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    Pesticidal Nucleic Acids and Proteins and Uses Thereof
    7.
    发明申请
    Pesticidal Nucleic Acids and Proteins and Uses Thereof 审中-公开
    杀虫核酸和蛋白质及其用途

    公开(公告)号：US20160200778A1

    公开(公告)日：2016-07-14

    申请号：US15085005

    申请日：2016-03-30

    Applicant: Monsanto Technology LLC

    Inventor： David J. Bowen ,  Gregory J. Bunkers ,  Catherine Chay ,  John W. Pitkin ,  Timothy J. Rydel ,  Eric J. Sturman ,  Uma R. Sukuru ,  Brook Van Scoyoc ,  Stanislaw Flasinski

    IPC: C07K14/325 ,  C12N15/82 ,  A01N63/02

    CPC classification number: C12N15/8285 ,  A01N63/02 ,  C07K14/325 ,  C12N15/8286 ,  Y02A40/164

    Abstract: The invention provides compositions comprising polynucleotide molecules encoding certain pesticidal polypeptides which exhibit plant parasitic nematode and/or insect control properties, and are particularly directed to controlling plant parasitic pest species of nematodes and insects known to infest crop plant species. Methods for controlling pests are disclosed in which the toxic proteins are provided in the diet of the targeted plant pests. The invention also provides compositions such as nucleic acids, proteins, and plant and bacterial cells, plants, and seeds containing the nucleic acid and protein compositions, as well as methods and kits for identifying, detecting, and isolating the compositions of the present invention. The invention further provides a method of producing crops from recombinant seeds which contain the polynucleotide molecules encoding the pesticidal polypeptides of the present invention.

    Abstract translation: 本发明提供了包含编码某些农药多肽的多核苷酸分子的组合物，其表现出植物寄生线虫和/或昆虫控制性质，并且特别涉及控制已知侵染作物植物物种的线虫和昆虫的植物寄生虫种。 公开了用于控制害虫的方法，其中毒性蛋白质在目标植物害虫的饮食中提供。 本发明还提供了组合物，例如核酸，蛋白质，以及含有核酸和蛋白质组合物的植物和细菌细胞，植物和种子，以及用于鉴定，检测和分离本发明组合物的方法和试剂盒。 本发明还提供了一种从重组种子生产作物的方法，其含有编码本发明的杀虫多肽的多核苷酸分子。

    Secreted Insecticidal Protein and Gene Compositions From Bacillus Thuringiensis and Uses Therefor
    8.
    发明申请
    Secreted Insecticidal Protein and Gene Compositions From Bacillus Thuringiensis and Uses Therefor 审中-公开
    来自苏云金芽孢杆菌的分泌的杀虫蛋白和基因组成及其用途

    公开(公告)号：US20130212748A1

    公开(公告)日：2013-08-15

    申请号：US13682774

    申请日：2012-11-21

    Applicant: MONSANTO TECHNOLOGY LLC

    Inventor： Judith C. Donovan ,  William P. Donovan ,  James T. Engleman ,  Thomas M. Malvar ,  John W. Pitkin

    IPC: C07K14/32 ,  C12N15/82

    CPC classification number: C07K14/32 ,  C07K14/325 ,  C12N15/8286

    Abstract: The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against lepidopteran insects including European corn borer (ECB), tobacco budworm (TBW) and diamondback moth (DBM). Insecticidal proteins encoded by nucleotide sequences that hybridize under stringent conditions to the isolated and characterized nucleotide sequences are disclosed. Methods are disclosed for making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.

    Abstract translation: 本发明涉及分离和表征编码从苏云金芽孢杆菌和相关菌株分泌到细胞外空间中的新型杀虫蛋白的核苷酸序列。 从苏云金芽孢杆菌和相关菌株的培养上清液中分离蛋白质，并显示对包括欧洲玉米螟（ECB），烟草芽孢杆菌（TBW）和小菜蛾（DBM）等鳞翅目昆虫的杀虫活性。 公开了在严格条件下与分离和表征的核苷酸序列杂交的核苷酸序列编码的杀虫蛋白。 公开了制备和使用包含本发明的新型核苷酸序列的转基因细胞和植物的方法。

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