adapted to receive and process a different set of M-bits of a data unit and a result output adapted to generate a result, the result output of a previous CRC circuit connected to the seed input of an immediately subsequent CRC circuit, the seed input of a first CRC circuit connected to an output of a remainder register, an input of the remainder register connected to an output of a multiplexer, the result outputs of the multiplicity of CRC circuits connected to different inputs of the multiplexer, the multiplexer responsive to a select signal generated by the control logic.
摘要翻译： 一种用于生成CRC结果的CRC发生器/检验器，包括：串联连接的一组CRC电路，每个CRC电路响应于由控制逻辑产生的不同控制信号，每个CRC电路具有适于接收种子的种子输入， 数据输入，适于接收和处理数据单元的不同的M位组，以及适于产生结果的结果输出，连接到紧接着的CRC电路的种子输入的先前CRC电路的结果输出，种子 连接到余数寄存器的输出的第一CRC电路的输入，连接到多路复用器的输出的剩余寄存器的输入，结果连接到多路复用器的不同输入的多个CRC电路的输出，多路复用器响应于 由控制逻辑产生的选择信号。
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公开(公告)号：US5411876A
公开(公告)日：1995-05-02
申请号：US890300
申请日：1992-05-27
申请人： Will Bloch , Jonathan Raymond , Alan R. Read
发明人： Will Bloch , Jonathan Raymond , Alan R. Read
CPC分类号： C08J7/047 , B01L3/5082 , B29C59/103 , C08J2471/00 , C12Q1/6848 , G01N2035/00267 , G01N2035/00277
摘要： Improvements to the polymerase chain reaction (PCR), a process for in vitro enzymatic amplification of specific nucleic acid sequences, can be achieved by changing the way that PCR reagents are mixed and the enzymatic reaction is started and by the replacement of mineral oil, commonly used as a vapor barrier to minimize solvent evaporation, by a grease or wax. The use of such mixtures allows for the delay of reagent mixing until the first heating step of a PCR amplification, thereby reducing the enzymatic generation of nonspecific products which occurs when a complete mixture of PCR reagents, with or without test sample, stands at room temperature or below. These mixtures increase the shelf-life of PCR reagents and increase protection of the laboratory environment against contamination by PCR product.
摘要翻译： 聚合酶链反应（PCR）的改进，一种用于特异性核酸序列的体外酶促扩增的方法，可以通过改变PCR试剂混合和酶反应开始的方式以及通常更换矿物油来实现 用作蒸汽屏障以通过油脂或蜡最小化溶剂蒸发。 使用这种混合物允许试剂混合的延迟直到PCR扩增的第一加热步骤，从而减少当具有或不具有测试样品的PCR试剂的完全混合物在室温下存在时发生的非特异性产物的酶产生 或以下。 这些混合物增加了PCR试剂的保质期，并增加了实验室环境对PCR产物污染的保护。
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