公开(公告)号：US20240209385A1

公开(公告)日：2024-06-27

申请号：US18596328

申请日：2024-03-05

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  Andrei Y. Kouranov ,  Rosemarie Kuehn ,  Richard J. Lawrence ,  Ervin D. Nagy ,  Linda Rymarquis ,  Veena Veena

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N15/8216

Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for us for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.

公开(公告)号：US20220135991A1

公开(公告)日：2022-05-05

申请号：US17503235

申请日：2021-10-15

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  Andrei Y. Kouranov ,  Rosemarie Kuehn ,  Richard J. Lawrence ,  Ervin D. Nagy ,  Linda Rymarquis ,  Veena Veena

IPC: C12N15/82

Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.

公开(公告)号：US20230212596A1

公开(公告)日：2023-07-06

申请号：US18146434

申请日：2022-12-26

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  Andrei Y. Kouranov ,  Rosemarie Kuehn ,  Richard J. Lawrence ,  Ervin D. Nagy ,  Linda Rymarquis ,  Veena Veena

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N15/8216

Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.

公开(公告)号：US11578334B2

公开(公告)日：2023-02-14

申请号：US16758335

申请日：2018-10-24

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Ervin D. Nagy

IPC: C12N15/82

Abstract: This disclosure provides an engineered system comprising: a first nucleic acid molecule encoding a CasX nuclease, and a guide RNA (gRNA) or a second nucleic acid molecule encoding the gRNA, where the first nucleic acid molecule is codon optimized for a eukaryotic cell, and where the gRNA is designed to hybridize with a target site in the eukaryotic cell. Further, this disclosure provides a method of modifying at least one target site in a eukaryotic genome comprising: providing a eukaryotic cell with a CasX nuclease or a first nucleic acid molecule encoding the CasX nuclease, and providing the eukaryotic cell with a guide RNA (gRNA) or a second nucleic acid molecule encoding the gRNA, where the gRNA and the CasX nuclease form a complex, where the gRNA hybridizes to the target site, and where the complex generates a modification at the target site.

公开(公告)号：US20220372502A1

公开(公告)日：2022-11-24

申请号：US17731856

申请日：2022-04-28

Applicant: Monsanto Technology LLC

Inventor： Ian W. Davis ,  Matthew S. Marengo ,  Ervin D. Nagy

IPC: C12N15/82

Abstract: The invention provides novel synthetic small nuclear RNA (snRNA) promoters which are useful for CRISPR-mediated targeted gene modifications in plants. The invention also provides methods and compositions for use for the snRNA promoters driving expression of guide RNAs and non-coding RNAs for development of plants and plant cells comprising modified genomes.

公开(公告)号：US11952578B2

公开(公告)日：2024-04-09

申请号：US18146434

申请日：2022-12-26

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  Andrei Y. Kouranov ,  Rosemarie Kuehn ,  Richard J. Lawrence ,  Ervin D. Nagy ,  Linda Rymarquis ,  Veena Veena

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N15/8216

Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.

公开(公告)号：US11566254B2

公开(公告)日：2023-01-31

申请号：US17503235

申请日：2021-10-15

Applicant: Monsanto Technology LLC

Inventor： Brent Brower-Toland ,  Andrei Y. Kouranov ,  Rosemarie Kuehn ,  Richard J. Lawrence ,  Ervin D. Nagy ,  Linda Rymarquis ,  Veena Veena

IPC: C12N15/82

Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.

公开(公告)号：US11186843B2

公开(公告)日：2021-11-30

申请号：US15120110

申请日：2015-02-27

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Brent Brower-Toland ,  Andrei Y. Kouranov ,  Rosemarie Kuehn ,  Richard J. Lawrence ,  Ervin D. Nagy ,  Linda Rymarquis ,  Veena Veena

IPC: C12N15/81 ,  C12N15/82

Abstract: The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.