公开(公告)号：US20130095488A1

公开(公告)日：2013-04-18

申请号：US13686320

申请日：2012-11-27

Applicant: Monsanto Technology LLC

Inventor： Natalia N. Bogdanova ,  David R. Corbin ,  Thomas M. Malvar ,  Frederick J. Perlak ,  James K. Roberts ,  Charles P. Romano

IPC: C07K14/325

CPC classification number: C07K14/325 ,  C12N15/8286 ,  Y02A40/162

Abstract: The present invention provides nucleotide sequences encoding an insecticidal protein exhibiting lepidopteran inhibitory activity, as well as a novel insecticidal protein referred to herein as a Cry1A.105 insecticide, transgenic plants expressing the insecticide, and methods for detecting the presence of the nucleotide sequences or the insecticide in a biological sample.

公开(公告)号：US20140194306A1

公开(公告)日：2014-07-10

申请号：US14102397

申请日：2013-12-10

Applicant: Monsanto Technology LLC

Inventor： Scott E. Andersen ,  Glenn R. Hicks ,  Joseph E. Huesing ,  Charles P. Romano ,  Clayton S. Vetsch

IPC: C07K14/435 ,  C12Q1/68

CPC classification number: C07K14/43563 ,  C12Q1/6888

Abstract: Expressed Sequence Tags (ESTs) isolated from the Western Corn Rootworm, Diabrotica virgifera virgifera LeConte, are disclosed. The invention encompasses nucleic acid molecules that encode D. v. virgifera protein homologs and fragments thereof. In addition, antibodies capable of binding the proteins are encompassed by the present invention. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, fragments of proteins, and antibodies, for example, for gene identification and analysis, and preparation of constructs.

Abstract translation: 公开了从西部玉米根虫，Diabrotica virgifera virgifera LeConte分离的表达序列标签（EST）。 本发明包括编码D.V. virgifera蛋白质同系物及其片段的核酸分子。 此外，本发明涵盖能够结合蛋白质的抗体。 所公开的EST在分离基因和启动子方面具有特别的用途，鉴定和绘制参与发育和代谢途径的基因以及确定基因功能。 EST为靶基因和分离新基因提供了独特的分子工具，用于植物保护和改良。 本发明还涉及使用所公开的核酸分子，蛋白质，蛋白质片段和抗体的方法，例如用于基因鉴定和分析以及构建体的制备。