公开(公告)号：US09120105B2

公开(公告)日：2015-09-01

申请号：US13664967

申请日：2012-10-31

申请人： Monika Weber ,  Mark A. Reed

发明人： Monika Weber ,  Siu Lung Lo ,  Hazael Fabrizio Montanaro Ochoa ,  Christopher Daniel Yerino ,  Mark A. Reed

IPC分类号： G01N27/447 ,  B03C5/02 ,  G01N33/487 ,  B03C5/00 ,  B01L3/00 ,  G01N27/414

CPC分类号： B03C5/024 ,  B01L3/502753 ,  B01L2300/0874 ,  B01L2400/0406 ,  B01L2400/0424 ,  B01L2400/0487 ,  B03C5/005 ,  B03C2201/26 ,  G01N27/4145 ,  G01N33/487

摘要： An apparatus and method for separating an analyte from a test sample, such as bacteria from blood components, based on their dielectric properties, localizing or condensing the analyte, flushing substantially all remaining waste products from the test sample, and detecting low concentrations of the analyte. Species movement is caused by a module array imparting opposing dielectrophoretic forces. The module array includes a plurality of microfluidic channels with connecting microfluidic waste channels for directing undesired material away from the analyte. An electric field is applied causing a positive dielectrophoretic force to the analyte to capture the analyte. The Clausius-Mossotti factor of the analyte is changed by flushing the analyte with a reference solution, which causes a negative dielectrophoretic force to facilitate release of the analyte. A field effect nanowire or nanoribbon sensor detects the analyte after capture.

摘要翻译： 基于它们的介电特性，将分析物从测试样品例如细菌分离出来的装置和方法，定位或冷凝分析物，基本上冲洗来自测试样品的所有剩余废物，以及检测低浓度的分析物 。 物种移动是由赋予相反介电电泳力的模块阵列引起的。 模块阵列包括具有连接微流体废物通道的多个微流体通道，用于将不期望的材料引导离开分析物。 施加电场使得分析物具有正的介电电泳力以捕获分析物。 通过用参比溶液冲洗分析物来改变分析物的克劳修斯 - 莫索蒂（Clausius-Mossotti）因子，这引起负介电电泳力以促进分析物的释放。 场效应纳米线或纳米纤维传感器在捕获后检测分析物。

公开(公告)号：US20130105317A1

公开(公告)日：2013-05-02

申请号：US13664967

申请日：2012-10-31

申请人： Monika Weber ,  Siu Lung Lo ,  Hazael Fabrizio Montanaro Ochoa ,  Christopher Daniel Yerino ,  Mark A. Reed

发明人： Monika Weber ,  Siu Lung Lo ,  Hazael Fabrizio Montanaro Ochoa ,  Christopher Daniel Yerino ,  Mark A. Reed

IPC分类号： G01N27/447 ,  B82Y15/00

CPC分类号： B03C5/024 ,  B01L3/502753 ,  B01L2300/0874 ,  B01L2400/0406 ,  B01L2400/0424 ,  B01L2400/0487 ,  B03C5/005 ,  B03C2201/26 ,  G01N27/4145 ,  G01N33/487

摘要： An apparatus and method for separating an analyte from a test sample, such as bacteria from blood components, based on their dielectric properties, localizing or condensing the analyte, flushing substantially all remaining waste products from the test sample, and detecting low concentrations of the analyte. Species movement is caused by a module array imparting opposing dielectrophoretic forces. The module array includes a plurality of microfluidic channels with connecting microfluidic waste channels for directing undesired material away from the analyte. An electric field is applied causing a positive dielectrophoretic force to the analyte to capture the analyte. The Clausius-Mossotti factor of the analyte is changed by flushing the analyte with a reference solution, which causes a negative dielectrophoretic force to facilitate release of the analyte. A field effect nanowire or nanoribbon sensor detects the analyte after capture.

摘要翻译： 基于它们的介电特性，将分析物从测试样品例如细菌分离出来的装置和方法，定位或冷凝分析物，基本上冲洗来自测试样品的所有剩余废物，以及检测低浓度的分析物 。 物种移动是由赋予相反介电电泳力的模块阵列引起的。 模块阵列包括具有连接微流体废物通道的多个微流体通道，用于将不期望的材料引导离开分析物。 施加电场使得分析物具有正的介电电泳力以捕获分析物。 通过用参比溶液冲洗分析物来改变分析物的克劳修斯 - 莫索蒂（Clausius-Mossotti）因子，这引起负介电电泳力以促进分析物的释放。 场效应纳米线或纳米纤维传感器在捕获后检测分析物。

公开(公告)号：US20150107999A1

公开(公告)日：2015-04-23

申请号：US14582525

申请日：2014-12-24

申请人： Monika Weber ,  Mark A. Reed

发明人： Monika Weber ,  Siu Lung Lo ,  Hazael Fabrizio Montanaro Ochoa ,  Christopher Daniel Yerino ,  Mark A. Reed

IPC分类号： G01N27/447 ,  G01N27/414

CPC分类号： B01L3/502753 ,  B01L2300/0874 ,  B01L2400/0406 ,  B01L2400/0424 ,  B01L2400/0487 ,  B03C5/005 ,  B03C5/024 ,  B03C2201/18 ,  B03C2201/26 ,  G01N27/4145

摘要： An apparatus for separating an analyte from a test sample, such as bacteria from blood components, based on their dielectric properties, localizing or condensing the analyte, flushing substantially all remaining waste products from the test sample, and detecting low concentrations of the analyte. The module array includes a plurality of microfluidic channels with connecting microfluidic waste channels for directing undesired material away from the analyte. An electric field is applied causing a positive dielectrophoretic force to the analyte to capture the analyte. The electric field is applied to at least one electrode having a plurality of concentric rings or concentric arcs extending radially outwards from a center point, electrically connected to a voltage source such that when voltage is applied to the at least one electrode, the concentric rings or concentric arcs alternate in voltage potential.

摘要翻译： 基于它们的介电特性，将分析物从测试样品（例如细菌）中分离出来，将分析物定位或冷凝，基本上将所有剩余废物从测试样品中冲洗，并检测低浓度的分析物。 模块阵列包括具有连接微流体废物通道的多个微流体通道，用于将不期望的材料引导离开分析物。 施加电场使得分析物具有正的介电电泳力以捕获分析物。 电场被施加到至少一个电极，该至少一个电极具有多个同心圆或同心圆弧，其中心点从中心点径向向外延伸，电中连接到电压源，使得当电压施加到至少一个电极时，同心环或 同心弧交替电压。