公开(公告)号：US4767622A

公开(公告)日：1988-08-30

申请号：US524919

申请日：1983-08-19

申请人： Miodrag Ristic ,  Mary L. Chilbert

发明人： Miodrag Ristic ,  Mary L. Chilbert

IPC分类号： C12P21/00 ,  A61K39/00 ,  A61K39/015 ,  C07K14/445 ,  C12N1/10 ,  C07G7/00

CPC分类号： C12N1/10 ,  C07K14/445 ,  A61K39/00 ,  Y10S530/822

摘要： Disclosed are vaccine compositions for use in developing protective immunity against infection by Plasmodium parasites. Soluble proteinaceous immunogens are isolated from the fluid culture medium of in vitro propagated plasmodial species parasites (e.g., P.falciparum) in mammalian erythrocyte culture supernatant or from washes, including hypotonic washes, of cultured erythrocytes parasitized by plasmodium. Immunogens so obtained have molecular weights in the range from about 35,000 daltons to about 85,000 daltons. Two principal immunogens of the invention have molecular weights of about 42,000 and 54,000 daltons, respectively. The water soluble immunogens are administered in a suitable carrier such as isotonic salt solution and in combination with a suitable adjuvant such as saponin or, preferably, aluminum hydroxide. Upon administration to vertebrate animals susceptible to plasmodial infection, vaccines according to the invention provoke immune responses protective against morbidity and mortality caused by, e.g., P.falciparum infection. Disclosed also are procedures for optimization of large scale plasmodial parasite growth in human erythrocyte cultures with accompanying development of late erythrocytic stage parasites in large numbers and optimization of isolatable quantities of culture medium supernatant and wash-derived immunogens.

摘要翻译： 公开了用于开发针对疟原虫感染的保护性免疫的疫苗组合物。 可溶性蛋白质免疫原从哺乳动物红细胞培养上清液中的体外繁殖的疟原虫寄生虫（例如恶性疟原虫）的流体培养基中分离出来，或从疟原虫寄生的培养的红细胞洗涤（包括低渗洗涤）中分离。 如此获得的免疫原具有在约35,000道尔顿至约85,000道尔顿范围内的分子量。 本发明的两种主要免疫原分别具有约42,000和54,000道尔顿的分子量。 水溶性免疫原在合适的载体例如等渗盐溶液中并与合适的佐剂如皂苷或优选氢氧化铝组合施用。 在给予对疟原虫感染敏感的脊椎动物的动物时，根据本发明的疫苗引发了针对例如恶性疟原虫感染引起的发病率和死亡率的免疫应答。 还公开了用于优化人类红细胞培养物中大规模的疟原虫寄生虫生长的程序，伴随着大量晚期红细胞阶段寄生虫的发展，并优化了可分离量的培养基上清液和洗涤来源的免疫原。

公开(公告)号：US5855902A

公开(公告)日：1999-01-05

申请号：US192807

申请日：1994-02-07

申请人： Miodrag Ristic ,  Lionel Wanduragala

发明人： Miodrag Ristic ,  Lionel Wanduragala

IPC分类号： A61K39/002 ,  A23J3/20 ,  C07K14/44

CPC分类号： A61K39/002

摘要： A subunit vaccine for Tritrichomonas foetus and method for preparing such vaccine for use in immunizing and treating animals is provided. The method disclosed involves separating out the antigens by centrifuging homogenized Tritrichomonas foetus cells, preferably at about 830.times.g for about 15 minutes, solubilizing the antigen with an nonionic detergent and completing with saponin. Topical administration, such as intravaginal or intrapretutial administration, of such vaccine preparation in conjunction with subcutaneous administration in combination with other adjuvants is effective to eliminate infection.

摘要翻译： 提供了一种用于三氯胞嘧啶胎儿的亚单位疫苗和用于制备用于免疫和治疗动物的疫苗的方法。 所公开的方法包括通过离心匀浆的胎心细胞分离抗原，优选约830xg约15分钟，用非离子洗涤剂溶解抗原并用皂苷完成。 这种疫苗制剂与皮下给药结合其他佐剂的局部给药，例如阴道内或内分泌施用对消除感染是有效的。

公开(公告)号：US4596707A

公开(公告)日：1986-06-24

申请号：US130481

申请日：1980-03-31

申请人： Miodrag Ristic ,  Carlos Arellano

发明人： Miodrag Ristic ,  Carlos Arellano

IPC分类号： A61K39/018 ,  G01N33/569 ,  A61K39/00

CPC分类号： A61K39/018 ,  G01N33/56905

摘要： Soluble antigen, specific for Babesia parasites, is isolated from growth media of Babesia-infected erythrocytes in cell culture and characterized, inter alia, by its ability to inhibit the capacity of specific Babesia serum antibodies to neutralize infectivity of Babesia merozoites in vitro. Antigen preparations provide immunologically active components useful for vaccination of animals against infection by Babesia parasites and may be employed in the preparation of diagnostic reagents.

摘要翻译： 特异于巴贝虫寄生虫的可溶性抗原从细胞培养物中的巴贝虫感染的红细胞的生长培养基中分离出来，其特征在于其能够抑制特异性巴贝虫血清抗体在体外中和巴贝虫裂殖子的感染性的能力。 抗原制剂提供免疫活性成分，用于疫苗接种疫苗以抵抗巴贝虫寄生虫的感染，并可用于制备诊断试剂。

公开(公告)号：US4307191A

公开(公告)日：1981-12-22

申请号：US130482

申请日：1980-03-31

申请人： Miodrag Ristic ,  Michael G. Levy

发明人： Miodrag Ristic ,  Michael G. Levy

IPC分类号： A61K39/018 ,  C12N1/10 ,  C12Q1/18

CPC分类号： A61K39/018 ,  C12N1/10 ,  C12Q1/18 ,  Y10S435/947

摘要： A method for quantitative propagation of hemotropic parasites, especially Babesia parasites, in vitro, Babesia-infected erythrocyte cultures are incubated in an environment of enhanced carbon dioxide tension wherein the culture medium overlay provides a barrier to oxygen transmission such that cellular hemoglobin is essentially maintained in its deoxy state. By varying environmental carbon dioxide tension, merozoite stages of the parasite are selectively reversibly induced to migration between residence in host cells or culture medium, rendering possible the isolation of infective antigenic merozoites in large quantities. Specific Babesia antigen is harvested in large quantity from the in vitro culture medium.

摘要翻译： 血液寄生虫，特别是巴贝虫寄生虫，体外，巴贝虫感染的红细胞培养物的定量增殖的方法在增强的二氧化碳张力的环境中温育，其中培养基覆盖物提供对氧传递的屏障，使得细胞血红蛋白基本上保持在 其脱氧状态。 通过改变环境二氧化碳的张力，寄生虫的裂殖子阶段被选择性地可逆地诱导在宿主细胞或培养基中的迁移之间，从而可能大量分离感染性抗原性裂殖子。 从体外培养基中大量收获特异性巴贝虫抗原。